FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034227308> ?p ?o ?g. }

• W2034227308 endingPage "11455" @default.
• W2034227308 startingPage "11451" @default.
• W2034227308 abstract "Amino acid sequencing by recombinant DNA technology, although dramatically useful, is subject to base reading errors, is indirect, and is insensitive to posttranslational processing. Mass spectrometry techniques can provide molecular weight data from even relatively large proteins for such cDNA sequences and can serve as a check of an enzyme's purity and sequence integrity. Multiply-charged ions from electrospray ionization can be dissociated to yield structural information by tandem mass spectrometry, providing a second method for gaining additional confidence in primary sequence confirmation. Here, accurate (+/- 1 Da) molecular weight and molecular ion dissociation information for human muscle and brain creatine kinases has been obtained by electrospray ionization coupled with Fourier-transform mass spectrometry to help distinguish which of several published amino acid sequences for both enzymes are correct. The results herein are consistent with one published sequence for each isozyme, and the heterogeneity indicated by isoelectric focusing due to 1-Da deamidation changes. This approach appears generally useful for detailed sequence verification of recombinant proteins." @default.
• W2034227308 created "2016-06-24" @default.
• W2034227308 creator A5002052649 @default.
• W2034227308 creator A5013789390 @default.
• W2034227308 creator A5020002876 @default.
• W2034227308 creator A5028893802 @default.
• W2034227308 creator A5064393869 @default.
• W2034227308 creator A5076358242 @default.
• W2034227308 date "1995-12-05" @default.
• W2034227308 modified "2023-10-13" @default.
• W2034227308 title "Sequence verification of human creatine kinase (43 kDa) isozymes by high-resolution tandem mass spectrometry." @default.
• W2034227308 cites W1528793678 @default.
• W2034227308 cites W1555311619 @default.
• W2034227308 cites W1569136570 @default.
• W2034227308 cites W1576964321 @default.
• W2034227308 cites W1988105291 @default.
• W2034227308 cites W2001321345 @default.
• W2034227308 cites W2001962326 @default.
• W2034227308 cites W2008532321 @default.
• W2034227308 cites W2015153974 @default.
• W2034227308 cites W2018792069 @default.
• W2034227308 cites W2021710187 @default.
• W2034227308 cites W2025736749 @default.
• W2034227308 cites W2037863214 @default.
• W2034227308 cites W2043201277 @default.
• W2034227308 cites W2043690278 @default.
• W2034227308 cites W2047534746 @default.
• W2034227308 cites W2058446582 @default.
• W2034227308 cites W2069039856 @default.
• W2034227308 cites W2069611679 @default.
• W2034227308 cites W2083991421 @default.
• W2034227308 cites W2085145164 @default.
• W2034227308 cites W2086348243 @default.
• W2034227308 cites W2139771382 @default.
• W2034227308 doi "https://doi.org/10.1073/pnas.92.25.11451" @default.
• W2034227308 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/40419" @default.
• W2034227308 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8876261" @default.
• W2034227308 hasPublicationYear "1995" @default.
• W2034227308 type Work @default.
• W2034227308 sameAs 2034227308 @default.
• W2034227308 citedByCount "28" @default.
• W2034227308 countsByYear W20342273082013 @default.
• W2034227308 countsByYear W20342273082015 @default.
• W2034227308 countsByYear W20342273082016 @default.
• W2034227308 countsByYear W20342273082019 @default.
• W2034227308 countsByYear W20342273082020 @default.
• W2034227308 countsByYear W20342273082021 @default.
• W2034227308 countsByYear W20342273082022 @default.
• W2034227308 crossrefType "journal-article" @default.
• W2034227308 hasAuthorship W2034227308A5002052649 @default.
• W2034227308 hasAuthorship W2034227308A5013789390 @default.
• W2034227308 hasAuthorship W2034227308A5020002876 @default.
• W2034227308 hasAuthorship W2034227308A5028893802 @default.
• W2034227308 hasAuthorship W2034227308A5064393869 @default.
• W2034227308 hasAuthorship W2034227308A5076358242 @default.
• W2034227308 hasBestOaLocation W20342273081 @default.
• W2034227308 hasConcept C104317684 @default.
• W2034227308 hasConcept C162356407 @default.
• W2034227308 hasConcept C167625842 @default.
• W2034227308 hasConcept C181199279 @default.
• W2034227308 hasConcept C185592680 @default.
• W2034227308 hasConcept C202157981 @default.
• W2034227308 hasConcept C2780784506 @default.
• W2034227308 hasConcept C31827203 @default.
• W2034227308 hasConcept C40684141 @default.
• W2034227308 hasConcept C43617362 @default.
• W2034227308 hasConcept C46111723 @default.
• W2034227308 hasConcept C55493867 @default.
• W2034227308 hasConcept C80311884 @default.
• W2034227308 hasConcept C86153913 @default.
• W2034227308 hasConceptScore W2034227308C104317684 @default.
• W2034227308 hasConceptScore W2034227308C162356407 @default.
• W2034227308 hasConceptScore W2034227308C167625842 @default.
• W2034227308 hasConceptScore W2034227308C181199279 @default.
• W2034227308 hasConceptScore W2034227308C185592680 @default.
• W2034227308 hasConceptScore W2034227308C202157981 @default.
• W2034227308 hasConceptScore W2034227308C2780784506 @default.
• W2034227308 hasConceptScore W2034227308C31827203 @default.
• W2034227308 hasConceptScore W2034227308C40684141 @default.
• W2034227308 hasConceptScore W2034227308C43617362 @default.
• W2034227308 hasConceptScore W2034227308C46111723 @default.
• W2034227308 hasConceptScore W2034227308C55493867 @default.
• W2034227308 hasConceptScore W2034227308C80311884 @default.
• W2034227308 hasConceptScore W2034227308C86153913 @default.
• W2034227308 hasIssue "25" @default.
• W2034227308 hasLocation W20342273081 @default.
• W2034227308 hasLocation W20342273082 @default.
• W2034227308 hasLocation W20342273083 @default.
• W2034227308 hasLocation W20342273084 @default.
• W2034227308 hasLocation W20342273085 @default.
• W2034227308 hasOpenAccess W2034227308 @default.
• W2034227308 hasPrimaryLocation W20342273081 @default.
• W2034227308 hasRelatedWork W1985459812 @default.
• W2034227308 hasRelatedWork W1990735017 @default.
• W2034227308 hasRelatedWork W2008863376 @default.
• W2034227308 hasRelatedWork W2033561045 @default.
• W2034227308 hasRelatedWork W2034227308 @default.
• W2034227308 hasRelatedWork W2040286337 @default.

• next