Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034213889> ?p ?o ?g. }
Showing items 1 to 82 of
82
with 100 items per page.
- W2034213889 abstract "Several dyes were evaluated for their potential use as indices of ionic boundaries and pH discontinuities during polyacrylamide gel electrophoresis (PAGE). Dye selection was based largely upon color transition over the pH intervals present in the commonly used Tris-HCl-glycine buffer systems. In the absence of sodium dodecylsulfate (SDS) nitrazine yellow, cresol purple, cresol red, and phenol red had relative mobilities (relative to the chloride boundary; RC1) of 1.00 at 20% polyacrylamide concentration (% T) compared to bromophenol blue (BPB) for which RC1=0.77. The homologue of BPB, 3, 4, 5, 6-tetrabromophenolsulfonephthalein (TBPS), exhibited a useful color transition over the pH 6.6-8.2 range but was an equally poor indicator of the chloride boundary at 20% T in the absence of SDS (RC1=0.73). The RC1 of other dyes was increased in the presence of SDS, particularly the cationic dye neutral red which complexes with micellar but not monomeric SDS. In the presence of SDS, all dyes except neutral red exhibited RC1 of 1.00 at 15% T, whereas only neutral red and nitrazine yellow had RC1 values of 1.00 at lower gel concentrations. Partial characterization of SDS, in terms of RC1 and zone broadening, was performed in a system devoid of extraneous SDS in the gel or electrolytes." @default.
- W2034213889 created "2016-06-24" @default.
- W2034213889 creator A5075157734 @default.
- W2034213889 date "2001-01-01" @default.
- W2034213889 modified "2023-10-03" @default.
- W2034213889 title "Color transitional dyes as indicators of ionic boundaries in sodium dodecylsulfate polyacrylamide gel electrophoresis." @default.
- W2034213889 cites W1975085160 @default.
- W2034213889 cites W1976979378 @default.
- W2034213889 cites W2036537163 @default.
- W2034213889 cites W2047903470 @default.
- W2034213889 cites W2051122489 @default.
- W2034213889 cites W2054631692 @default.
- W2034213889 cites W2069185960 @default.
- W2034213889 cites W2100837269 @default.
- W2034213889 cites W2111301002 @default.
- W2034213889 cites W2132068224 @default.
- W2034213889 cites W2316346216 @default.
- W2034213889 cites W2327809144 @default.
- W2034213889 cites W2584933950 @default.
- W2034213889 doi "https://doi.org/10.2198/sbk.45.75" @default.
- W2034213889 hasPublicationYear "2001" @default.
- W2034213889 type Work @default.
- W2034213889 sameAs 2034213889 @default.
- W2034213889 citedByCount "1" @default.
- W2034213889 crossrefType "journal-article" @default.
- W2034213889 hasAuthorship W2034213889A5075157734 @default.
- W2034213889 hasBestOaLocation W20342138891 @default.
- W2034213889 hasConcept C101660505 @default.
- W2034213889 hasConcept C145148216 @default.
- W2034213889 hasConcept C178790620 @default.
- W2034213889 hasConcept C179104552 @default.
- W2034213889 hasConcept C181199279 @default.
- W2034213889 hasConcept C185592680 @default.
- W2034213889 hasConcept C188027245 @default.
- W2034213889 hasConcept C2182769 @default.
- W2034213889 hasConcept C2778450494 @default.
- W2034213889 hasConcept C2778695967 @default.
- W2034213889 hasConcept C2778862431 @default.
- W2034213889 hasConcept C2780418270 @default.
- W2034213889 hasConcept C43617362 @default.
- W2034213889 hasConcept C537181965 @default.
- W2034213889 hasConceptScore W2034213889C101660505 @default.
- W2034213889 hasConceptScore W2034213889C145148216 @default.
- W2034213889 hasConceptScore W2034213889C178790620 @default.
- W2034213889 hasConceptScore W2034213889C179104552 @default.
- W2034213889 hasConceptScore W2034213889C181199279 @default.
- W2034213889 hasConceptScore W2034213889C185592680 @default.
- W2034213889 hasConceptScore W2034213889C188027245 @default.
- W2034213889 hasConceptScore W2034213889C2182769 @default.
- W2034213889 hasConceptScore W2034213889C2778450494 @default.
- W2034213889 hasConceptScore W2034213889C2778695967 @default.
- W2034213889 hasConceptScore W2034213889C2778862431 @default.
- W2034213889 hasConceptScore W2034213889C2780418270 @default.
- W2034213889 hasConceptScore W2034213889C43617362 @default.
- W2034213889 hasConceptScore W2034213889C537181965 @default.
- W2034213889 hasLocation W20342138891 @default.
- W2034213889 hasOpenAccess W2034213889 @default.
- W2034213889 hasPrimaryLocation W20342138891 @default.
- W2034213889 hasRelatedWork W138725216 @default.
- W2034213889 hasRelatedWork W1848163424 @default.
- W2034213889 hasRelatedWork W1964510700 @default.
- W2034213889 hasRelatedWork W1967302442 @default.
- W2034213889 hasRelatedWork W1971030124 @default.
- W2034213889 hasRelatedWork W2007906942 @default.
- W2034213889 hasRelatedWork W2025332767 @default.
- W2034213889 hasRelatedWork W2026858819 @default.
- W2034213889 hasRelatedWork W2034344797 @default.
- W2034213889 hasRelatedWork W2044300273 @default.
- W2034213889 hasRelatedWork W2045280807 @default.
- W2034213889 hasRelatedWork W2055889114 @default.
- W2034213889 hasRelatedWork W2367726506 @default.
- W2034213889 hasRelatedWork W2373094048 @default.
- W2034213889 hasRelatedWork W2396365385 @default.
- W2034213889 hasRelatedWork W2437812077 @default.
- W2034213889 hasRelatedWork W3081575821 @default.
- W2034213889 hasRelatedWork W2189273426 @default.
- W2034213889 hasRelatedWork W2483934967 @default.
- W2034213889 hasRelatedWork W3141822746 @default.
- W2034213889 isParatext "false" @default.
- W2034213889 isRetracted "false" @default.
- W2034213889 magId "2034213889" @default.
- W2034213889 workType "article" @default.