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- W2034239517 abstract "1 Chick embryo fibroblasts, that cease to grow after a finite number of passages in culture, have been cultured in the presence of radioactive d-glucosamine. Incorporation of radioactivity into cellular macromolecules is very similar for cells regardless of their position in the finite life span. 2 The labelled substances removed by trypsin from the surface of non-confluent cells growing in different parts of the life span were analysed. Smooth membrane fractions isolated from trypsinised cells were also compared. 3 The labelled substances were very similar, in particular the extent of sialylation of the total glycoprotein fraction of early phase or late phase cells. Minor differences were found, however. 4 It was found that mucopolysaccharides constitute a larger proportion of the labelled material from the surface of late phase cells than from the surface of early phase cells. Conversely early phase cells were relatively rich in several glycopeptides compared to late phase cells." @default.
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- W2034239517 date "1974-05-01" @default.
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- W2034239517 title "Glycoproteins of Chick Embryo Fibroblasts in Cultures with a Finite Life Span" @default.
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- W2034239517 doi "https://doi.org/10.1111/j.1432-1033.1974.tb03465.x" @default.
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