Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034206871> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W2034206871 endingPage "7795" @default.
- W2034206871 startingPage "7792" @default.
- W2034206871 abstract "Recently, we showed that completion of the polypeptide chains on the polysomes isolated from germinating rice seed scutellum in a cell-free translation system can direct the synthesis of (i) unprocessed polypeptide containing the signal sequence (precursor 1), (ii) signal sequence-cleaved but nonglycosylated polypeptide (precursor 2), and (iii) the fully processed and glycosylated form of alpha-amylase molecules. The two precursors as well as the mature form of alpha-amylase can thus be produced in almost the same condition. Here, the binding affinity of the enzyme molecule to the substrate analogue beta-cyclodextrin was used as a probe to compare the conformations of the three distinctly different polypeptide chains produced on the polysomes. It was found that the mature secretory form and the nonglycosylated precursor form (precursor 2) specifically bind to beta-cyclodextrin immobilized on an epoxy-activated Sepharose 6B column but the form that has an attached signal sequence (precursor 1) does not. The results provide evidence that the NH2-terminal signal sequence prevents acquisition of beta-cyclodextrin-binding activity, indicating that, in rice seed alpha-amylase, the signal sequence impairs conversion of the unprocessed polypeptide to the enzymically active configuration." @default.
- W2034206871 created "2016-06-24" @default.
- W2034206871 creator A5057085156 @default.
- W2034206871 creator A5088658720 @default.
- W2034206871 date "1982-12-01" @default.
- W2034206871 modified "2023-10-16" @default.
- W2034206871 title "alpha-Amylase biosynthesis: signal sequence prevents normal conversion of the unprocessed precursor molecule to the biologically active form." @default.
- W2034206871 cites W1527180528 @default.
- W2034206871 cites W1568061347 @default.
- W2034206871 cites W1987455517 @default.
- W2034206871 cites W1995449929 @default.
- W2034206871 cites W2051286266 @default.
- W2034206871 cites W2080494810 @default.
- W2034206871 cites W2095046337 @default.
- W2034206871 cites W2142001732 @default.
- W2034206871 cites W2146923243 @default.
- W2034206871 cites W2173897585 @default.
- W2034206871 doi "https://doi.org/10.1073/pnas.79.24.7792" @default.
- W2034206871 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/347434" @default.
- W2034206871 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6185952" @default.
- W2034206871 hasPublicationYear "1982" @default.
- W2034206871 type Work @default.
- W2034206871 sameAs 2034206871 @default.
- W2034206871 citedByCount "15" @default.
- W2034206871 crossrefType "journal-article" @default.
- W2034206871 hasAuthorship W2034206871A5057085156 @default.
- W2034206871 hasAuthorship W2034206871A5088658720 @default.
- W2034206871 hasBestOaLocation W20342068712 @default.
- W2034206871 hasConcept C104317684 @default.
- W2034206871 hasConcept C105580179 @default.
- W2034206871 hasConcept C10858879 @default.
- W2034206871 hasConcept C149364088 @default.
- W2034206871 hasConcept C167625842 @default.
- W2034206871 hasConcept C181199279 @default.
- W2034206871 hasConcept C185592680 @default.
- W2034206871 hasConcept C2778112365 @default.
- W2034206871 hasConcept C553450214 @default.
- W2034206871 hasConcept C55493867 @default.
- W2034206871 hasConcept C65666806 @default.
- W2034206871 hasConcept C67705224 @default.
- W2034206871 hasConcept C71240020 @default.
- W2034206871 hasConcept C86803240 @default.
- W2034206871 hasConcept C88478588 @default.
- W2034206871 hasConceptScore W2034206871C104317684 @default.
- W2034206871 hasConceptScore W2034206871C105580179 @default.
- W2034206871 hasConceptScore W2034206871C10858879 @default.
- W2034206871 hasConceptScore W2034206871C149364088 @default.
- W2034206871 hasConceptScore W2034206871C167625842 @default.
- W2034206871 hasConceptScore W2034206871C181199279 @default.
- W2034206871 hasConceptScore W2034206871C185592680 @default.
- W2034206871 hasConceptScore W2034206871C2778112365 @default.
- W2034206871 hasConceptScore W2034206871C553450214 @default.
- W2034206871 hasConceptScore W2034206871C55493867 @default.
- W2034206871 hasConceptScore W2034206871C65666806 @default.
- W2034206871 hasConceptScore W2034206871C67705224 @default.
- W2034206871 hasConceptScore W2034206871C71240020 @default.
- W2034206871 hasConceptScore W2034206871C86803240 @default.
- W2034206871 hasConceptScore W2034206871C88478588 @default.
- W2034206871 hasIssue "24" @default.
- W2034206871 hasLocation W20342068711 @default.
- W2034206871 hasLocation W20342068712 @default.
- W2034206871 hasLocation W20342068713 @default.
- W2034206871 hasOpenAccess W2034206871 @default.
- W2034206871 hasPrimaryLocation W20342068711 @default.
- W2034206871 hasRelatedWork W1570438659 @default.
- W2034206871 hasRelatedWork W1861462068 @default.
- W2034206871 hasRelatedWork W2050917233 @default.
- W2034206871 hasRelatedWork W2072604958 @default.
- W2034206871 hasRelatedWork W2387151289 @default.
- W2034206871 hasRelatedWork W2406535965 @default.
- W2034206871 hasRelatedWork W2572680590 @default.
- W2034206871 hasRelatedWork W2801483938 @default.
- W2034206871 hasRelatedWork W3039045748 @default.
- W2034206871 hasRelatedWork W3216818467 @default.
- W2034206871 hasVolume "79" @default.
- W2034206871 isParatext "false" @default.
- W2034206871 isRetracted "false" @default.
- W2034206871 magId "2034206871" @default.
- W2034206871 workType "article" @default.