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• W2034263418 abstract "Helper-dependent adenovirus (HDAd), deleted in all viral protein-coding sequences has been designed to reduce immune response and favor long-term expression of therapeutic genes in clinical programs. Its production requires co-infection of E1-complementing cells with helper adenovirus (HAd). Significant progresses have been made in the molecular design of HDAd, but large scale production remains a challenge. In this work, a scalable system for HDAd production is designed and evaluated focusing on the co-infection step. A human embryo kidney 293 (293) derived cell line, the 293SF/FLPe was generated to produce efficiently HDAd while restricting the packaging of HAd. This cell line was adapted to grow in suspension and in serum-free medium. Multiplicity of infection (MOI) of HDAd ranging from 0.1 to 50 was evaluated in presence of HAd at a MOI of 5. Optimal MOIs for HDAd amplification were found in the range of 5–10. HAd contamination was only 1%. These results were validated in a 3 L bioreactor under controlled operating conditions where a higher HDAd yield of 2.6 × 109 viral particles (VP)/mL or 3.5 × 108 infectious units (IU)/mL of HDAd was obtained." @default.
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• W2034263418 date "2008-03-01" @default.
• W2034263418 modified "2023-09-26" @default.
• W2034263418 title "Development of a suspension serum-free helper-dependent adenovirus production system and assessment of co-infection conditions" @default.
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• W2034263418 doi "https://doi.org/10.1016/j.jviromet.2007.10.017" @default.
• W2034263418 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18079009" @default.
• W2034263418 hasPublicationYear "2008" @default.
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