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- W2034201571 abstract "ABSTRACT CRISPR-Cas systems provide adaptive microbial immunity against invading viruses and plasmids. The cariogenic bacterium Streptococcus mutans UA159 has two CRISPR-Cas systems: CRISPR1 (type II-A) and CRISPR2 (type I-C) with several spacers from both CRISPR cassettes matching sequences of phage M102 or genomic sequences of other S. mutans . The deletion of the cas genes of CRISPR1 (ΔC1S), CRISPR2 (ΔC2E), or both CRISPR1+2 (ΔC1SC2E) or the removal of spacers 2 and 3 (ΔCR1SP13E) in S. mutans UA159 did not affect phage sensitivity when challenged with virulent phage M102. Using plasmid transformation experiments, we demonstrated that the CRISPR1-Cas system inhibits transformation of S. mutans by the plasmids matching the spacers 2 and 3. Functional analysis of the cas deletion mutants revealed that in addition to a role in plasmid targeting, both CRISPR systems also contribute to the regulation of bacterial physiology in S. mutans . Compared to wild-type cells, the ΔC1S strain displayed diminished growth under cell membrane and oxidative stress, enhanced growth under low pH, and had reduced survival under heat shock and DNA-damaging conditions, whereas the ΔC2E strain exhibited increased sensitivity to heat shock. Transcriptional analysis revealed that the two-component signal transduction system VicR/K differentially modulates expression of cas genes within CRISPR-Cas systems, suggesting that VicR/K might coordinate the expression of two CRISPR-Cas systems. Collectively, we provide in vivo evidence that the type II-A CRISPR-Cas system of S. mutans may be targeted to manipulate its stress response and to influence the host to control the uptake and dissemination of antibiotic resistance genes." @default.
- W2034201571 created "2016-06-24" @default.
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- W2034201571 date "2015-02-15" @default.
- W2034201571 modified "2023-10-01" @default.
- W2034201571 title "Role of the Streptococcus mutans CRISPR-Cas Systems in Immunity and Cell Physiology" @default.
- W2034201571 cites W1499798127 @default.
- W2034201571 cites W1577973179 @default.
- W2034201571 cites W1894740506 @default.
- W2034201571 cites W1963651147 @default.
- W2034201571 cites W1965404910 @default.
- W2034201571 cites W1970515032 @default.
- W2034201571 cites W1970776621 @default.
- W2034201571 cites W1977531433 @default.
- W2034201571 cites W1977782657 @default.
- W2034201571 cites W1977969929 @default.
- W2034201571 cites W1978094899 @default.
- W2034201571 cites W1980760811 @default.
- W2034201571 cites W1986171241 @default.
- W2034201571 cites W1986718765 @default.
- W2034201571 cites W1989411585 @default.
- W2034201571 cites W1989568784 @default.
- W2034201571 cites W1990487336 @default.
- W2034201571 cites W2001487604 @default.
- W2034201571 cites W2004494938 @default.
- W2034201571 cites W2008790072 @default.
- W2034201571 cites W2009576434 @default.
- W2034201571 cites W2010187816 @default.
- W2034201571 cites W2010986636 @default.
- W2034201571 cites W2016992644 @default.
- W2034201571 cites W2017721099 @default.
- W2034201571 cites W2019670881 @default.
- W2034201571 cites W2027591298 @default.
- W2034201571 cites W2034048377 @default.
- W2034201571 cites W2038838144 @default.
- W2034201571 cites W2043610929 @default.
- W2034201571 cites W2045435533 @default.
- W2034201571 cites W2052192736 @default.
- W2034201571 cites W2053402985 @default.
- W2034201571 cites W2057092463 @default.
- W2034201571 cites W2057964816 @default.
- W2034201571 cites W2059363769 @default.
- W2034201571 cites W2063148079 @default.
- W2034201571 cites W2063376698 @default.
- W2034201571 cites W2065893609 @default.
- W2034201571 cites W2067169557 @default.
- W2034201571 cites W2067853474 @default.
- W2034201571 cites W2072051684 @default.
- W2034201571 cites W2074862753 @default.
- W2034201571 cites W2076785026 @default.
- W2034201571 cites W2095298897 @default.
- W2034201571 cites W2098508116 @default.
- W2034201571 cites W2101788140 @default.
- W2034201571 cites W2101983500 @default.
- W2034201571 cites W2102822311 @default.
- W2034201571 cites W2103006246 @default.
- W2034201571 cites W2106900261 @default.
- W2034201571 cites W2108244474 @default.
- W2034201571 cites W2108429999 @default.
- W2034201571 cites W2108598630 @default.
- W2034201571 cites W2112046950 @default.
- W2034201571 cites W2112295554 @default.
- W2034201571 cites W2112671289 @default.
- W2034201571 cites W2118937626 @default.
- W2034201571 cites W2120503144 @default.
- W2034201571 cites W2123965484 @default.
- W2034201571 cites W2126281022 @default.
- W2034201571 cites W2128301757 @default.
- W2034201571 cites W2129145003 @default.
- W2034201571 cites W2130266421 @default.
- W2034201571 cites W2130916371 @default.
- W2034201571 cites W2131527797 @default.
- W2034201571 cites W2135598273 @default.
- W2034201571 cites W2137999973 @default.
- W2034201571 cites W2139580946 @default.
- W2034201571 cites W2140576558 @default.
- W2034201571 cites W2143554177 @default.
- W2034201571 cites W2144388403 @default.
- W2034201571 cites W2144566978 @default.
- W2034201571 cites W2147073037 @default.
- W2034201571 cites W2149751339 @default.
- W2034201571 cites W2150927648 @default.
- W2034201571 cites W2151378885 @default.
- W2034201571 cites W2153355254 @default.
- W2034201571 cites W2153537688 @default.
- W2034201571 cites W2153650494 @default.
- W2034201571 cites W2155319224 @default.
- W2034201571 cites W2157838834 @default.
- W2034201571 cites W2163241233 @default.
- W2034201571 cites W2166139447 @default.