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• W2034219151 abstract "In this study, the separation efficiency of a core–shell particle column was compared with particle-packed and monolithic silica columns, which showed that the core–shell particle column had a smaller theoretical plate height and that its separation efficiency was not affected significantly by the increase in flow rate. A fast HPLC method using a core–shell particle column was developed for the determination of amino acids. 4-Fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) was used as a fluorescence derivatization reagent for amino acids, followed by separation on a core–shell Kinetex C18 column. The analysis time for 21 NBD-amino acids was within 7 min, which was faster than that in our previous studies with conventional particle-packed columns or monolithic silica columns. The linearities of the calibration curves for all the amino acids were found to be good over a range of injection amounts from 40 fmol to 40 pmol. The accuracies for the amino acid determinations were 90.9–107%. The method was proved to have potential for the fast determination of amino acids in biological samples." @default.
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• W2034219151 date "2013-05-01" @default.
• W2034219151 modified "2023-10-16" @default.
• W2034219151 title "Amino acid analysis using core–shell particle column" @default.
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• W2034219151 doi "https://doi.org/10.1016/j.jchromb.2012.09.005" @default.
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