Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034262218> ?p ?o ?g. }
- W2034262218 endingPage "670" @default.
- W2034262218 startingPage "660" @default.
- W2034262218 abstract "Difference gel electrophoresis (DIGE) of fluorescently labelled human sperm proteins was used to identify diabetes- and obesity-associated changes of the sperm proteome. Semen samples from type 1 diabetics, non-diabetic obese individuals and a reference group of clinically healthy fertile donors were evaluated in a comparative study. The adaptation of a general protein extraction procedure to the solubilization of proteins from isolated progressively motile human spermatozoa resulted in the detection of approximately 2700 fluorescent protein spots in the DIGE images. Comparison of the patients' sperm proteomes with those of the reference group allowed the identification of 20 spots containing proteins that were present in the sperm lysates at significantly increased or decreased concentrations. In detail, eight of these spots were apparently related to type 1 diabetes while 12 spots were apparently related to obesity. Tryptic digestion of the spot proteins and mass spectrometric analysis of the corresponding peptides identified seven sperm proteins apparently associated with type 1 diabetes and nine sperm proteins apparently associated with obesity, three of which existing in multiple molecular forms. The established proteomic approach is expected to function as a non-invasive experimental tool in the diagnosis of male infertility and in monitoring any fertility-restoring therapy." @default.
- W2034262218 created "2016-06-24" @default.
- W2034262218 creator A5002485930 @default.
- W2034262218 creator A5018366034 @default.
- W2034262218 creator A5019967701 @default.
- W2034262218 creator A5048579827 @default.
- W2034262218 creator A5051790713 @default.
- W2034262218 creator A5075343984 @default.
- W2034262218 creator A5082379829 @default.
- W2034262218 creator A5088081229 @default.
- W2034262218 creator A5091610207 @default.
- W2034262218 date "2009-11-01" @default.
- W2034262218 modified "2023-10-14" @default.
- W2034262218 title "Identification of diabetes- and obesity-associated proteomic changes in human spermatozoa by difference gel electrophoresis" @default.
- W2034262218 cites W1536141833 @default.
- W2034262218 cites W1585312695 @default.
- W2034262218 cites W1964888298 @default.
- W2034262218 cites W1975840953 @default.
- W2034262218 cites W1978676217 @default.
- W2034262218 cites W1986400002 @default.
- W2034262218 cites W1995552079 @default.
- W2034262218 cites W2004884464 @default.
- W2034262218 cites W2015536666 @default.
- W2034262218 cites W2023816706 @default.
- W2034262218 cites W2025413292 @default.
- W2034262218 cites W2030404541 @default.
- W2034262218 cites W2042927678 @default.
- W2034262218 cites W2065992894 @default.
- W2034262218 cites W2066556286 @default.
- W2034262218 cites W2067895652 @default.
- W2034262218 cites W2079460324 @default.
- W2034262218 cites W2089706484 @default.
- W2034262218 cites W2090997573 @default.
- W2034262218 cites W2094513676 @default.
- W2034262218 cites W2106575294 @default.
- W2034262218 cites W2107033356 @default.
- W2034262218 cites W2108833696 @default.
- W2034262218 cites W2110065044 @default.
- W2034262218 cites W2118149443 @default.
- W2034262218 cites W2120898043 @default.
- W2034262218 cites W2121181475 @default.
- W2034262218 cites W2126267143 @default.
- W2034262218 cites W2139842655 @default.
- W2034262218 cites W2147769323 @default.
- W2034262218 cites W2151905911 @default.
- W2034262218 cites W2156479388 @default.
- W2034262218 cites W2159880763 @default.
- W2034262218 cites W2166998713 @default.
- W2034262218 cites W2170949754 @default.
- W2034262218 cites W2422146306 @default.
- W2034262218 cites W2471605942 @default.
- W2034262218 doi "https://doi.org/10.1016/j.rbmo.2009.07.001" @default.
- W2034262218 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20021714" @default.
- W2034262218 hasPublicationYear "2009" @default.
- W2034262218 type Work @default.
- W2034262218 sameAs 2034262218 @default.
- W2034262218 citedByCount "57" @default.
- W2034262218 countsByYear W20342622182012 @default.
- W2034262218 countsByYear W20342622182013 @default.
- W2034262218 countsByYear W20342622182014 @default.
- W2034262218 countsByYear W20342622182015 @default.
- W2034262218 countsByYear W20342622182016 @default.
- W2034262218 countsByYear W20342622182017 @default.
- W2034262218 countsByYear W20342622182018 @default.
- W2034262218 countsByYear W20342622182020 @default.
- W2034262218 countsByYear W20342622182021 @default.
- W2034262218 countsByYear W20342622182022 @default.
- W2034262218 countsByYear W20342622182023 @default.
- W2034262218 crossrefType "journal-article" @default.
- W2034262218 hasAuthorship W2034262218A5002485930 @default.
- W2034262218 hasAuthorship W2034262218A5018366034 @default.
- W2034262218 hasAuthorship W2034262218A5019967701 @default.
- W2034262218 hasAuthorship W2034262218A5048579827 @default.
- W2034262218 hasAuthorship W2034262218A5051790713 @default.
- W2034262218 hasAuthorship W2034262218A5075343984 @default.
- W2034262218 hasAuthorship W2034262218A5082379829 @default.
- W2034262218 hasAuthorship W2034262218A5088081229 @default.
- W2034262218 hasAuthorship W2034262218A5091610207 @default.
- W2034262218 hasBestOaLocation W20342622181 @default.
- W2034262218 hasConcept C104317684 @default.
- W2034262218 hasConcept C104397665 @default.
- W2034262218 hasConcept C126322002 @default.
- W2034262218 hasConcept C134018914 @default.
- W2034262218 hasConcept C16685009 @default.
- W2034262218 hasConcept C2777180221 @default.
- W2034262218 hasConcept C2777338322 @default.
- W2034262218 hasConcept C2777688143 @default.
- W2034262218 hasConcept C2778093475 @default.
- W2034262218 hasConcept C2779234561 @default.
- W2034262218 hasConcept C2781087480 @default.
- W2034262218 hasConcept C46111723 @default.
- W2034262218 hasConcept C54355233 @default.
- W2034262218 hasConcept C55493867 @default.
- W2034262218 hasConcept C555293320 @default.
- W2034262218 hasConcept C60644358 @default.
- W2034262218 hasConcept C71924100 @default.
- W2034262218 hasConcept C86803240 @default.
- W2034262218 hasConceptScore W2034262218C104317684 @default.