Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034154092> ?p ?o ?g. }
- W2034154092 endingPage "127" @default.
- W2034154092 startingPage "114" @default.
- W2034154092 abstract "The tetra-anionic form of ATP (ATP4−) is known to induce monovalent and divalent ion fluxes in cells that express purinergic P2X7 receptors and with sustained application of ATP it has been shown that dyes as large as 831 Da can permeate the cell membrane. The current study explores the kinetics of loading α,α-trehalose (342 Da) into ATP stimulated J774.A1 cells, which are known to express the purinergic P2X7 receptor. Cells that were incubated at 37 °C in a 50 mM phosphate buffer (pH 7.0) containing 225 mM trehalose and 5 mM ATP, were shown to load trehalose linearly over time. Concentrations of ∼50 mM were reached within 90 min of incubation. Cells incubated in the same solution at 4 °C loaded minimally, consistent with the inactivity of the receptor at low temperatures. However, extended incubation at 37 °C (>60 min) resulted in zero next-day survival, with adverse effects appearing even with incubation periods as short as 30 min. By using a two-step protocol with a short time period at 37 °C to allow pore formation, followed by an extended loading period on ice, cells could be loaded with up to 50 mM trehalose while maintaining good next day recovery (49 ± 12% by Trypan blue exclusion, 56 ± 20% by alamarBlue assay). Cells porated by this method and allowed an overnight recovery period exhibited improved dehydration tolerance suggesting a role for ATP poration in the anhydrous preservation of cells." @default.
- W2034154092 created "2016-06-24" @default.
- W2034154092 creator A5045296273 @default.
- W2034154092 creator A5048362205 @default.
- W2034154092 creator A5061101062 @default.
- W2034154092 creator A5071152874 @default.
- W2034154092 creator A5071178482 @default.
- W2034154092 creator A5075867487 @default.
- W2034154092 creator A5076591617 @default.
- W2034154092 creator A5080982333 @default.
- W2034154092 date "2006-02-01" @default.
- W2034154092 modified "2023-10-03" @default.
- W2034154092 title "Trehalose uptake through P2X7 purinergic channels provides dehydration protection" @default.
- W2034154092 cites W1545462020 @default.
- W2034154092 cites W1560836239 @default.
- W2034154092 cites W1579992296 @default.
- W2034154092 cites W1815757713 @default.
- W2034154092 cites W1967718728 @default.
- W2034154092 cites W1970613983 @default.
- W2034154092 cites W1974987281 @default.
- W2034154092 cites W1981914844 @default.
- W2034154092 cites W1989345028 @default.
- W2034154092 cites W1994123472 @default.
- W2034154092 cites W2000223285 @default.
- W2034154092 cites W2002354885 @default.
- W2034154092 cites W2023676435 @default.
- W2034154092 cites W2049820913 @default.
- W2034154092 cites W2050069684 @default.
- W2034154092 cites W2050787847 @default.
- W2034154092 cites W2060823909 @default.
- W2034154092 cites W2063951483 @default.
- W2034154092 cites W2070908828 @default.
- W2034154092 cites W2072531995 @default.
- W2034154092 cites W2079734908 @default.
- W2034154092 cites W2085678065 @default.
- W2034154092 cites W2088236509 @default.
- W2034154092 cites W2089234603 @default.
- W2034154092 cites W2090628815 @default.
- W2034154092 cites W2093597737 @default.
- W2034154092 cites W2103520139 @default.
- W2034154092 cites W2106417317 @default.
- W2034154092 cites W2107195846 @default.
- W2034154092 cites W2108286208 @default.
- W2034154092 cites W2108605550 @default.
- W2034154092 cites W2113151858 @default.
- W2034154092 cites W2135708014 @default.
- W2034154092 cites W2158577731 @default.
- W2034154092 cites W2161848017 @default.
- W2034154092 cites W2169027510 @default.
- W2034154092 cites W2184812363 @default.
- W2034154092 cites W4211044690 @default.
- W2034154092 cites W4231685787 @default.
- W2034154092 cites W4238236627 @default.
- W2034154092 cites W4243388207 @default.
- W2034154092 cites W4249423410 @default.
- W2034154092 cites W4375906095 @default.
- W2034154092 doi "https://doi.org/10.1016/j.cryobiol.2005.10.009" @default.
- W2034154092 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16338230" @default.
- W2034154092 hasPublicationYear "2006" @default.
- W2034154092 type Work @default.
- W2034154092 sameAs 2034154092 @default.
- W2034154092 citedByCount "68" @default.
- W2034154092 countsByYear W20341540922012 @default.
- W2034154092 countsByYear W20341540922013 @default.
- W2034154092 countsByYear W20341540922014 @default.
- W2034154092 countsByYear W20341540922015 @default.
- W2034154092 countsByYear W20341540922016 @default.
- W2034154092 countsByYear W20341540922017 @default.
- W2034154092 countsByYear W20341540922018 @default.
- W2034154092 countsByYear W20341540922019 @default.
- W2034154092 countsByYear W20341540922020 @default.
- W2034154092 countsByYear W20341540922021 @default.
- W2034154092 countsByYear W20341540922022 @default.
- W2034154092 crossrefType "journal-article" @default.
- W2034154092 hasAuthorship W2034154092A5045296273 @default.
- W2034154092 hasAuthorship W2034154092A5048362205 @default.
- W2034154092 hasAuthorship W2034154092A5061101062 @default.
- W2034154092 hasAuthorship W2034154092A5071152874 @default.
- W2034154092 hasAuthorship W2034154092A5071178482 @default.
- W2034154092 hasAuthorship W2034154092A5075867487 @default.
- W2034154092 hasAuthorship W2034154092A5076591617 @default.
- W2034154092 hasAuthorship W2034154092A5080982333 @default.
- W2034154092 hasBestOaLocation W20341540922 @default.
- W2034154092 hasConcept C12554922 @default.
- W2034154092 hasConcept C143701427 @default.
- W2034154092 hasConcept C1491633281 @default.
- W2034154092 hasConcept C170493617 @default.
- W2034154092 hasConcept C178790620 @default.
- W2034154092 hasConcept C185592680 @default.
- W2034154092 hasConcept C25642318 @default.
- W2034154092 hasConcept C2778452849 @default.
- W2034154092 hasConcept C2778769901 @default.
- W2034154092 hasConcept C2780070996 @default.
- W2034154092 hasConcept C43617362 @default.
- W2034154092 hasConcept C55493867 @default.
- W2034154092 hasConcept C57306754 @default.
- W2034154092 hasConcept C74884574 @default.
- W2034154092 hasConcept C86803240 @default.