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• W2034178689 abstract "It was shown previously [(1984) EMBO J. 3, 453‐459] that after treatment of the human erythroleukemia cell line HEL with phorbol ester and dimethyl sulfoxide there was a marked increase in the amounts of megakaryotic markers, especially of platelet α‐granule proteins and platelet glycoproteins. In order to investigate this differentiation process further we have studied the expression of the mRNA encoding PDGF‐A and PDGF‐B (c‐sis). Upon addition of the phorbol ester to the culture medium the expression of the c‐sis transcript was enhanced about 7‐fold over a period of 4 days. With dimethyl sulfoxide there was no significant stimulation of the expression. Addition of cycloheximide to HEL cells treated for a short period with phorbol ester superinduced the expression of the c‐sis gene. The HEL cells did not express the A‐chain mRNA even in the presence of phorbol ester or dimethyl sulfoxide. This leads us to propose that synthesis of the PDGF‐A chain and PDGF‐B chain is differentially regulated in the megakaryocytic‐like HEL cell line." @default.
• W2034178689 created "2016-06-24" @default.
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• W2034178689 date "1987-03-09" @default.
• W2034178689 modified "2023-09-26" @default.
• W2034178689 title "Platelet-derived growth factor Phorbol ester induces the expression of the B-chain but not of the A-chain in HEL cells" @default.
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• W2034178689 doi "https://doi.org/10.1016/0014-5793(87)81470-9" @default.
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