FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034259107> ?p ?o ?g. }

• W2034259107 abstract "Abstract Background Adenovirus serotype 5 (Ad5) has many favourable characteristics for development as a gene therapy vector. However, the utility of current Ad5 vectors is limited by transient transgene expression, toxicity and immunogenicity. The most promising form of vector is the high capacity type, which is deleted for all viral genes. However, these vectors can only be produced to relatively low titres and with the aid of helper virus. Therefore a continuing challenge is the generation of more effective Ad5 vectors that can still be grown to high titres. Our approach is to generate complementing cell lines to support the growth of Ad5 vectors with novel late gene deficiencies. Results We have used LoxP/Cre recombination mediated cassette exchange (RMCE) to generate cell lines expressing Ad5 proteins encoded by the L4 region of the genome, the products of which play a pivotal role in the expression of Ad5 structural proteins. A panel of LoxP parent 293 cell lines was generated, each containing a GFP expression cassette under the control of a tetracycline-regulated promoter inserted at a random genome location; the cassette also contained a LoxP site between the promoter and GFP sequence. Clones displayed a variety of patterns of regulation, stability and level of GFP expression. Clone A1 was identified as a suitable parent for creation of inducible cell lines because of the tight inducibility and stability of its GFP expression. Using LoxP-targeted, Cre recombinase-mediated insertion of an L4 cassette to displace GFP from the regulated promoter in this parent clone, cell line A1-L4 was generated. This cell line expressed L4 100K, 22K and 33K proteins at levels sufficient to complement L4-33K mutant and L4-deleted viruses. Conclusions RMCE provides a method for rapid generation of Ad5 complementing cell lines from a pre-selected parental cell line, chosen for its desirable transgene expression characteristics. Parent cell lines can be selected for high or low gene expression, and for tight regulation, allowing viral protein expression to mirror that found during infection. Cell lines derived from a single parent will allow the growth of different vectors to be assessed without the complication of varying complementing protein expression." @default.
• W2034259107 created "2016-06-24" @default.
• W2034259107 creator A5050972490 @default.
• W2034259107 creator A5085856688 @default.
• W2034259107 creator A5089670625 @default.
• W2034259107 date "2010-12-01" @default.
• W2034259107 modified "2023-10-06" @default.
• W2034259107 title "Generation of Cell Lines to Complement Adenovirus Vectors using Recombination-Mediated Cassette Exchange" @default.
• W2034259107 cites W1586130277 @default.
• W2034259107 cites W1641628688 @default.
• W2034259107 cites W1921267352 @default.
• W2034259107 cites W1968384393 @default.
• W2034259107 cites W1971460945 @default.
• W2034259107 cites W1984441513 @default.
• W2034259107 cites W1985028933 @default.
• W2034259107 cites W1985849575 @default.
• W2034259107 cites W1992200158 @default.
• W2034259107 cites W1998950232 @default.
• W2034259107 cites W2002070063 @default.
• W2034259107 cites W2008260185 @default.
• W2034259107 cites W2013313513 @default.
• W2034259107 cites W2013413367 @default.
• W2034259107 cites W2016200465 @default.
• W2034259107 cites W2025616153 @default.
• W2034259107 cites W2032928525 @default.
• W2034259107 cites W2035690287 @default.
• W2034259107 cites W2044296969 @default.
• W2034259107 cites W2044629552 @default.
• W2034259107 cites W2060873330 @default.
• W2034259107 cites W2061657917 @default.
• W2034259107 cites W2068644182 @default.
• W2034259107 cites W2071970498 @default.
• W2034259107 cites W2081825650 @default.
• W2034259107 cites W2090429649 @default.
• W2034259107 cites W2096802570 @default.
• W2034259107 cites W2104975387 @default.
• W2034259107 cites W2109890939 @default.
• W2034259107 cites W2114458218 @default.
• W2034259107 cites W2121672863 @default.
• W2034259107 cites W2132282136 @default.
• W2034259107 cites W2133368279 @default.
• W2034259107 cites W2134738317 @default.
• W2034259107 cites W2144714494 @default.
• W2034259107 cites W2146611590 @default.
• W2034259107 cites W2151902657 @default.
• W2034259107 cites W2156581649 @default.
• W2034259107 cites W2159292745 @default.
• W2034259107 cites W2159694950 @default.
• W2034259107 cites W2164440528 @default.
• W2034259107 cites W2165896601 @default.
• W2034259107 cites W2166167705 @default.
• W2034259107 cites W2169402836 @default.
• W2034259107 cites W2169864486 @default.
• W2034259107 cites W3905809 @default.
• W2034259107 doi "https://doi.org/10.1186/1472-6750-10-92" @default.
• W2034259107 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3018391" @default.
• W2034259107 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21182761" @default.
• W2034259107 hasPublicationYear "2010" @default.
• W2034259107 type Work @default.
• W2034259107 sameAs 2034259107 @default.
• W2034259107 citedByCount "4" @default.
• W2034259107 countsByYear W20342591072019 @default.
• W2034259107 countsByYear W20342591072021 @default.
• W2034259107 crossrefType "journal-article" @default.
• W2034259107 hasAuthorship W2034259107A5050972490 @default.
• W2034259107 hasAuthorship W2034259107A5085856688 @default.
• W2034259107 hasAuthorship W2034259107A5089670625 @default.
• W2034259107 hasBestOaLocation W20342591071 @default.
• W2034259107 hasConcept C100012643 @default.
• W2034259107 hasConcept C10147152 @default.
• W2034259107 hasConcept C102230213 @default.
• W2034259107 hasConcept C104317684 @default.
• W2034259107 hasConcept C125929170 @default.
• W2034259107 hasConcept C141035611 @default.
• W2034259107 hasConcept C142613039 @default.
• W2034259107 hasConcept C153911025 @default.
• W2034259107 hasConcept C159047783 @default.
• W2034259107 hasConcept C22744801 @default.
• W2034259107 hasConcept C2776650774 @default.
• W2034259107 hasConcept C32470452 @default.
• W2034259107 hasConcept C40767141 @default.
• W2034259107 hasConcept C54355233 @default.
• W2034259107 hasConcept C86803240 @default.
• W2034259107 hasConcept C92087593 @default.
• W2034259107 hasConceptScore W2034259107C100012643 @default.
• W2034259107 hasConceptScore W2034259107C10147152 @default.
• W2034259107 hasConceptScore W2034259107C102230213 @default.
• W2034259107 hasConceptScore W2034259107C104317684 @default.
• W2034259107 hasConceptScore W2034259107C125929170 @default.
• W2034259107 hasConceptScore W2034259107C141035611 @default.
• W2034259107 hasConceptScore W2034259107C142613039 @default.
• W2034259107 hasConceptScore W2034259107C153911025 @default.
• W2034259107 hasConceptScore W2034259107C159047783 @default.
• W2034259107 hasConceptScore W2034259107C22744801 @default.
• W2034259107 hasConceptScore W2034259107C2776650774 @default.
• W2034259107 hasConceptScore W2034259107C32470452 @default.
• W2034259107 hasConceptScore W2034259107C40767141 @default.
• W2034259107 hasConceptScore W2034259107C54355233 @default.
• W2034259107 hasConceptScore W2034259107C86803240 @default.
• W2034259107 hasConceptScore W2034259107C92087593 @default.

• next