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• W2034174466 abstract "Acetamiprid is a widely-used neonicotinoid insecticide often detected in the environment. In this study, we aimed to isolate high-efficient acetamiprid-degrading strains and to investigate the biodegradation pathway of acetamiprid in such microorganisms. An acetamiprid-degrading strain D-2 was isolated from a wastewater-treatment pool in an acetamiprid-manufacturing factory. Strain D-2 was identified as Pigmentiphaga sp. based on its morphological, physiological, and biochemical properties, as well as 16S rRNA gene analysis. Strain D-2 was shown to be highly effective in biodegrading of acetamiprid, degrading 0.22 mM acetamiprid to a non-detectable level within 72 h in a liquid culture. This bacterial strain efficiently degraded acetamiprid at temperatures ranging from 30 °C to 45 °C and pH values ranging from 5 to 10. During the degradation of acetamiprid, three metabolites 1-(6-chloropyridin-3-yl)-N-methylmethanamine, N′-[(6-chloropyridin-3-yl)methyl]-N-methylacetamide, and N′-cyano-N-methyl-N-(pyridin-3-ylmethyl)ethanimidamide were detected and identified based on tandem mass spectrometry and liquid chromatography-mass spectrometry analysis. A dechlorinated metabolite from acetamiprid was identified for the first time and confirmed by the detection of chlorine ion release during degradation. Using this information, a partial biodegradation pathway of acetamiprid in strain Pigmentiphaga sp. D-2 was proposed." @default.
• W2034174466 created "2016-06-24" @default.
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• W2034174466 date "2013-11-01" @default.
• W2034174466 modified "2023-10-17" @default.
• W2034174466 title "Biodegradation of acetamiprid by Pigmentiphaga sp. D-2 and the degradation pathway" @default.
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• W2034174466 doi "https://doi.org/10.1016/j.ibiod.2013.03.038" @default.
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