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• W2034152833 abstract "Abstract An average of about one molecule of a 26,500-dalton protein (termed Ht protein) has been found per tobacco mosaic virus (TMV) virion. Ht protein has been detected in five strains of TMV (U1, K, Cc, GA, and trace amounts in U2) prepared from tobacco by a variety of isolation methods and purification treatments, followed by incubation of virions with either 5% Triton X-100, 80 mM EDTA, 0.2 M phosphate or any of several proteolytic enzymes. Ht protein is associated with the insoluble RNA after disruption of virions with cold 66% acetic acid; it will also associate or co-precipitate in vitro with other added RNAs under the same conditions, apparently in a nonspecific manner. This protein is considered to be coded for by the host rather than the virus, based on two observations: (1) Ht protein from strain Cc TMV propagated in tobacco is smaller than the protein extracted from the same strain isolated from legumes; (2) peptide analysis after limited proteolysis under denaturing conditions showed that the Ht proteins isolated from two very different TMV strains (U1 and Cc) propagated in a common host were identical. If viral RNAs had coded for the Ht proteins, their amino acid sequences would have been expected to be different because of the known very dissimilar nucleotide sequences of the RNAs of the two strains. By comparing different classes of virus rods in which the coat protein subunits had been partially stripped from one end of the molecule (5′ end), it was concluded that Ht protein is not associated with a specific site in the virion. It is not preferentially associated with or absent from that proportion of TMV virions which are resistant to alkali stripping. Its location within the virions is not known, but it may be either intercalated between the coat proteins subunits or, more probably, lodged in the hole in the center of the virion. Its role in viral replication and pathogenicity, if any, remains to be determined." @default.
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• W2034152833 date "1978-11-01" @default.
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• W2034152833 title "Characterization of a second protein associated with virions of tobacco mosaic virus" @default.
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• W2034152833 doi "https://doi.org/10.1016/0042-6822(78)90365-3" @default.
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