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- W2034240458 abstract "We report here the use of an intein-mediated protein ligation strategy for the synthesis of glycoproteins and proteins containing various molecular probes. Several simple cysteine derivatives are synthesized and used as nucleophiles for specific incorporation onto the C-terminus of proteins expressed as N-terminal intein fusions. Examples of C-terminal carbohydrate labeling, fluorescence labeling, incorporation of a metal chelator, and incorporation of a nucleotide are presented." @default.
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- W2034240458 date "2000-05-27" @default.
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- W2034240458 title "Intein-Mediated Synthesis of Proteins Containing Carbohydrates and Other Molecular Probes" @default.
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- W2034240458 doi "https://doi.org/10.1021/ja0000192" @default.
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