FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034246190> ?p ?o ?g. }

• W2034246190 endingPage "92" @default.
• W2034246190 startingPage "84" @default.
• W2034246190 abstract "Using pBR322- and pUC-derived plasmid vectors, a homologous (Escherichia coli native esterase) and three heterologous proteins (human interleukin-2, human interleukin-6, and Zymomonas levansucrase) were synthesized in E. coli IC2015(recA::lacZ) and GY4786 (sfiA::lacZ) strains. Via time-course measurement of β-galactosidase activity in each recombinant culture, the SOS induction was estimated in detail and the results were systematically compared. In recombinant E. coli, the SOS response did not happen either with the recombinant insert-negative plasmid backbone alone or the expression vectors containing the homologous gene. Irrespective of gene expression level and toxic activity of synthesized foreign proteins, the SOS response was induced only when the heterologous genes were expressed using a particular plasmid vector, indicating strong dependence on the recombinant gene clone and the selection of a plasmid vector system. It is suggested that in recombinant E. coli the SOS response (i.e., activation of recA expression and initial sfiA expression) may be related neither to metabolic burden nor toxic cellular event(s) by synthesized heterologous protein, but may be provoked by foreign gene-specific interaction between a foreign gene and a plasmid vector. Unlike in E. coli XL1-blue(recA−) strains used, all expression vectors encoding each of the three heterologous proteins were multimerized in E. coli IC2015 strains in the course of cultivation, whereas the expression vectors containing the homologous gene never formed the plasmid multimers. The extent of multimerization was also dependent on a foreign gene insert in the expression vector. As a dominant effect of the SOS induction, recombinant plasmid vectors used for heterologous protein expression appear to significantly form various multimers in the recA+ E. coli host. © 2002 Wiley Periodicals, Inc. Biotechnol Bioeng 80: 84–92, 2002." @default.
• W2034246190 created "2016-06-24" @default.
• W2034246190 creator A5005659473 @default.
• W2034246190 creator A5009522689 @default.
• W2034246190 creator A5013322606 @default.
• W2034246190 creator A5014072218 @default.
• W2034246190 creator A5042455348 @default.
• W2034246190 creator A5065725770 @default.
• W2034246190 date "2002-08-15" @default.
• W2034246190 modified "2023-09-24" @default.
• W2034246190 title "Interplay of SOS induction, recombinant gene expression, and multimerization of plasmid vectors inEscherichia coli" @default.
• W2034246190 cites W1493386189 @default.
• W2034246190 cites W1526864164 @default.
• W2034246190 cites W1604146142 @default.
• W2034246190 cites W1604437810 @default.
• W2034246190 cites W1924241755 @default.
• W2034246190 cites W1966911475 @default.
• W2034246190 cites W1982063022 @default.
• W2034246190 cites W1995080182 @default.
• W2034246190 cites W1996002507 @default.
• W2034246190 cites W1999750537 @default.
• W2034246190 cites W2002369614 @default.
• W2034246190 cites W2004383792 @default.
• W2034246190 cites W2005702505 @default.
• W2034246190 cites W2016281336 @default.
• W2034246190 cites W2068518785 @default.
• W2034246190 cites W2070217882 @default.
• W2034246190 cites W2074034320 @default.
• W2034246190 cites W2089138944 @default.
• W2034246190 cites W2126394556 @default.
• W2034246190 cites W2140125340 @default.
• W2034246190 cites W2223128171 @default.
• W2034246190 cites W1999767883 @default.
• W2034246190 doi "https://doi.org/10.1002/bit.10354" @default.
• W2034246190 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12209789" @default.
• W2034246190 hasPublicationYear "2002" @default.
• W2034246190 type Work @default.
• W2034246190 sameAs 2034246190 @default.
• W2034246190 citedByCount "11" @default.
• W2034246190 countsByYear W20342461902017 @default.
• W2034246190 countsByYear W20342461902020 @default.
• W2034246190 countsByYear W20342461902023 @default.
• W2034246190 crossrefType "journal-article" @default.
• W2034246190 hasAuthorship W2034246190A5005659473 @default.
• W2034246190 hasAuthorship W2034246190A5009522689 @default.
• W2034246190 hasAuthorship W2034246190A5013322606 @default.
• W2034246190 hasAuthorship W2034246190A5014072218 @default.
• W2034246190 hasAuthorship W2034246190A5042455348 @default.
• W2034246190 hasAuthorship W2034246190A5065725770 @default.
• W2034246190 hasConcept C102744134 @default.
• W2034246190 hasConcept C104317684 @default.
• W2034246190 hasConcept C107618985 @default.
• W2034246190 hasConcept C108305142 @default.
• W2034246190 hasConcept C127413603 @default.
• W2034246190 hasConcept C145640855 @default.
• W2034246190 hasConcept C153911025 @default.
• W2034246190 hasConcept C168374021 @default.
• W2034246190 hasConcept C205225487 @default.
• W2034246190 hasConcept C22744801 @default.
• W2034246190 hasConcept C2780098074 @default.
• W2034246190 hasConcept C2780115692 @default.
• W2034246190 hasConcept C2780852648 @default.
• W2034246190 hasConcept C33161422 @default.
• W2034246190 hasConcept C40767141 @default.
• W2034246190 hasConcept C54355233 @default.
• W2034246190 hasConcept C547475151 @default.
• W2034246190 hasConcept C78519656 @default.
• W2034246190 hasConcept C86803240 @default.
• W2034246190 hasConcept C92087593 @default.
• W2034246190 hasConceptScore W2034246190C102744134 @default.
• W2034246190 hasConceptScore W2034246190C104317684 @default.
• W2034246190 hasConceptScore W2034246190C107618985 @default.
• W2034246190 hasConceptScore W2034246190C108305142 @default.
• W2034246190 hasConceptScore W2034246190C127413603 @default.
• W2034246190 hasConceptScore W2034246190C145640855 @default.
• W2034246190 hasConceptScore W2034246190C153911025 @default.
• W2034246190 hasConceptScore W2034246190C168374021 @default.
• W2034246190 hasConceptScore W2034246190C205225487 @default.
• W2034246190 hasConceptScore W2034246190C22744801 @default.
• W2034246190 hasConceptScore W2034246190C2780098074 @default.
• W2034246190 hasConceptScore W2034246190C2780115692 @default.
• W2034246190 hasConceptScore W2034246190C2780852648 @default.
• W2034246190 hasConceptScore W2034246190C33161422 @default.
• W2034246190 hasConceptScore W2034246190C40767141 @default.
• W2034246190 hasConceptScore W2034246190C54355233 @default.
• W2034246190 hasConceptScore W2034246190C547475151 @default.
• W2034246190 hasConceptScore W2034246190C78519656 @default.
• W2034246190 hasConceptScore W2034246190C86803240 @default.
• W2034246190 hasConceptScore W2034246190C92087593 @default.
• W2034246190 hasIssue "1" @default.
• W2034246190 hasLocation W20342461901 @default.
• W2034246190 hasLocation W20342461902 @default.
• W2034246190 hasOpenAccess W2034246190 @default.
• W2034246190 hasPrimaryLocation W20342461901 @default.
• W2034246190 hasRelatedWork W1983802182 @default.
• W2034246190 hasRelatedWork W1989770060 @default.
• W2034246190 hasRelatedWork W1997682515 @default.
• W2034246190 hasRelatedWork W2012875388 @default.

• next