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- W2034187893 abstract "Whole-cell patch neurophysiology and pharmacological manipulations have provided unprecedented insight into the functions of central neurons, but their combined use has been largely restricted to in vitro preparations. We describe a method for performing whole-cell patch recording and focal application of pharmacological agents in vivo. A key feature of this technique involves iontophoresis of glutamate to establish proximity of drug and recording pipettes. We show data from iontophoresis of glutamate during extracellular and whole-cell recordings made in vivo from auditory neurons in the midbrain of the leopard frog, Rana pipiens, and the effects of blocking GABA(A) receptors while making a whole-cell recording. This methodology should accelerate our understanding of the roles of particular neurotransmitter systems in normal and pathological conditions, and facilitate investigation of the in vivo effects of drugs and the mechanisms underlying computations." @default.
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- W2034187893 date "2013-02-01" @default.
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- W2034187893 title "Combining pharmacology and whole-cell patch recording from CNS neurons, in vivo" @default.
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- W2034187893 doi "https://doi.org/10.1016/j.jneumeth.2012.12.003" @default.
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