FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034195216> ?p ?o ?g. }

• W2034195216 endingPage "733" @default.
• W2034195216 startingPage "723" @default.
• W2034195216 abstract "Covalent modification of skin proteins by electrophiles is a key event in the induction of skin sensitisation but not skin irritation although the exact nature of the binding mechanisms has not been determined empirically for the vast majority of sensitisers. It is also unknown whether immunologically relevant protein targets exist in the skin contributing to effecting skin sensitisation. To determine the haptenation mechanism(s) and spectra of amino acid reactivity in an intact protein for two sensitisers expected to react by different mechanisms, human serum albumin (HSA) was chosen as a model protein. The aim of this work was also to verify for selected non-sensitisers and irritants that no protein haptenation occurs even under forcing conditions. HSA was incubated with chemicals and the resulting complexes were digested with trypsin and analysed deploying matrix-assisted laser desorption/ionization mass spectrometry, reverse phase high performance liquid chromatography and nano-electrospray tandem mass spectrometry. The data confirmed that different residues (lysine, cysteine, histidine and tyrosine) are covalently modified in a highly selective and differential manner by the sensitisers 2,4-dinitro-1-chlorobenzene and phenyl salicylate. Additionally, non-sensitisers 2,4-dichloro-1-nitrobenzene, butyl paraben and benzaldehyde and irritants benzalkonium chloride and sodium dodecyl sulphate did not covalently modify HSA under any conditions. The data indicate that covalent haptenation is a prerequisite of skin sensitisation but not irritation. The data also suggest that protein modifications are targeted to certain amino acids residing in chemical microenvironments conducive to reactivity within an intact protein. Deriving such information is relevant to our understanding of antigen formation in the immunobiology of skin sensitisation and in the development of in vitro protein haptenation assays." @default.
• W2034195216 created "2016-06-24" @default.
• W2034195216 creator A5006940529 @default.
• W2034195216 creator A5017214719 @default.
• W2034195216 creator A5019104680 @default.
• W2034195216 creator A5026306331 @default.
• W2034195216 creator A5080001443 @default.
• W2034195216 creator A5081887234 @default.
• W2034195216 date "2007-06-01" @default.
• W2034195216 modified "2023-09-26" @default.
• W2034195216 title "Investigating protein haptenation mechanisms of skin sensitisers using human serum albumin as a model protein" @default.
• W2034195216 cites W1504792186 @default.
• W2034195216 cites W1570348606 @default.
• W2034195216 cites W1575563617 @default.
• W2034195216 cites W1968506788 @default.
• W2034195216 cites W1981792256 @default.
• W2034195216 cites W1988600195 @default.
• W2034195216 cites W1993473403 @default.
• W2034195216 cites W1994270945 @default.
• W2034195216 cites W1994342083 @default.
• W2034195216 cites W1996948109 @default.
• W2034195216 cites W2000062025 @default.
• W2034195216 cites W2006297498 @default.
• W2034195216 cites W2010833696 @default.
• W2034195216 cites W2021817496 @default.
• W2034195216 cites W2022648163 @default.
• W2034195216 cites W2025391005 @default.
• W2034195216 cites W2038258213 @default.
• W2034195216 cites W2041755489 @default.
• W2034195216 cites W2041795762 @default.
• W2034195216 cites W2041825373 @default.
• W2034195216 cites W2045561638 @default.
• W2034195216 cites W2049071218 @default.
• W2034195216 cites W2054663215 @default.
• W2034195216 cites W2060416187 @default.
• W2034195216 cites W2064336019 @default.
• W2034195216 cites W2075185078 @default.
• W2034195216 cites W2075382621 @default.
• W2034195216 cites W2081159973 @default.
• W2034195216 cites W2082293043 @default.
• W2034195216 cites W2084377056 @default.
• W2034195216 cites W2098669559 @default.
• W2034195216 cites W2107543992 @default.
• W2034195216 cites W2110108389 @default.
• W2034195216 cites W2117809365 @default.
• W2034195216 cites W2141486311 @default.
• W2034195216 cites W2157971131 @default.
• W2034195216 cites W2164496815 @default.
• W2034195216 cites W2318267881 @default.
• W2034195216 doi "https://doi.org/10.1016/j.tiv.2007.01.008" @default.
• W2034195216 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17317089" @default.
• W2034195216 hasPublicationYear "2007" @default.
• W2034195216 type Work @default.
• W2034195216 sameAs 2034195216 @default.
• W2034195216 citedByCount "63" @default.
• W2034195216 countsByYear W20341952162012 @default.
• W2034195216 countsByYear W20341952162013 @default.
• W2034195216 countsByYear W20341952162014 @default.
• W2034195216 countsByYear W20341952162015 @default.
• W2034195216 countsByYear W20341952162016 @default.
• W2034195216 countsByYear W20341952162017 @default.
• W2034195216 countsByYear W20341952162018 @default.
• W2034195216 countsByYear W20341952162019 @default.
• W2034195216 countsByYear W20341952162020 @default.
• W2034195216 countsByYear W20341952162022 @default.
• W2034195216 countsByYear W20341952162023 @default.
• W2034195216 crossrefType "journal-article" @default.
• W2034195216 hasAuthorship W2034195216A5006940529 @default.
• W2034195216 hasAuthorship W2034195216A5017214719 @default.
• W2034195216 hasAuthorship W2034195216A5019104680 @default.
• W2034195216 hasAuthorship W2034195216A5026306331 @default.
• W2034195216 hasAuthorship W2034195216A5080001443 @default.
• W2034195216 hasAuthorship W2034195216A5081887234 @default.
• W2034195216 hasConcept C178790620 @default.
• W2034195216 hasConcept C180577832 @default.
• W2034195216 hasConcept C181199279 @default.
• W2034195216 hasConcept C185592680 @default.
• W2034195216 hasConcept C2778460671 @default.
• W2034195216 hasConcept C2778597219 @default.
• W2034195216 hasConcept C2780340462 @default.
• W2034195216 hasConcept C43617362 @default.
• W2034195216 hasConcept C515207424 @default.
• W2034195216 hasConcept C55493867 @default.
• W2034195216 hasConceptScore W2034195216C178790620 @default.
• W2034195216 hasConceptScore W2034195216C180577832 @default.
• W2034195216 hasConceptScore W2034195216C181199279 @default.
• W2034195216 hasConceptScore W2034195216C185592680 @default.
• W2034195216 hasConceptScore W2034195216C2778460671 @default.
• W2034195216 hasConceptScore W2034195216C2778597219 @default.
• W2034195216 hasConceptScore W2034195216C2780340462 @default.
• W2034195216 hasConceptScore W2034195216C43617362 @default.
• W2034195216 hasConceptScore W2034195216C515207424 @default.
• W2034195216 hasConceptScore W2034195216C55493867 @default.
• W2034195216 hasIssue "4" @default.
• W2034195216 hasLocation W20341952161 @default.
• W2034195216 hasLocation W20341952162 @default.
• W2034195216 hasOpenAccess W2034195216 @default.
• W2034195216 hasPrimaryLocation W20341952161 @default.

• next