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- W2034201039 abstract "Abstract A monoclonal antibody directed against the adenovirus type 5 Elb-58Kd tumor antigen has been isolated. Immune clearing experiments were employed to demonstrate that this monoclonal antibody detects the same Elb-58Kd antigen observed previously with polyclonal antisera from hamsters bearing adenovirus-induced tumors. Fluorescent antibody tests using the monoclonal antibodies directed against the Elb-58Kd antigen localized the viral protein predominantly in the nucleus of virus-infected and -transformed cells. The monoclonal and polyclonal tumor sera detected similar protein species in virusinfected and -transformed cells. In adenovirus productively infected human cells the monoclonal antibody and polyclonal tumor sera both detected a 25Kd protein in addition to the Elb-58Kd antigen. In transformed mouse and hamster cells this monoclonal and polyclonal antisera coimmunoprecipitated a 53Kd cellular protein along with the Elb58Kd antigen. This 53Kd cellular protein is similar to the p53 antigens detected in a variety of transformed cells." @default.
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- W2034201039 title "A monoclonal antibody detecting the adenovirus type 5 E 1 b-58Kd tumor antigen: Characterization of the E 1 b-58Kd tumor antigen in adenovirus-infected and -transformed cells" @default.
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- W2034201039 doi "https://doi.org/10.1016/0042-6822(82)90054-x" @default.
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