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- W2034150877 abstract "Abstract Adenosine deaminase (EC 3.5.4.4) (ADA) is assayed by incubating [8-14C]adenosine with the enzyme containing solution for 2 hr at pH 7.4 and 37°C. The trichloroacetic acid protein free filtrate is acid hydrolyzed and separated on a 2.5 × 0.2 cm2 Dowex-50 column where the hypoxanthine is eluted quantitatively in the first 15 ml of N HCl and counted. This assay is about 1000 times more sensitive than the spectrophotometric. It is linear up to 200 μIU/sample, reproducible, lends itself to serial determinations, and can be performed on turbid solutions." @default.
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- W2034150877 date "1976-03-01" @default.
- W2034150877 modified "2023-09-26" @default.
- W2034150877 title "A sensitive radiochemical assay for adenosine deaminase" @default.
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- W2034150877 doi "https://doi.org/10.1016/0090-1229(76)90022-2" @default.
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