Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034228449> ?p ?o ?g. }
Showing items 1 to 61 of
61
with 100 items per page.
- W2034228449 abstract "A novel column for spiral countercurrent chromatography (CCC) has been designed to interrupt the laminar flow of the mobile phase through the tubing and continuously mix the two phases throughout the column [1]. Flat-twisted tubing system was originally introduced for purification of large molecules (proteins and peptides). Its application in the separation of small molecules is presented for the first time. Here pH-zone-refining CCC is successfully applied to the preparative separation of Picralima alkaloids using this new tubing filled in the spiral tubing support rotor (STS, CC Biotech, Rockville MD). The seeds of Picralima nitida (Apocynaceae), popularly known in African ethnomedicine for its antimalarial activity, were extracted successively with solvents of increasing polarity to obtain hexane, dichloromethane (DCM) and methanol (MeOH) extracts. The experiment was performed with a two-phase solvent system composed of t-methyl-butyl ether-n-butanol-acetonitrile-water at an optimized volume ratio of 2:2:1:5 (v/v) where triethylamine (5mM) was added to the upper organic stationary phase as a retainer and hydrochloric acid (5 mM) to the aqueous mobile phase as an eluter. Two separation runs of 4.0g each of the DCM and MeOH extracts yielded six major indole alkaloids including akuammine, akuammicine, alstonine, picraline, Melinonine A and akuammidine with purities ranging from 90% (Melinonine A) to 98% (akuammine) as determined by HPLC. Overall, the results revealed that the new Flat-twisted tubing system yielded high partition efficiency with a satisfactory level of stationary phase retention in a short elution time." @default.
- W2034228449 created "2016-06-24" @default.
- W2034228449 creator A5001015284 @default.
- W2034228449 creator A5008037071 @default.
- W2034228449 creator A5021451732 @default.
- W2034228449 creator A5029121616 @default.
- W2034228449 creator A5081691633 @default.
- W2034228449 creator A5087354083 @default.
- W2034228449 date "2011-03-01" @default.
- W2034228449 modified "2023-09-27" @default.
- W2034228449 title "Preparative Separation of Picralima Alkaloids using a New Flat-Twisted Tubing in pH-Zone-Refining Countercurrent Chromatography Mode" @default.
- W2034228449 doi "https://doi.org/10.1055/s-0031-1273630" @default.
- W2034228449 hasPublicationYear "2011" @default.
- W2034228449 type Work @default.
- W2034228449 sameAs 2034228449 @default.
- W2034228449 citedByCount "1" @default.
- W2034228449 countsByYear W20342284492012 @default.
- W2034228449 crossrefType "journal-article" @default.
- W2034228449 hasAuthorship W2034228449A5001015284 @default.
- W2034228449 hasAuthorship W2034228449A5008037071 @default.
- W2034228449 hasAuthorship W2034228449A5021451732 @default.
- W2034228449 hasAuthorship W2034228449A5029121616 @default.
- W2034228449 hasAuthorship W2034228449A5081691633 @default.
- W2034228449 hasAuthorship W2034228449A5087354083 @default.
- W2034228449 hasConcept C12898812 @default.
- W2034228449 hasConcept C178790620 @default.
- W2034228449 hasConcept C179998833 @default.
- W2034228449 hasConcept C185592680 @default.
- W2034228449 hasConcept C2780471494 @default.
- W2034228449 hasConcept C2781283055 @default.
- W2034228449 hasConcept C43617362 @default.
- W2034228449 hasConcept C4725764 @default.
- W2034228449 hasConcept C63258622 @default.
- W2034228449 hasConceptScore W2034228449C12898812 @default.
- W2034228449 hasConceptScore W2034228449C178790620 @default.
- W2034228449 hasConceptScore W2034228449C179998833 @default.
- W2034228449 hasConceptScore W2034228449C185592680 @default.
- W2034228449 hasConceptScore W2034228449C2780471494 @default.
- W2034228449 hasConceptScore W2034228449C2781283055 @default.
- W2034228449 hasConceptScore W2034228449C43617362 @default.
- W2034228449 hasConceptScore W2034228449C4725764 @default.
- W2034228449 hasConceptScore W2034228449C63258622 @default.
- W2034228449 hasIssue "05" @default.
- W2034228449 hasLocation W20342284491 @default.
- W2034228449 hasOpenAccess W2034228449 @default.
- W2034228449 hasPrimaryLocation W20342284491 @default.
- W2034228449 hasRelatedWork W1588095382 @default.
- W2034228449 hasRelatedWork W2016713066 @default.
- W2034228449 hasRelatedWork W2018671480 @default.
- W2034228449 hasRelatedWork W2018816110 @default.
- W2034228449 hasRelatedWork W2022333823 @default.
- W2034228449 hasRelatedWork W2063013405 @default.
- W2034228449 hasRelatedWork W2066554088 @default.
- W2034228449 hasRelatedWork W2125985250 @default.
- W2034228449 hasRelatedWork W2376531500 @default.
- W2034228449 hasRelatedWork W2911853957 @default.
- W2034228449 hasVolume "77" @default.
- W2034228449 isParatext "false" @default.
- W2034228449 isRetracted "false" @default.
- W2034228449 magId "2034228449" @default.
- W2034228449 workType "article" @default.