FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034157156> ?p ?o ?g. }

• W2034157156 endingPage "1163" @default.
• W2034157156 startingPage "1154" @default.
• W2034157156 abstract "G‐protein‐coupled receptor kinase 2 (GRK2) is known to specifically phosphorylate the agonist‐bound forms of G‐protein‐coupled receptors (GPCRs). This strict specificity is due at least partly to activation of GRK2 by agonist‐bound GPCRs, in which basic residues in intracellular regions adjacent to transmembrane segments are thought to be involved. Tubulin was found to be phosphorylated by GRK2, but it remains unknown if tubulin can also serve as both a substrate and an activator for GRK2. Purified tubulin, phosphorylated by GRK2, was subjected to biochemical analysis, and the phosphorylation sites in β‐tubulin were determined to be Thr409 and Ser420. In addition, the Ser444 in β III ‐tubulin was also indicated to be phosphorylated by GRK2. The phosphorylation sites in tubulin for GRK2 reside in the C‐terminal domain of β‐tubulin, which is on the outer surface of microtubules. Pretreatment of tubulin with protein phosphatase type‐2A (PP2A) resulted in a twofold increase in the phosphorylation of tubulin by GRK2. These results suggest that tubulin is phosphorylated in situ probably by GRK2 and that the phosphorylation may affect the interaction of microtubules with microtubule‐associated proteins. A GST fusion protein of a C‐terminal region of β I ‐tubulin (393–445 residues), containing 19 acidic residues but only one basic residue, was found to be a good substrate for GRK2, like full‐length β‐tubulin. These results, together with the finding that GRK2 may phosphorylate synuclein and phosducin in their acidic domains, indicate that some proteins with very acidic regions but without basic activation domains could serve as substrates for GRK2." @default.
• W2034157156 created "2016-06-24" @default.
• W2034157156 creator A5000523518 @default.
• W2034157156 creator A5006335559 @default.
• W2034157156 creator A5065554032 @default.
• W2034157156 date "2003-02-24" @default.
• W2034157156 modified "2023-10-17" @default.
• W2034157156 title "Identification of sites of phosphorylation by G-protein-coupled receptor kinase 2 in beta-tubulin" @default.
• W2034157156 cites W1033010459 @default.
• W2034157156 cites W1485119817 @default.
• W2034157156 cites W1497519474 @default.
• W2034157156 cites W1503310668 @default.
• W2034157156 cites W1517879640 @default.
• W2034157156 cites W1517947547 @default.
• W2034157156 cites W1531303582 @default.
• W2034157156 cites W1532095908 @default.
• W2034157156 cites W1571793851 @default.
• W2034157156 cites W1585007738 @default.
• W2034157156 cites W1947321730 @default.
• W2034157156 cites W1966244686 @default.
• W2034157156 cites W1974361709 @default.
• W2034157156 cites W1975040221 @default.
• W2034157156 cites W1983401747 @default.
• W2034157156 cites W1989380585 @default.
• W2034157156 cites W1991337227 @default.
• W2034157156 cites W1991899689 @default.
• W2034157156 cites W1994345832 @default.
• W2034157156 cites W1995605077 @default.
• W2034157156 cites W1996283200 @default.
• W2034157156 cites W1999801438 @default.
• W2034157156 cites W2004134377 @default.
• W2034157156 cites W2006068009 @default.
• W2034157156 cites W2014636427 @default.
• W2034157156 cites W2016839351 @default.
• W2034157156 cites W2021962189 @default.
• W2034157156 cites W2032058654 @default.
• W2034157156 cites W2035503730 @default.
• W2034157156 cites W2050269111 @default.
• W2034157156 cites W2053320675 @default.
• W2034157156 cites W2068989347 @default.
• W2034157156 cites W2082803210 @default.
• W2034157156 cites W2086617442 @default.
• W2034157156 cites W2087886422 @default.
• W2034157156 cites W2089742764 @default.
• W2034157156 cites W2092532048 @default.
• W2034157156 cites W2097549326 @default.
• W2034157156 cites W2100837269 @default.
• W2034157156 cites W2101108802 @default.
• W2034157156 cites W2103590067 @default.
• W2034157156 cites W2105190089 @default.
• W2034157156 cites W2143071207 @default.
• W2034157156 cites W2469069652 @default.
• W2034157156 cites W2614496215 @default.
• W2034157156 doi "https://doi.org/10.1046/j.1432-1033.2003.03465.x" @default.
• W2034157156 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12631274" @default.
• W2034157156 hasPublicationYear "2003" @default.
• W2034157156 type Work @default.
• W2034157156 sameAs 2034157156 @default.
• W2034157156 citedByCount "36" @default.
• W2034157156 countsByYear W20341571562012 @default.
• W2034157156 countsByYear W20341571562013 @default.
• W2034157156 countsByYear W20341571562014 @default.
• W2034157156 countsByYear W20341571562015 @default.
• W2034157156 countsByYear W20341571562016 @default.
• W2034157156 countsByYear W20341571562017 @default.
• W2034157156 countsByYear W20341571562019 @default.
• W2034157156 crossrefType "journal-article" @default.
• W2034157156 hasAuthorship W2034157156A5000523518 @default.
• W2034157156 hasAuthorship W2034157156A5006335559 @default.
• W2034157156 hasAuthorship W2034157156A5065554032 @default.
• W2034157156 hasConcept C11960822 @default.
• W2034157156 hasConcept C135285700 @default.
• W2034157156 hasConcept C170493617 @default.
• W2034157156 hasConcept C184235292 @default.
• W2034157156 hasConcept C185592680 @default.
• W2034157156 hasConcept C20418707 @default.
• W2034157156 hasConcept C33235085 @default.
• W2034157156 hasConcept C55493867 @default.
• W2034157156 hasConcept C78976303 @default.
• W2034157156 hasConcept C84425145 @default.
• W2034157156 hasConcept C86803240 @default.
• W2034157156 hasConcept C87325107 @default.
• W2034157156 hasConcept C95444343 @default.
• W2034157156 hasConcept C97029542 @default.
• W2034157156 hasConceptScore W2034157156C11960822 @default.
• W2034157156 hasConceptScore W2034157156C135285700 @default.
• W2034157156 hasConceptScore W2034157156C170493617 @default.
• W2034157156 hasConceptScore W2034157156C184235292 @default.
• W2034157156 hasConceptScore W2034157156C185592680 @default.
• W2034157156 hasConceptScore W2034157156C20418707 @default.
• W2034157156 hasConceptScore W2034157156C33235085 @default.
• W2034157156 hasConceptScore W2034157156C55493867 @default.
• W2034157156 hasConceptScore W2034157156C78976303 @default.
• W2034157156 hasConceptScore W2034157156C84425145 @default.
• W2034157156 hasConceptScore W2034157156C86803240 @default.
• W2034157156 hasConceptScore W2034157156C87325107 @default.
• W2034157156 hasConceptScore W2034157156C95444343 @default.
• W2034157156 hasConceptScore W2034157156C97029542 @default.

• next