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- W2034185497 abstract "Incorporation of [ 14 C]valine into protein with an amino acid incorporation system from Salmonella typhimurium is shown to be stimulated by the addition of T2 phage DNA plus purified RNA polymerase or by T2 RNA prepared with the polymerase. T2 RNA isolated by several procedures is active in stimulating [ 14 C]valine incorporation into protein and, in contrast to the DNA stimulation, the effect is not dependent on addition of RNA polymerase or affected by DNase addition. The stimulation by T2 RNA is considerably less than that of DNA plus the polymerase. The stimulatory T2 RNA shows heterogeneous sedimentation properties, as determined by sucrose density gradient centrifugation, and, on the average, sediments slightly faster than ribosomal RNA. DEAE cellulose column chromatography of labeled soluble protein formed with a 30,000 g supernatant fraction of Salmonella typhimurium suggests formation of a different protein or proteins on addition of T2 DNA." @default.
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- W2034185497 date "1962-12-01" @default.
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- W2034185497 title "Studies of the effect of T2 RNA formed with purified RNA polymerase on amino acid incorporation into protein" @default.
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- W2034185497 doi "https://doi.org/10.1016/s0022-2836(62)80093-x" @default.
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