FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034176003> ?p ?o ?g. }

• W2034176003 abstract "Abstract Background Recent evidence demonstrates that 14-3-3σ acts as a tumor suppressor gene inactivated by methylation of its 5' CpG islands in epithelial tumor cells, while remaining un-methylated in normal human epithelia. The methylation analysis of 14-3-3σ has been largely overlooked in melanoma. Methods The methylation status of 14-3-3σ CpG island in melanocytes and melanoma cells was analyzed by methylation-specific sequencing (MSS) and quantitative methylation-specific PCR (Q-MSP). 14-3-3σ mRNA and protein expression in cell lines was detected by real-time RT-PCR and western blot. Melanoma cells were also treated by 5-aza-2'-deoxycytidine (DAC), a demethylating agent, and/or histone deacetylase inhibitor, Trichostatin A (TSA), to evaluate their effects on 14-3-3σ gene expression. Results 14-3-3σ is hypermethylated in both human melanocytes and most melanoma cells in a lineage-specific manner, resulting in the silencing of 14-3-3σ gene expression and the active induction of 14-3-3σ mRNA and protein expression following treatment with DAC. We also observed a synergistic effect upon gene expression when DAC was combined with TSA. The promoter methylation status of 14-3-3σ was analyzed utilizing Q-MSP in 20 melanoma tissue samples and 10 cell lines derived from these samples, showing that the majority of melanoma samples maintain their hypermethylation status of the 14-3-3σ gene. Conclusion 14-3-3σ is hypermethylated in human melanoma in a cell-linage specific manner. Spontaneous demethylation and re-expression of 14-3-3σ is a rare event in melanoma, indicating 14-3-3σ might have a tentative role in the pathogenesis of melanoma." @default.
• W2034176003 created "2016-06-24" @default.
• W2034176003 creator A5016049048 @default.
• W2034176003 creator A5030845183 @default.
• W2034176003 creator A5041564292 @default.
• W2034176003 creator A5063883059 @default.
• W2034176003 creator A5087841432 @default.
• W2034176003 date "2009-05-27" @default.
• W2034176003 modified "2023-09-26" @default.
• W2034176003 title "The 14-3-3σ gene promoter is methylated in both human melanocytes and melanoma" @default.
• W2034176003 cites W1967255508 @default.
• W2034176003 cites W1967785650 @default.
• W2034176003 cites W1980757374 @default.
• W2034176003 cites W1987760376 @default.
• W2034176003 cites W1994465394 @default.
• W2034176003 cites W2009983545 @default.
• W2034176003 cites W2014214643 @default.
• W2034176003 cites W2048408428 @default.
• W2034176003 cites W2063394028 @default.
• W2034176003 cites W2068880845 @default.
• W2034176003 cites W2087083968 @default.
• W2034176003 cites W2116646376 @default.
• W2034176003 cites W2120436117 @default.
• W2034176003 cites W2140009101 @default.
• W2034176003 cites W2140787953 @default.
• W2034176003 cites W2283944990 @default.
• W2034176003 cites W2332695910 @default.
• W2034176003 doi "https://doi.org/10.1186/1471-2407-9-162" @default.
• W2034176003 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2695475" @default.
• W2034176003 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19473536" @default.
• W2034176003 hasPublicationYear "2009" @default.
• W2034176003 type Work @default.
• W2034176003 sameAs 2034176003 @default.
• W2034176003 citedByCount "8" @default.
• W2034176003 countsByYear W20341760032012 @default.
• W2034176003 countsByYear W20341760032013 @default.
• W2034176003 countsByYear W20341760032014 @default.
• W2034176003 countsByYear W20341760032016 @default.
• W2034176003 countsByYear W20341760032022 @default.
• W2034176003 crossrefType "journal-article" @default.
• W2034176003 hasAuthorship W2034176003A5016049048 @default.
• W2034176003 hasAuthorship W2034176003A5030845183 @default.
• W2034176003 hasAuthorship W2034176003A5041564292 @default.
• W2034176003 hasAuthorship W2034176003A5063883059 @default.
• W2034176003 hasAuthorship W2034176003A5087841432 @default.
• W2034176003 hasBestOaLocation W20341760031 @default.
• W2034176003 hasConcept C104317684 @default.
• W2034176003 hasConcept C119056186 @default.
• W2034176003 hasConcept C140173407 @default.
• W2034176003 hasConcept C150194340 @default.
• W2034176003 hasConcept C153911025 @default.
• W2034176003 hasConcept C189235521 @default.
• W2034176003 hasConcept C190727270 @default.
• W2034176003 hasConcept C2777658100 @default.
• W2034176003 hasConcept C2777865847 @default.
• W2034176003 hasConcept C2778305200 @default.
• W2034176003 hasConcept C2779277951 @default.
• W2034176003 hasConcept C33288867 @default.
• W2034176003 hasConcept C41091548 @default.
• W2034176003 hasConcept C502942594 @default.
• W2034176003 hasConcept C54355233 @default.
• W2034176003 hasConcept C64927066 @default.
• W2034176003 hasConcept C86803240 @default.
• W2034176003 hasConceptScore W2034176003C104317684 @default.
• W2034176003 hasConceptScore W2034176003C119056186 @default.
• W2034176003 hasConceptScore W2034176003C140173407 @default.
• W2034176003 hasConceptScore W2034176003C150194340 @default.
• W2034176003 hasConceptScore W2034176003C153911025 @default.
• W2034176003 hasConceptScore W2034176003C189235521 @default.
• W2034176003 hasConceptScore W2034176003C190727270 @default.
• W2034176003 hasConceptScore W2034176003C2777658100 @default.
• W2034176003 hasConceptScore W2034176003C2777865847 @default.
• W2034176003 hasConceptScore W2034176003C2778305200 @default.
• W2034176003 hasConceptScore W2034176003C2779277951 @default.
• W2034176003 hasConceptScore W2034176003C33288867 @default.
• W2034176003 hasConceptScore W2034176003C41091548 @default.
• W2034176003 hasConceptScore W2034176003C502942594 @default.
• W2034176003 hasConceptScore W2034176003C54355233 @default.
• W2034176003 hasConceptScore W2034176003C64927066 @default.
• W2034176003 hasConceptScore W2034176003C86803240 @default.
• W2034176003 hasIssue "1" @default.
• W2034176003 hasLocation W20341760031 @default.
• W2034176003 hasLocation W20341760032 @default.
• W2034176003 hasLocation W20341760033 @default.
• W2034176003 hasLocation W20341760034 @default.
• W2034176003 hasOpenAccess W2034176003 @default.
• W2034176003 hasPrimaryLocation W20341760031 @default.
• W2034176003 hasRelatedWork W1519915707 @default.
• W2034176003 hasRelatedWork W1862483845 @default.
• W2034176003 hasRelatedWork W1983565617 @default.
• W2034176003 hasRelatedWork W2000878529 @default.
• W2034176003 hasRelatedWork W2013698368 @default.
• W2034176003 hasRelatedWork W2055695109 @default.
• W2034176003 hasRelatedWork W2071539014 @default.
• W2034176003 hasRelatedWork W3174211233 @default.
• W2034176003 hasRelatedWork W4290860628 @default.
• W2034176003 hasRelatedWork W779414787 @default.
• W2034176003 hasVolume "9" @default.
• W2034176003 isParatext "false" @default.
• W2034176003 isRetracted "false" @default.

• next