FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034152019> ?p ?o ?g. }

• W2034152019 endingPage "5965" @default.
• W2034152019 startingPage "5965" @default.
• W2034152019 abstract "Purpose.: In proliferative vitreoretinopathy retinal pigment epithelial (RPE) cells undergo epithelial-mesenchymal transformation (EMT). Vitreous and transforming growth factor-β (TGFβ) have been implicated in this EMT. The role of TGFβ in the vitreous-mediated transformation of low-passage human RPE cells was investigated. Methods.: Cells were treated with vitreous or TGFβ2. SB431542 was used to inhibit TGFβ signaling. Morphology was investigated using phase-contrast or confocal microscopy. Motility was measured using a monolayer-wounding assay. Invasion was determined using basement membrane matrix-based assays. Gene expression was measured by quantitative PCR, immunohistochemistry, or immunoblotting. Results.: Changes in phosphorylation or cellular localization of Smad -2, -3, or -4 indicated a TGFβ-like activity in vitreous. Cortical actin filaments in untreated cells were replaced by stress fibers after TGFβ treatment, but peripheral actin aggregates were seen in vitreous-treated cells. SB431542 did not block the morphologic change induced by vitreous. Vitreous-treated cells exhibited increased motility and invasion, whereas TGFβ-treated cells did not. However, SB431542 decreased vitreous-meditated changes in motility and invasion. The levels of mRNA for genes indicative of myofibroblast differentiation (α-SMA and CTGF) were increased by treatment with TGFβ but suppressed by vitreous. TGFβ or vitreous caused increased expression of Snail1. Conclusions.: Vitreous or TGFβ caused a fibroblast-like morphology and induced Snail1, a marker of EMT. TGFβ activity in vitreous was necessary but not sufficient for the vitreous-induced motile, invasive phenotype. However, differences in the cytoskeletal organization and in the expression of CTGF and α-SMA suggested that TGFβ-treatment caused differentiation along a myofibroblast pathway, whereas vitreous treatment suppressed myofibroblast formation." @default.
• W2034152019 created "2016-06-24" @default.
• W2034152019 creator A5000309930 @default.
• W2034152019 creator A5008218308 @default.
• W2034152019 creator A5008269120 @default.
• W2034152019 creator A5008551171 @default.
• W2034152019 creator A5021554125 @default.
• W2034152019 creator A5040643758 @default.
• W2034152019 creator A5050833839 @default.
• W2034152019 creator A5075889094 @default.
• W2034152019 date "2009-12-01" @default.
• W2034152019 modified "2023-09-27" @default.
• W2034152019 title "Differential Effects of TGFβ and Vitreous on the Transformation of Retinal Pigment Epithelial Cells" @default.
• W2034152019 cites W107272129 @default.
• W2034152019 cites W1492896001 @default.
• W2034152019 cites W1551637241 @default.
• W2034152019 cites W1578782402 @default.
• W2034152019 cites W1597613207 @default.
• W2034152019 cites W1714595 @default.
• W2034152019 cites W1964941505 @default.
• W2034152019 cites W1966740850 @default.
• W2034152019 cites W1969740494 @default.
• W2034152019 cites W19734096 @default.
• W2034152019 cites W1976547960 @default.
• W2034152019 cites W1978146894 @default.
• W2034152019 cites W1978391747 @default.
• W2034152019 cites W1980325398 @default.
• W2034152019 cites W1981089517 @default.
• W2034152019 cites W1981970388 @default.
• W2034152019 cites W1984223367 @default.
• W2034152019 cites W1985395754 @default.
• W2034152019 cites W1987291878 @default.
• W2034152019 cites W1996211554 @default.
• W2034152019 cites W2000099153 @default.
• W2034152019 cites W2001651412 @default.
• W2034152019 cites W2008659893 @default.
• W2034152019 cites W2018137555 @default.
• W2034152019 cites W2022592853 @default.
• W2034152019 cites W2029243363 @default.
• W2034152019 cites W2033269379 @default.
• W2034152019 cites W2035851417 @default.
• W2034152019 cites W2039298921 @default.
• W2034152019 cites W2041747549 @default.
• W2034152019 cites W2047495851 @default.
• W2034152019 cites W2047992261 @default.
• W2034152019 cites W2049528696 @default.
• W2034152019 cites W2051812986 @default.
• W2034152019 cites W2056229778 @default.
• W2034152019 cites W2058667985 @default.
• W2034152019 cites W2059509871 @default.
• W2034152019 cites W2061211760 @default.
• W2034152019 cites W2061793781 @default.
• W2034152019 cites W2067192388 @default.
• W2034152019 cites W2071787763 @default.
• W2034152019 cites W2072610754 @default.
• W2034152019 cites W2076583198 @default.
• W2034152019 cites W2087367170 @default.
• W2034152019 cites W2095393157 @default.
• W2034152019 cites W2096188936 @default.
• W2034152019 cites W2099249565 @default.
• W2034152019 cites W2101914197 @default.
• W2034152019 cites W2102103202 @default.
• W2034152019 cites W2105683352 @default.
• W2034152019 cites W2108244474 @default.
• W2034152019 cites W2114232337 @default.
• W2034152019 cites W2122808924 @default.
• W2034152019 cites W2125355416 @default.
• W2034152019 cites W2134203671 @default.
• W2034152019 cites W2136280424 @default.
• W2034152019 cites W2151121496 @default.
• W2034152019 cites W2151948287 @default.
• W2034152019 cites W2153797982 @default.
• W2034152019 cites W2154568166 @default.
• W2034152019 cites W2155866571 @default.
• W2034152019 cites W2170115375 @default.
• W2034152019 cites W2278158612 @default.
• W2034152019 cites W2284004837 @default.
• W2034152019 cites W2411663067 @default.
• W2034152019 cites W2460489864 @default.
• W2034152019 cites W156445773 @default.
• W2034152019 doi "https://doi.org/10.1167/iovs.09-3621" @default.
• W2034152019 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19578024" @default.
• W2034152019 hasPublicationYear "2009" @default.
• W2034152019 type Work @default.
• W2034152019 sameAs 2034152019 @default.
• W2034152019 citedByCount "62" @default.
• W2034152019 countsByYear W20341520192012 @default.
• W2034152019 countsByYear W20341520192013 @default.
• W2034152019 countsByYear W20341520192014 @default.
• W2034152019 countsByYear W20341520192015 @default.
• W2034152019 countsByYear W20341520192016 @default.
• W2034152019 countsByYear W20341520192017 @default.
• W2034152019 countsByYear W20341520192018 @default.
• W2034152019 countsByYear W20341520192019 @default.
• W2034152019 countsByYear W20341520192020 @default.
• W2034152019 countsByYear W20341520192021 @default.
• W2034152019 countsByYear W20341520192022 @default.
• W2034152019 crossrefType "journal-article" @default.

• next