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• W2034241007 abstract "<h2>Abstract</h2> The generation of leukotrienes and histamine release by the mouse mastocytoma cell line MMC-16 was investigated. These cells produced leukotriene C₄ (LTC₄) and released histamine upon calcium ionophore A23187 and antigen stimulation. The ionophore also stimulated the biosynthesis of leukotriene B₄ (LTB₄) by MMC-16. Generation of LTC₄ was confirmed by its characteristic UV absorption spectrum, fast atom bombardment-MS, equivalent HPLC retention time with an authentic standard and radioimmunoassay. Leukotriene B₄ was characterized by its distinctive UV spectrum and HPLC retention time compared with synthetic material. IgE-mediated LTC₄ generation was also observed in a dose dependent fashion with MMC-16 cells passively sensitized with monoclonal IgE specific for ovalbumin. LTC₄ biosynthesis was effectively inhibited by the lipoxygenase inhibitor NDGA." @default.
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• W2034241007 date "1988-03-01" @default.
• W2034241007 modified "2023-10-18" @default.
• W2034241007 title "Generation of leukotrienes by antigen and calcium lonophore A23187 stimuli from a mouse mastocytoma cell line, MMC-16" @default.
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• W2034241007 doi "https://doi.org/10.1016/0952-3278(88)90111-1" @default.
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