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• W2034190821 abstract "Cdc42, a member of the Rho GTPase family, is a fundamental regulator of the actin cytoskeleton during cell migration. To generate a sensor for Cdc42 activation, we employed a multi-pronged approach, utilizing cysteine labeling and expressed protein ligation, to incorporate the environment sensitive fluorophore 4-N,N-dimethylamino-1,8-naphthalimide (4-DMN) into the GTPase binding domain of the WASP protein. These constructs bind only the active, GTP-bound conformation of Cdc42 to produce a fluorescence signal. Studies with a panel of five sensor analogs revealed a derivative that exhibits a 32-fold increase in fluorescence intensity in the presence of activated Cdc42 compared to incubation with the inactive GDP-bound form of the protein. We demonstrate that this sensor can be exploited to monitor Cdc42 nucleotide exchange and GTPase activity in a continuous, fluorescence assay." @default.
• W2034190821 created "2016-06-24" @default.
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• W2034190821 date "2011-09-01" @default.
• W2034190821 modified "2023-09-23" @default.
• W2034190821 title "Development of a fluorogenic sensor for activated Cdc42" @default.
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• W2034190821 doi "https://doi.org/10.1016/j.bmcl.2011.04.051" @default.
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