FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034205706> ?p ?o ?g. }

• W2034205706 endingPage "1664" @default.
• W2034205706 startingPage "1653" @default.
• W2034205706 abstract "Sequence determination of a region downstream from the vanXYC gene in Enterococcus gallinarum BM4174 revealed an open reading frame, designated vanT, that encodes a 698-amino-acid polypeptide with an amino-terminal domain containing 10 predicted transmembrane segments. The protein contained a highly conserved pyridoxal phosphate attachment site in the C-terminal domain, typical of alanine racemases. The protein was overexpressed in Escherichia coli, and serine racemase activity was detected in the membrane but not in the cytoplasmic fraction after centrifugation of sonicated cells, whereas alanine racemase activity was located almost exclusively in the cytoplasm. When the protein was overexpressed as a polypeptide lacking the predicted transmembrane domain, serine racemase activity was detected in the cytoplasm. The serine racemase activity was partially (64%) inhibited by d-cycloserine, whereas host alanine racemase activity was almost totally inhibited (97%). Serine racemase activity was also detected in membrane preparations of constitutively vancomycin-resistant E. gallinarum BM4174 but not in BM4175, in which insertional inactivation of the vanC-1d-Ala:d-Ser ligase gene probably had a polar effect on expression of the vanXYC and vanT genes. Comparative modelling of the deduced C-terminal domain was based on the alignment of VanT with the Alr alanine racemase from Bacillus stearothermophilus. The model revealed that almost all critical amino acids in the active site of Alr were conserved in VanT, indicating that the C-terminal domain of VanT is likely to adopt a three-dimensional structure similar to that of Alr and that the protein could exist as a dimer. These results indicate that the source of d-serine for peptidoglycan synthesis in vancomycin-resistant enterococci expressing the VanC phenotype involves racemization of l- to d-serine by a membrane-bound serine racemase." @default.
• W2034205706 created "2016-06-24" @default.
• W2034205706 creator A5001029093 @default.
• W2034205706 creator A5001281124 @default.
• W2034205706 creator A5027587575 @default.
• W2034205706 creator A5047471745 @default.
• W2034205706 creator A5051337635 @default.
• W2034205706 creator A5058899747 @default.
• W2034205706 date "1999-04-01" @default.
• W2034205706 modified "2023-10-18" @default.
• W2034205706 title "Characterization and modelling of VanT: a novel, membrane-bound, serine racemase from vancomycin-resistant Enterococcus gallinarum BM4174" @default.
• W2034205706 cites W1489250363 @default.
• W2034205706 cites W1520607277 @default.
• W2034205706 cites W1525582685 @default.
• W2034205706 cites W1525759724 @default.
• W2034205706 cites W1546685682 @default.
• W2034205706 cites W1607750614 @default.
• W2034205706 cites W1628177336 @default.
• W2034205706 cites W1777867218 @default.
• W2034205706 cites W1878478437 @default.
• W2034205706 cites W1879326539 @default.
• W2034205706 cites W1964832282 @default.
• W2034205706 cites W1966220928 @default.
• W2034205706 cites W1975304761 @default.
• W2034205706 cites W1986002586 @default.
• W2034205706 cites W1986191025 @default.
• W2034205706 cites W1986275834 @default.
• W2034205706 cites W1988381633 @default.
• W2034205706 cites W1990258186 @default.
• W2034205706 cites W1998195827 @default.
• W2034205706 cites W2001225885 @default.
• W2034205706 cites W2019929242 @default.
• W2034205706 cites W2020612301 @default.
• W2034205706 cites W2026368319 @default.
• W2034205706 cites W2026407337 @default.
• W2034205706 cites W2028231353 @default.
• W2034205706 cites W2036207120 @default.
• W2034205706 cites W2043014652 @default.
• W2034205706 cites W2044952215 @default.
• W2034205706 cites W2046505487 @default.
• W2034205706 cites W2052342483 @default.
• W2034205706 cites W2052811415 @default.
• W2034205706 cites W2063450941 @default.
• W2034205706 cites W2065283382 @default.
• W2034205706 cites W2071486470 @default.
• W2034205706 cites W2074092417 @default.
• W2034205706 cites W2074968287 @default.
• W2034205706 cites W2076694118 @default.
• W2034205706 cites W2080528351 @default.
• W2034205706 cites W2112702489 @default.
• W2034205706 cites W2114739684 @default.
• W2034205706 cites W2115727140 @default.
• W2034205706 cites W2128635872 @default.
• W2034205706 cites W2133392388 @default.
• W2034205706 cites W2138270253 @default.
• W2034205706 cites W2153668434 @default.
• W2034205706 cites W2159686532 @default.
• W2034205706 cites W3022750147 @default.
• W2034205706 cites W4236236547 @default.
• W2034205706 cites W4239880454 @default.
• W2034205706 cites W4242097652 @default.
• W2034205706 cites W4255084212 @default.
• W2034205706 doi "https://doi.org/10.1046/j.1365-2958.1999.01294.x" @default.
• W2034205706 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10209740" @default.
• W2034205706 hasPublicationYear "1999" @default.
• W2034205706 type Work @default.
• W2034205706 sameAs 2034205706 @default.
• W2034205706 citedByCount "68" @default.
• W2034205706 countsByYear W20342057062012 @default.
• W2034205706 countsByYear W20342057062014 @default.
• W2034205706 countsByYear W20342057062015 @default.
• W2034205706 countsByYear W20342057062016 @default.
• W2034205706 countsByYear W20342057062017 @default.
• W2034205706 countsByYear W20342057062018 @default.
• W2034205706 countsByYear W20342057062020 @default.
• W2034205706 countsByYear W20342057062021 @default.
• W2034205706 countsByYear W20342057062022 @default.
• W2034205706 countsByYear W20342057062023 @default.
• W2034205706 crossrefType "journal-article" @default.
• W2034205706 hasAuthorship W2034205706A5001029093 @default.
• W2034205706 hasAuthorship W2034205706A5001281124 @default.
• W2034205706 hasAuthorship W2034205706A5027587575 @default.
• W2034205706 hasAuthorship W2034205706A5047471745 @default.
• W2034205706 hasAuthorship W2034205706A5051337635 @default.
• W2034205706 hasAuthorship W2034205706A5058899747 @default.
• W2034205706 hasBestOaLocation W20342057061 @default.
• W2034205706 hasConcept C104317684 @default.
• W2034205706 hasConcept C118892022 @default.
• W2034205706 hasConcept C11960822 @default.
• W2034205706 hasConcept C153911025 @default.
• W2034205706 hasConcept C167625842 @default.
• W2034205706 hasConcept C2776414213 @default.
• W2034205706 hasConcept C2779856020 @default.
• W2034205706 hasConcept C515207424 @default.
• W2034205706 hasConcept C55493867 @default.
• W2034205706 hasConcept C86803240 @default.
• W2034205706 hasConceptScore W2034205706C104317684 @default.
• W2034205706 hasConceptScore W2034205706C118892022 @default.

• next