Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034198717> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W2034198717 endingPage "81" @default.
- W2034198717 startingPage "71" @default.
- W2034198717 abstract "The reference method for detecting specific Epstein-Barr virus (EBV) antibodies is indirect immunofluorescence (IF) with EBV-infected cells. The availability of protein purified from infected cells and more recently of recombinant polypeptides designed to contain immunodominant epitopes, has enabled the development of commercial enzyme-linked immunosorbent assays (ELISA) for the specific serodiagnosis of EBV infection.Evaluation of ELISA-based EBV serodiagnosis in comparison with indirect immunofluorescence.We have first compared three commercial ELISA test systems with our in house indirect immunofluorescence assay for classifying correctly a set of serum samples into clinical categories (acute infection, past infection, interfering non-EBV infection, persistent infection). Additionally a prospective analysis with the best performing ELISA test (Enzygnost) was then carried out by running the ELISA test in parallel with the indirect immunofluorescence assay on 324 consecutive clinical samples sent to our laboratory for EBV serodiagnosis.For the serodiagnosis of past EBV infection and acute EBV infection all three commercial ELISAs performed well in comparison with indirect immunofluorescence. When testing samples positive for cytomegalovirus (CMV), Toxoplasma or herpes simplex IgM, interference in the IgM tests was observed with the three ELISAs. In some instances we could demonstrate that the positive IgM results were due to EBV reactivation. The observed discrepancies between ELISA and IF for the serodiagnosis of chronic EBV infection or EBV reactivation, point to the difficulty for the serodiagnosis of persistent EBV infection on single serum samples. According to our prospective study the EBV IgG determination was accurate. A positive IgM result was not always indicative of an acute infection. Positive IgM results due to EBV reactivation were observed. A positive EBV nuclear antigen (EBNA) IgG result in those samples precluded acute infection.90-95% of samples could be classified correctly into clinical categories by a two parameter ELISA system detecting IgG and IgM against a standardized mixture of EBV antigens, allowing standardization and automation of EBV-specific serology. The absence of EBNA IgG was useful as a second line confirmatory assay for acute EBV infection." @default.
- W2034198717 created "2016-06-24" @default.
- W2034198717 creator A5019719611 @default.
- W2034198717 creator A5040268097 @default.
- W2034198717 creator A5044112097 @default.
- W2034198717 creator A5088355541 @default.
- W2034198717 date "1997-05-01" @default.
- W2034198717 modified "2023-09-24" @default.
- W2034198717 title "Comparative evaluation of three ELISA techniques and an indirect immunofluorescence assay for the serological diagnosis of Epstein-Barr virus infection" @default.
- W2034198717 cites W1520981706 @default.
- W2034198717 cites W1630076302 @default.
- W2034198717 cites W1973221935 @default.
- W2034198717 cites W2013604623 @default.
- W2034198717 cites W2020956100 @default.
- W2034198717 cites W2060073761 @default.
- W2034198717 cites W2092719806 @default.
- W2034198717 cites W2171915728 @default.
- W2034198717 cites W2331570623 @default.
- W2034198717 doi "https://doi.org/10.1016/s0928-0197(97)00014-7" @default.
- W2034198717 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9248660" @default.
- W2034198717 hasPublicationYear "1997" @default.
- W2034198717 type Work @default.
- W2034198717 sameAs 2034198717 @default.
- W2034198717 citedByCount "33" @default.
- W2034198717 countsByYear W20341987172012 @default.
- W2034198717 countsByYear W20341987172013 @default.
- W2034198717 countsByYear W20341987172014 @default.
- W2034198717 countsByYear W20341987172015 @default.
- W2034198717 countsByYear W20341987172016 @default.
- W2034198717 countsByYear W20341987172018 @default.
- W2034198717 crossrefType "journal-article" @default.
- W2034198717 hasAuthorship W2034198717A5019719611 @default.
- W2034198717 hasAuthorship W2034198717A5040268097 @default.
- W2034198717 hasAuthorship W2034198717A5044112097 @default.
- W2034198717 hasAuthorship W2034198717A5088355541 @default.
- W2034198717 hasConcept C159047783 @default.
- W2034198717 hasConcept C159654299 @default.
- W2034198717 hasConcept C203014093 @default.
- W2034198717 hasConcept C2522874641 @default.
- W2034198717 hasConcept C2778236600 @default.
- W2034198717 hasConcept C2780494569 @default.
- W2034198717 hasConcept C2781375147 @default.
- W2034198717 hasConcept C2984894117 @default.
- W2034198717 hasConcept C45189115 @default.
- W2034198717 hasConcept C86803240 @default.
- W2034198717 hasConceptScore W2034198717C159047783 @default.
- W2034198717 hasConceptScore W2034198717C159654299 @default.
- W2034198717 hasConceptScore W2034198717C203014093 @default.
- W2034198717 hasConceptScore W2034198717C2522874641 @default.
- W2034198717 hasConceptScore W2034198717C2778236600 @default.
- W2034198717 hasConceptScore W2034198717C2780494569 @default.
- W2034198717 hasConceptScore W2034198717C2781375147 @default.
- W2034198717 hasConceptScore W2034198717C2984894117 @default.
- W2034198717 hasConceptScore W2034198717C45189115 @default.
- W2034198717 hasConceptScore W2034198717C86803240 @default.
- W2034198717 hasIssue "1" @default.
- W2034198717 hasLocation W20341987171 @default.
- W2034198717 hasLocation W20341987172 @default.
- W2034198717 hasOpenAccess W2034198717 @default.
- W2034198717 hasPrimaryLocation W20341987171 @default.
- W2034198717 hasRelatedWork W1029846521 @default.
- W2034198717 hasRelatedWork W159384773 @default.
- W2034198717 hasRelatedWork W1999539361 @default.
- W2034198717 hasRelatedWork W2017428364 @default.
- W2034198717 hasRelatedWork W2025049706 @default.
- W2034198717 hasRelatedWork W2030238129 @default.
- W2034198717 hasRelatedWork W2034198717 @default.
- W2034198717 hasRelatedWork W2088083815 @default.
- W2034198717 hasRelatedWork W2130724711 @default.
- W2034198717 hasRelatedWork W2402288543 @default.
- W2034198717 hasVolume "8" @default.
- W2034198717 isParatext "false" @default.
- W2034198717 isRetracted "false" @default.
- W2034198717 magId "2034198717" @default.
- W2034198717 workType "article" @default.