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- W2034144095 abstract "Proteins in nuclear extracts of HeLa cells that constitutively bound in vitro to three regions upstream of the interferon-inducible gene 6-16 were separated partially by chromatography on DEAE-Sepharose. Region one, a CCAAT box in the non-coding strand at position --63 to --67, was protected from DNase digestion by the bound protein(s) and was required for transcription in vitro. Region two, a tandem duplication sequence at position --89 to --168 contains two copies of a sequence essential for strong induction of the 6-16 gene by interferon in vitro. Region three, a palindromic sequence at position --449 to --465, not necessary for induction of 6-16 by interferon, was also protected from DNase digestion by nuclear protein(s). Templates with or without regions of two and three were transcribed equally well in extracts from interferon-treated or untreated cells." @default.
- W2034144095 created "2016-06-24" @default.
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- W2034144095 date "1989-12-04" @default.
- W2034144095 modified "2023-10-13" @default.
- W2034144095 title "Constitutive in vitro binding of nuclear proteins to the 5'-fanking region of 6-16, a human gene inducible by α, β-interferons" @default.
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- W2034144095 doi "https://doi.org/10.1016/0014-5793(89)81685-0" @default.
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