FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034211019> ?p ?o ?g. }

• W2034211019 endingPage "132" @default.
• W2034211019 startingPage "127" @default.
• W2034211019 abstract "Previously published experiments had indicated unexpected expression of a control vector in which a β-galactosidase reporter was in the +1 reading frame relative to the translation start. This control vector contained the codon pair CCC CGA in the zero reading frame, raising the possibility that ribosomes rephased on this sequence, with peptidyl-tRNAPro pairing with CCC in the +1 frame. This putative rephasing might also be exacerbated by the rare CGA Arg codon in the second position due to increased vacancy of the ribosomal A-site. To test this hypothesis, a series of site-directed mutants was constructed, including mutations in both the first and second codons of this codon pair. The results show that interrupting the continuous run of C residues with synonymous codon changes essentially abolishes the frameshift. Further, changing the rare Arg codon to a common Arg codon also reduces the frequency of the frameshift. These results provide strong support for the hypothesis that CCC CGA in the zero frame is indeed a weak translational frameshift site in Escherichia coli, with a 1–2% efficiency. Because the vector sequence also contains another CCC triplet in the +1 reading frame starting within the next codon after the CGA, our data also support possible contribution to expression of a +7 nucleotide ribosome hop into the same +1 reading frame. We also confirm here a previous report that CCC UGA is a translational frameshift site, in these experiments, with about 5% efficiency." @default.
• W2034211019 created "2016-06-24" @default.
• W2034211019 creator A5019294726 @default.
• W2034211019 creator A5039868091 @default.
• W2034211019 creator A5053277853 @default.
• W2034211019 creator A5061706269 @default.
• W2034211019 date "2004-12-01" @default.
• W2034211019 modified "2023-10-10" @default.
• W2034211019 title "CCC CGA is a weak translational recoding site in Escherichia coli" @default.
• W2034211019 cites W1574779369 @default.
• W2034211019 cites W1577921631 @default.
• W2034211019 cites W1653521447 @default.
• W2034211019 cites W1905078083 @default.
• W2034211019 cites W1951212674 @default.
• W2034211019 cites W1970182817 @default.
• W2034211019 cites W1970969100 @default.
• W2034211019 cites W1974025425 @default.
• W2034211019 cites W1975077432 @default.
• W2034211019 cites W1975176460 @default.
• W2034211019 cites W1983634571 @default.
• W2034211019 cites W1989034594 @default.
• W2034211019 cites W2002031194 @default.
• W2034211019 cites W2006033507 @default.
• W2034211019 cites W2012456489 @default.
• W2034211019 cites W2014483367 @default.
• W2034211019 cites W2063504601 @default.
• W2034211019 cites W2066306545 @default.
• W2034211019 cites W2074400089 @default.
• W2034211019 cites W2079362122 @default.
• W2034211019 cites W2083890281 @default.
• W2034211019 cites W2119570097 @default.
• W2034211019 cites W2121423358 @default.
• W2034211019 cites W2129815091 @default.
• W2034211019 cites W2146240180 @default.
• W2034211019 cites W2160600702 @default.
• W2034211019 cites W2164930421 @default.
• W2034211019 cites W2174154048 @default.
• W2034211019 cites W2325916690 @default.
• W2034211019 cites W335857675 @default.
• W2034211019 cites W4239460348 @default.
• W2034211019 doi "https://doi.org/10.1016/j.gene.2004.08.012" @default.
• W2034211019 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15563838" @default.
• W2034211019 hasPublicationYear "2004" @default.
• W2034211019 type Work @default.
• W2034211019 sameAs 2034211019 @default.
• W2034211019 citedByCount "6" @default.
• W2034211019 countsByYear W20342110192015 @default.
• W2034211019 crossrefType "journal-article" @default.
• W2034211019 hasAuthorship W2034211019A5019294726 @default.
• W2034211019 hasAuthorship W2034211019A5039868091 @default.
• W2034211019 hasAuthorship W2034211019A5053277853 @default.
• W2034211019 hasAuthorship W2034211019A5061706269 @default.
• W2034211019 hasConcept C104317684 @default.
• W2034211019 hasConcept C105580179 @default.
• W2034211019 hasConcept C106774688 @default.
• W2034211019 hasConcept C114847915 @default.
• W2034211019 hasConcept C149364088 @default.
• W2034211019 hasConcept C166253671 @default.
• W2034211019 hasConcept C167625842 @default.
• W2034211019 hasConcept C176990463 @default.
• W2034211019 hasConcept C195491092 @default.
• W2034211019 hasConcept C20580545 @default.
• W2034211019 hasConcept C2775992057 @default.
• W2034211019 hasConcept C29906990 @default.
• W2034211019 hasConcept C47289529 @default.
• W2034211019 hasConcept C501734568 @default.
• W2034211019 hasConcept C54355233 @default.
• W2034211019 hasConcept C55334214 @default.
• W2034211019 hasConcept C67705224 @default.
• W2034211019 hasConcept C75563809 @default.
• W2034211019 hasConcept C86803240 @default.
• W2034211019 hasConcept C88478588 @default.
• W2034211019 hasConcept C95811502 @default.
• W2034211019 hasConceptScore W2034211019C104317684 @default.
• W2034211019 hasConceptScore W2034211019C105580179 @default.
• W2034211019 hasConceptScore W2034211019C106774688 @default.
• W2034211019 hasConceptScore W2034211019C114847915 @default.
• W2034211019 hasConceptScore W2034211019C149364088 @default.
• W2034211019 hasConceptScore W2034211019C166253671 @default.
• W2034211019 hasConceptScore W2034211019C167625842 @default.
• W2034211019 hasConceptScore W2034211019C176990463 @default.
• W2034211019 hasConceptScore W2034211019C195491092 @default.
• W2034211019 hasConceptScore W2034211019C20580545 @default.
• W2034211019 hasConceptScore W2034211019C2775992057 @default.
• W2034211019 hasConceptScore W2034211019C29906990 @default.
• W2034211019 hasConceptScore W2034211019C47289529 @default.
• W2034211019 hasConceptScore W2034211019C501734568 @default.
• W2034211019 hasConceptScore W2034211019C54355233 @default.
• W2034211019 hasConceptScore W2034211019C55334214 @default.
• W2034211019 hasConceptScore W2034211019C67705224 @default.
• W2034211019 hasConceptScore W2034211019C75563809 @default.
• W2034211019 hasConceptScore W2034211019C86803240 @default.
• W2034211019 hasConceptScore W2034211019C88478588 @default.
• W2034211019 hasConceptScore W2034211019C95811502 @default.
• W2034211019 hasIssue "1" @default.
• W2034211019 hasLocation W20342110191 @default.
• W2034211019 hasLocation W20342110192 @default.
• W2034211019 hasOpenAccess W2034211019 @default.

• next