FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034234265> ?p ?o ?g. }

• W2034234265 endingPage "3687" @default.
• W2034234265 startingPage "3687" @default.
• W2034234265 abstract "purpose. To compare PDGF- and insulin/IGF-1–induced class IA PI 3-kinase/Akt survival signaling in normal retinas and retinal ganglion cells (RGCs). methods. Normal rat retinas and RGC-5 cells were used for (1) immunohistochemical and immunoblot studies to detect PDGF receptor (PDGFR) subtypes and (2) immunoprecipitation, immunoblot, and in vitro lipid kinase assays to determine basal and PDGF-induced class IA PI 3-kinase/Akt survival signaling, in comparison with insulin or IGF-1 responses. Furthermore, RGC-5 cells were exposed to broad-spectrum (LY294002) or p110 isoform-selective (PI-103) PI 3-kinase inhibitors (versus Akt inhibitor) to assess the consequent effects on Akt phosphorylation, caspase-3/PARP cleavage, apoptotic phenotype, and cell viability, as a function of serum trophic factors. results. PDGFR-α and -β immunoreactivity was observed in rat retinal Müller cells and in the RGC layer and blood vessels, respectively. In addition, PDGFR-α and -β protein expression was observed in RGC-5 cells. Both retinas and RGC-5 cells exhibited a similar pattern of subunit-specific basal class IA PI 3-kinase activity, which was stimulated in a temporal and signal-specific manner by PDGF and insulin/IGF-1. Furthermore, RGC-5 cells showed PDGFR-α/β tyrosine phosphorylation that induced the p85α regulatory subunit to activate p110α/β-associated class IA PI 3-kinase, which in turn enhanced Akt phosphorylation. Exposure of serum-deprived RGC-5 cells to PI 3-kinase or Akt inhibitors increased susceptibility to apoptotic phenotype as revealed by caspase-3 and PARP cleavage. conclusions. The present findings provide direct evidence of two distinct modes of retinal class IA PI 3-kinase activation that occurs in response to PDGF receptor and insulin/IGF-1 receptor stimulation. PDGF-induced PI 3-kinase/PIP3/Akt axis may provide new therapeutic approaches to ameliorate cell death in diabetic retinopathy and other retinal neurodegenerations." @default.
• W2034234265 created "2016-06-24" @default.
• W2034234265 creator A5035543524 @default.
• W2034234265 creator A5051046891 @default.
• W2034234265 creator A5056264759 @default.
• W2034234265 creator A5061569416 @default.
• W2034234265 creator A5084965091 @default.
• W2034234265 date "2008-08-01" @default.
• W2034234265 modified "2023-09-26" @default.
• W2034234265 title "PDGF- and Insulin/IGF-1–Specific Distinct Modes of Class I_API 3-Kinase Activation in Normal Rat Retinas and RGC-5 Retinal Ganglion Cells" @default.
• W2034234265 cites W148296933 @default.
• W2034234265 cites W1489750542 @default.
• W2034234265 cites W1563169973 @default.
• W2034234265 cites W1580278923 @default.
• W2034234265 cites W158152021 @default.
• W2034234265 cites W1604048158 @default.
• W2034234265 cites W1651927573 @default.
• W2034234265 cites W1836215938 @default.
• W2034234265 cites W1896108812 @default.
• W2034234265 cites W1972239887 @default.
• W2034234265 cites W1973418567 @default.
• W2034234265 cites W1974159373 @default.
• W2034234265 cites W1978271705 @default.
• W2034234265 cites W1982155207 @default.
• W2034234265 cites W1982711630 @default.
• W2034234265 cites W1986824675 @default.
• W2034234265 cites W1988224683 @default.
• W2034234265 cites W1995641301 @default.
• W2034234265 cites W1999012962 @default.
• W2034234265 cites W2002940556 @default.
• W2034234265 cites W2004831648 @default.
• W2034234265 cites W2005895705 @default.
• W2034234265 cites W2009717367 @default.
• W2034234265 cites W2015338496 @default.
• W2034234265 cites W2016939970 @default.
• W2034234265 cites W2025388522 @default.
• W2034234265 cites W2027690069 @default.
• W2034234265 cites W2028648973 @default.
• W2034234265 cites W2033246537 @default.
• W2034234265 cites W2033999408 @default.
• W2034234265 cites W2035263256 @default.
• W2034234265 cites W2037653840 @default.
• W2034234265 cites W2038399499 @default.
• W2034234265 cites W2038403792 @default.
• W2034234265 cites W2045726368 @default.
• W2034234265 cites W2045884328 @default.
• W2034234265 cites W2051675190 @default.
• W2034234265 cites W2059072734 @default.
• W2034234265 cites W2066963022 @default.
• W2034234265 cites W2067327808 @default.
• W2034234265 cites W2070468104 @default.
• W2034234265 cites W2071695311 @default.
• W2034234265 cites W2074182701 @default.
• W2034234265 cites W2079792463 @default.
• W2034234265 cites W2087473057 @default.
• W2034234265 cites W2089109099 @default.
• W2034234265 cites W2090458886 @default.
• W2034234265 cites W2091299313 @default.
• W2034234265 cites W2092901424 @default.
• W2034234265 cites W2095349122 @default.
• W2034234265 cites W2095960119 @default.
• W2034234265 cites W2100694105 @default.
• W2034234265 cites W2102028715 @default.
• W2034234265 cites W2103122477 @default.
• W2034234265 cites W2110121929 @default.
• W2034234265 cites W2114834794 @default.
• W2034234265 cites W2115484306 @default.
• W2034234265 cites W2118099034 @default.
• W2034234265 cites W2122236318 @default.
• W2034234265 cites W2124051484 @default.
• W2034234265 cites W2124593509 @default.
• W2034234265 cites W2125294887 @default.
• W2034234265 cites W2129221351 @default.
• W2034234265 cites W2135244179 @default.
• W2034234265 cites W2139270233 @default.
• W2034234265 cites W2153008086 @default.
• W2034234265 cites W2164139183 @default.
• W2034234265 cites W2169813593 @default.
• W2034234265 cites W2322846418 @default.
• W2034234265 cites W2344973376 @default.
• W2034234265 cites W2467054703 @default.
• W2034234265 cites W33092065 @default.
• W2034234265 cites W46054612 @default.
• W2034234265 cites W53230035 @default.
• W2034234265 doi "https://doi.org/10.1167/iovs.07-1455" @default.
• W2034234265 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18421086" @default.
• W2034234265 hasPublicationYear "2008" @default.
• W2034234265 type Work @default.
• W2034234265 sameAs 2034234265 @default.
• W2034234265 citedByCount "24" @default.
• W2034234265 countsByYear W20342342652013 @default.
• W2034234265 countsByYear W20342342652014 @default.
• W2034234265 countsByYear W20342342652016 @default.
• W2034234265 countsByYear W20342342652017 @default.
• W2034234265 countsByYear W20342342652018 @default.
• W2034234265 countsByYear W20342342652021 @default.
• W2034234265 countsByYear W20342342652022 @default.
• W2034234265 crossrefType "journal-article" @default.

• next