FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034213780> ?p ?o ?g. }

• W2034213780 endingPage "212" @default.
• W2034213780 startingPage "205" @default.
• W2034213780 abstract "Falcipain-2 and -3 are cysteine proteases of erythrocytic Plasmodium falciparum parasites that appear to function principally as hemoglobinases. To better understand their biological roles, we analyzed the biosynthesis, localization, and processing of these enzymes in cultured parasites. Immunoprecipitation of metabolically labeled proteins indicated that falcipain-2 was synthesized through the trophozoite stage, falcipain-3 appeared in late trophozoites/early schizonts, and both proteases persisted for at least 6 h after synthesis. Falcipain-2 and -3 were localized to the food vacuole, the site of hemoglobin hydrolysis, by immunofluorescence and immunoelectron microscopy. Subcellular fractionation experiments indicated that both enzymes were synthesized as membrane bound proforms that were processed to soluble mature forms, but falcipain-2 was processed to the mature protease much more quickly than was falcipain-3. Cysteine protease inhibitors and brefeldin A, but not aspartic or serine protease inhibitors, blocked the processing of both enzymes, suggesting that falcipain-2 and -3 process by autohydrolysis after exiting the endoplasmic reticulum/Golgi network. However, although all tested cysteine protease inhibitors blocked hemoglobinase activity in the food vacuole, only lipophilic inhibitors (E-64d, Mu-Leu-Hph-VSPh, and ALLN), blocked intracellular processing of falcipain-2 and -3. More hydrophilic inhibitors (E-64 and leupeptin) did not block processing, suggesting that autocatalytic processing of falcipain-2 and -3 occurs in a specific cellular compartment before delivery to the food vacuole. Our results support overlapping but not fully redundant roles for falcipain-2 and -3, which are targeted to the food vacuole and activated sequentially to degrade hemoglobin in erythrocytic parasites." @default.
• W2034213780 created "2016-06-24" @default.
• W2034213780 creator A5077631479 @default.
• W2034213780 creator A5091387119 @default.
• W2034213780 date "2005-02-01" @default.
• W2034213780 modified "2023-09-24" @default.
• W2034213780 title "Biosynthesis, localization, and processing of falcipain cysteine proteases of Plasmodium falciparum" @default.
• W2034213780 cites W1589803235 @default.
• W2034213780 cites W1878275597 @default.
• W2034213780 cites W1964451754 @default.
• W2034213780 cites W1969807003 @default.
• W2034213780 cites W1976440024 @default.
• W2034213780 cites W1976757612 @default.
• W2034213780 cites W1977724107 @default.
• W2034213780 cites W1980846278 @default.
• W2034213780 cites W1997694813 @default.
• W2034213780 cites W2006569606 @default.
• W2034213780 cites W2015073280 @default.
• W2034213780 cites W2015578372 @default.
• W2034213780 cites W2020251171 @default.
• W2034213780 cites W2024771347 @default.
• W2034213780 cites W2029985793 @default.
• W2034213780 cites W2030855002 @default.
• W2034213780 cites W2038939970 @default.
• W2034213780 cites W2048371262 @default.
• W2034213780 cites W2051992027 @default.
• W2034213780 cites W2070828985 @default.
• W2034213780 cites W2074040429 @default.
• W2034213780 cites W2083762132 @default.
• W2034213780 cites W2085326030 @default.
• W2034213780 cites W2085887238 @default.
• W2034213780 cites W2112047656 @default.
• W2034213780 cites W2128378359 @default.
• W2034213780 cites W2130580441 @default.
• W2034213780 cites W2145226303 @default.
• W2034213780 cites W2157008176 @default.
• W2034213780 cites W2164365774 @default.
• W2034213780 cites W2166668583 @default.
• W2034213780 cites W2419648357 @default.
• W2034213780 cites W2913756671 @default.
• W2034213780 cites W4235339295 @default.
• W2034213780 doi "https://doi.org/10.1016/j.molbiopara.2004.11.009" @default.
• W2034213780 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15664655" @default.
• W2034213780 hasPublicationYear "2005" @default.
• W2034213780 type Work @default.
• W2034213780 sameAs 2034213780 @default.
• W2034213780 citedByCount "76" @default.
• W2034213780 countsByYear W20342137802012 @default.
• W2034213780 countsByYear W20342137802013 @default.
• W2034213780 countsByYear W20342137802014 @default.
• W2034213780 countsByYear W20342137802016 @default.
• W2034213780 countsByYear W20342137802018 @default.
• W2034213780 countsByYear W20342137802019 @default.
• W2034213780 countsByYear W20342137802020 @default.
• W2034213780 countsByYear W20342137802021 @default.
• W2034213780 countsByYear W20342137802022 @default.
• W2034213780 countsByYear W20342137802023 @default.
• W2034213780 crossrefType "journal-article" @default.
• W2034213780 hasAuthorship W2034213780A5077631479 @default.
• W2034213780 hasAuthorship W2034213780A5091387119 @default.
• W2034213780 hasConcept C102568950 @default.
• W2034213780 hasConcept C119062480 @default.
• W2034213780 hasConcept C158617107 @default.
• W2034213780 hasConcept C181199279 @default.
• W2034213780 hasConcept C182220744 @default.
• W2034213780 hasConcept C190062978 @default.
• W2034213780 hasConcept C203014093 @default.
• W2034213780 hasConcept C2775883766 @default.
• W2034213780 hasConcept C2776714187 @default.
• W2034213780 hasConcept C2777283782 @default.
• W2034213780 hasConcept C2778048844 @default.
• W2034213780 hasConcept C2778371730 @default.
• W2034213780 hasConcept C2781373781 @default.
• W2034213780 hasConcept C55493867 @default.
• W2034213780 hasConcept C86803240 @default.
• W2034213780 hasConcept C95444343 @default.
• W2034213780 hasConceptScore W2034213780C102568950 @default.
• W2034213780 hasConceptScore W2034213780C119062480 @default.
• W2034213780 hasConceptScore W2034213780C158617107 @default.
• W2034213780 hasConceptScore W2034213780C181199279 @default.
• W2034213780 hasConceptScore W2034213780C182220744 @default.
• W2034213780 hasConceptScore W2034213780C190062978 @default.
• W2034213780 hasConceptScore W2034213780C203014093 @default.
• W2034213780 hasConceptScore W2034213780C2775883766 @default.
• W2034213780 hasConceptScore W2034213780C2776714187 @default.
• W2034213780 hasConceptScore W2034213780C2777283782 @default.
• W2034213780 hasConceptScore W2034213780C2778048844 @default.
• W2034213780 hasConceptScore W2034213780C2778371730 @default.
• W2034213780 hasConceptScore W2034213780C2781373781 @default.
• W2034213780 hasConceptScore W2034213780C55493867 @default.
• W2034213780 hasConceptScore W2034213780C86803240 @default.
• W2034213780 hasConceptScore W2034213780C95444343 @default.
• W2034213780 hasIssue "2" @default.
• W2034213780 hasLocation W20342137801 @default.
• W2034213780 hasLocation W20342137802 @default.
• W2034213780 hasOpenAccess W2034213780 @default.
• W2034213780 hasPrimaryLocation W20342137801 @default.
• W2034213780 hasRelatedWork W1993690543 @default.

• next