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• W2034215779 startingPage "e6058" @default.
• W2034215779 abstract "Formulation of DNA/cationic lipid complexes (lipoplexes) designed for nucleic acid delivery mostly results in positively charged particles which are thought to enter cells by endocytosis. We recently developed a lipoplex formulation called Neutraplex that allows preparation of both cationic and anionic stable complexes with similar lipid content and ultrastructure.To assess whether the global net charge could influence cell uptake and activity of the transported oligonucleotides (on), we prepared lipoplexes with positive and negative charges and compared: (i) their physicochemical properties by zeta potential analysis and dynamic light scattering, (ii) their cell uptake by fluorescence microscopy and flow cytometry, and (iii) the biological activity of the transported ON using a splicing correction assay. We show that positively or negatively charged lipoplexes enter cells cells using both temperature-dependent and -independent uptake mechanisms. Specifically, positively charged lipoplexes predominantly use a temperature-dependent transport when cells are incubated OptiMEM medium. Anionic lipoplexes favour an energy-independent transport and show higher ON activity than cationic lipoplexes in presence of serum. However, lipoplexes with high positive global net charge and OptiMEM medium give the highest uptake and ON activity levels.These findings suggest that, in addition to endocytosis, lipoplexes may enter cell via a temperature-independent mechanism, which could be mediated by lipid mixing. Such characteristics might arise from the specific lipoplex ultrastructure and should be taken into consideration when developing lipoplexes designed for in vivo or ex vivo nucleic acid transfer." @default.
• W2034215779 created "2016-06-24" @default.
• W2034215779 creator A5009596243 @default.
• W2034215779 creator A5081714251 @default.
• W2034215779 creator A5088629839 @default.
• W2034215779 date "2009-06-26" @default.
• W2034215779 modified "2023-09-23" @default.
• W2034215779 title "Physico-Chemical Characteristics of Lipoplexes Influence Cell Uptake Mechanisms and Transfection Efficacy" @default.
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• W2034215779 doi "https://doi.org/10.1371/journal.pone.0006058" @default.
• W2034215779 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2699663" @default.
• W2034215779 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19557145" @default.
• W2034215779 hasPublicationYear "2009" @default.
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