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- W2034180246 abstract "Abstract Thymine-starvation was found to be mutagenic for thymine-requiring mutants of Salmonella typhimurium LT2. Thymine auxotrophs were thymine-starved to a level of 10% survival. Readdition of thymine and other nutrients, followed by selective techniques, resulted in the recovery of cells possessing additional auxotrophic mutations. A set of 70 mutants was collected and tested for reversion to phototrophy both spontaneously and after chemical mutagen treatment. Reversion tests of these mutants revealed that revertible (spontaneously and by mutagen), non-revertible (spontaneously nor by mutagen), and mutagen-stable (spontaneously revertible, but not by mutagen) mutants were induced by thymineless treatment. Furthermore, the revertible mutants were not identical to each other with regard to reversion pattern when tested with a series of mutagens. It is concluded from these results that mutation by thymine-starvation is not limited to base-pair substitution. Mutants isolated after thymine-starvation were actually induced and not merely selected from mutants pre-existing in the cell population. This conclusion was based on reconstruction experiments and on comparisons of selectivity and mutability between thymine-starvation and penicillin-screening." @default.
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- W2034180246 date "1968-01-01" @default.
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- W2034180246 title "The mutagenic effect of thymine-starvation on Salmonella typhimurium" @default.
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- W2034180246 doi "https://doi.org/10.1016/0027-5107(68)90076-6" @default.
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