FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034200992> ?p ?o ?g. }

• W2034200992 endingPage "1132" @default.
• W2034200992 startingPage "1126" @default.
• W2034200992 abstract "Commercial gel electrophoresis apparatus with intermittent fluorescence scanning of the migration path (HPGE-1000 apparatus, LabIntelligence) makes it possible to measure band width and migration distance as a function of the duration of electrophoresis. As a result, resolution can be evaluated quantitatively and therefore different gel media can be compared objectively. The resolution of fluorescein carboxylate labeled conalbumin (molecular mass 86 kDa) and soybean trypsin inhibitor (22.7 kDa) in gel electrophoresis was found to increase as a function of the gel type in the order SeaKem GTG-, SeaKem Gold-agarose, 2% N,N′-methylenebisacrylamide cross-linked polyacrylamide, MetaPhor-XR-, and SeaPrep-agarose. The advantage in resolving capacity of SeaPrep agarose over the polyacrylamide gel was by a factor of up to five. The resolving capacity of the agaroses was in indirect relation to the degree of electroendosmosis. In all media, resolution increased with migration distance (time). The same proteins when reacted with sodium dodecyl sulfate (SDS) resolve (i) better at up to 6% SeaPrep agarose concentration than in polyacrylamide, as in the gel electrophoresis of the native proteins; (ii) less effectively, by contrast, at SeaPrep agarose concentrations > 6%, than in polyacrylamide gel; and (iii) significantly better in 4–6% SeaPrep agarose than in 4–6% SeaKem GTG agarose. Since Ferguson plot analysis in both agarose and polyacrylamide gels shows that the two SDS-proteins are larger than the native proteins with which they are complexed, the superiority of polyacrylamide gels above 7% appears to be correlated with the fact that its mean pore radius, estimated for both media using identical assumptions and identical rigid spherical standards — proteins, is approximately seven times larger than that of SeaPrep agarose in the concentration range of 3–8%, and that therefore the molecular “fit” in polyacrylamide is closer than that in SeaPrep agarose of the concentration range used. The dependence of resolution on the ratio of particle radius to mean pore radius (“fit”) is also suggested by the fact that the two SDS-proteins resolve in a biphasic dependence on gel concentration in both agarose and polyacrylamide, with a maximum at 6% agarose and 10% polyacrylamide." @default.
• W2034200992 created "2016-06-24" @default.
• W2034200992 creator A5017630299 @default.
• W2034200992 creator A5064963337 @default.
• W2034200992 date "1997-01-01" @default.
• W2034200992 modified "2023-09-25" @default.
• W2034200992 title "The resolution between two native proteins and between their sodium dodecyl sulfate-complexes in agarose and polyacrylamide gel electrophoresis" @default.
• W2034200992 cites W1966737442 @default.
• W2034200992 cites W1985816589 @default.
• W2034200992 cites W2007943281 @default.
• W2034200992 cites W2015273523 @default.
• W2034200992 cites W2023175261 @default.
• W2034200992 cites W2023887656 @default.
• W2034200992 cites W2047204005 @default.
• W2034200992 cites W2047903470 @default.
• W2034200992 cites W2064027299 @default.
• W2034200992 cites W2073481159 @default.
• W2034200992 cites W2074041522 @default.
• W2034200992 cites W2079491729 @default.
• W2034200992 cites W2092796859 @default.
• W2034200992 cites W2106904194 @default.
• W2034200992 cites W2107295946 @default.
• W2034200992 cites W4238181268 @default.
• W2034200992 doi "https://doi.org/10.1002/elps.1150180717" @default.
• W2034200992 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9237567" @default.
• W2034200992 hasPublicationYear "1997" @default.
• W2034200992 type Work @default.
• W2034200992 sameAs 2034200992 @default.
• W2034200992 citedByCount "12" @default.
• W2034200992 countsByYear W20342009922023 @default.
• W2034200992 crossrefType "journal-article" @default.
• W2034200992 hasAuthorship W2034200992A5017630299 @default.
• W2034200992 hasAuthorship W2034200992A5064963337 @default.
• W2034200992 hasConcept C101660505 @default.
• W2034200992 hasConcept C116084860 @default.
• W2034200992 hasConcept C131506403 @default.
• W2034200992 hasConcept C152862880 @default.
• W2034200992 hasConcept C181199279 @default.
• W2034200992 hasConcept C185592680 @default.
• W2034200992 hasConcept C188027245 @default.
• W2034200992 hasConcept C190911170 @default.
• W2034200992 hasConcept C2778271344 @default.
• W2034200992 hasConcept C2778476535 @default.
• W2034200992 hasConcept C2778862431 @default.
• W2034200992 hasConcept C2778913249 @default.
• W2034200992 hasConcept C35859425 @default.
• W2034200992 hasConcept C40250595 @default.
• W2034200992 hasConcept C43617362 @default.
• W2034200992 hasConcept C552990157 @default.
• W2034200992 hasConcept C55493867 @default.
• W2034200992 hasConceptScore W2034200992C101660505 @default.
• W2034200992 hasConceptScore W2034200992C116084860 @default.
• W2034200992 hasConceptScore W2034200992C131506403 @default.
• W2034200992 hasConceptScore W2034200992C152862880 @default.
• W2034200992 hasConceptScore W2034200992C181199279 @default.
• W2034200992 hasConceptScore W2034200992C185592680 @default.
• W2034200992 hasConceptScore W2034200992C188027245 @default.
• W2034200992 hasConceptScore W2034200992C190911170 @default.
• W2034200992 hasConceptScore W2034200992C2778271344 @default.
• W2034200992 hasConceptScore W2034200992C2778476535 @default.
• W2034200992 hasConceptScore W2034200992C2778862431 @default.
• W2034200992 hasConceptScore W2034200992C2778913249 @default.
• W2034200992 hasConceptScore W2034200992C35859425 @default.
• W2034200992 hasConceptScore W2034200992C40250595 @default.
• W2034200992 hasConceptScore W2034200992C43617362 @default.
• W2034200992 hasConceptScore W2034200992C552990157 @default.
• W2034200992 hasConceptScore W2034200992C55493867 @default.
• W2034200992 hasIssue "7" @default.
• W2034200992 hasLocation W20342009921 @default.
• W2034200992 hasLocation W20342009922 @default.
• W2034200992 hasOpenAccess W2034200992 @default.
• W2034200992 hasPrimaryLocation W20342009921 @default.
• W2034200992 hasRelatedWork W1996982069 @default.
• W2034200992 hasRelatedWork W1997557332 @default.
• W2034200992 hasRelatedWork W1999076623 @default.
• W2034200992 hasRelatedWork W2007419324 @default.
• W2034200992 hasRelatedWork W2034200992 @default.
• W2034200992 hasRelatedWork W2067760590 @default.
• W2034200992 hasRelatedWork W2091718956 @default.
• W2034200992 hasRelatedWork W2141055395 @default.
• W2034200992 hasRelatedWork W4210410859 @default.
• W2034200992 hasRelatedWork W1991253447 @default.
• W2034200992 hasVolume "18" @default.
• W2034200992 isParatext "false" @default.
• W2034200992 isRetracted "false" @default.
• W2034200992 magId "2034200992" @default.
• W2034200992 workType "article" @default.