FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034266275> ?p ?o ?g. }

• W2034266275 endingPage "62" @default.
• W2034266275 startingPage "56" @default.
• W2034266275 abstract "OBJECTIVE: The objective of this study was to evaluate the hypothesis that ketoacids (acetoacetic acid and β-hydroxybutyrate) diminish glucose transport in trophoblasts cultured from first-trimester chorionic villi. STUDY DESIGN: First-trimester trophoblasts were obtained by transabdominal chorionic villus sampling for subsequent cytogenetic analysis. The cells were established as a continuous line exhibiting trophoblast characteristics. Trophoblasts were cultured in Ham's F12/Dulbecco's modified Eagle's medium (1:1) supplemented with 15% fetal bovine serum. Experiments were initiated by a 24-hour preincubation in serum-free Ham's F12/Dulbecco's modified Eagle's medium followed by incubation with ketoacids (acetoacetic acid and β-hydroxybutyrate, 0 to 10 mmol/L) in the presence or absence of insulin-like growth factor-I (100 ng/ml). The cells were challenged with 2-deoxy-[1,2-3H]D-glucose (0.1 mmol/L) for 5 minutes and then cell-associated radioactivity was measured. Total ribonucleic acid was extracted from cells incubated with ketoacids in the presence or absence of insulin-like growth factor-I, and Northern blots were probed with a phosphorus 32 - labeled complementary deoxyribonucleic acid fragment encoding the rat GLUT 1. RESULTS: Ketoacids caused a dose-dependent inhibition of glucose transport. At 5 mmol/L acetoacetic acid there was a >50% reduction in the rate of glucose transport in both control and insulin-like growth factor-I - treated cells. The diminution in glucose uptake by trophoblasts was not due to cellular toxicity of the ketoacids because there was no significant difference in trypan blue exclusion or lactate dehydrogenase release between control and ketoacid-treated cells. Northern analysis revealed that the steady-state expression of GLUT1 messenger ribonucleic acid was diminished in ketone-treated cells, but this effect was overcome by coincubation of cultures with insulin or insulin-like growth factor-I. CONCLUSIONS: These results indicate that ketoacids can suppress the uptake of glucose into first-trimester human trophoblasts. Because ketoacidosis in pregnant women with diabetes mellitus is a frequent clinical consequence of poor metabolic control, it is possible that elevated levels of acetoacetic acid and β-hydroxybutyrate may impair the transport of glucose across the placental trophoblast and into the fetal circulation. (Am J Obstet Gynecol 1996;175:56-62.)" @default.
• W2034266275 created "2016-06-24" @default.
• W2034266275 creator A5000368756 @default.
• W2034266275 creator A5003908929 @default.
• W2034266275 creator A5004375804 @default.
• W2034266275 creator A5028628051 @default.
• W2034266275 creator A5072118682 @default.
• W2034266275 date "1996-07-01" @default.
• W2034266275 modified "2023-09-24" @default.
• W2034266275 title "Ketoacids attenuate glucose uptake in human trophoblasts isolated from first-trimester chorionic villi" @default.
• W2034266275 cites W1257903918 @default.
• W2034266275 cites W1519345624 @default.
• W2034266275 cites W1553600579 @default.
• W2034266275 cites W1975219187 @default.
• W2034266275 cites W1981445209 @default.
• W2034266275 cites W1994527360 @default.
• W2034266275 cites W2022338193 @default.
• W2034266275 cites W2024985631 @default.
• W2034266275 cites W2081421902 @default.
• W2034266275 cites W2134467817 @default.
• W2034266275 cites W2178164598 @default.
• W2034266275 cites W2304764269 @default.
• W2034266275 cites W2331721697 @default.
• W2034266275 cites W4235678011 @default.
• W2034266275 cites W4293247451 @default.
• W2034266275 cites W4294216491 @default.
• W2034266275 doi "https://doi.org/10.1016/s0002-9378(96)70251-x" @default.
• W2034266275 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8694076" @default.
• W2034266275 hasPublicationYear "1996" @default.
• W2034266275 type Work @default.
• W2034266275 sameAs 2034266275 @default.
• W2034266275 citedByCount "6" @default.
• W2034266275 countsByYear W20342662752016 @default.
• W2034266275 countsByYear W20342662752021 @default.
• W2034266275 countsByYear W20342662752022 @default.
• W2034266275 crossrefType "journal-article" @default.
• W2034266275 hasAuthorship W2034266275A5000368756 @default.
• W2034266275 hasAuthorship W2034266275A5003908929 @default.
• W2034266275 hasAuthorship W2034266275A5004375804 @default.
• W2034266275 hasAuthorship W2034266275A5028628051 @default.
• W2034266275 hasAuthorship W2034266275A5072118682 @default.
• W2034266275 hasConcept C126322002 @default.
• W2034266275 hasConcept C134018914 @default.
• W2034266275 hasConcept C1491633281 @default.
• W2034266275 hasConcept C16685009 @default.
• W2034266275 hasConcept C170493617 @default.
• W2034266275 hasConcept C172680121 @default.
• W2034266275 hasConcept C185592680 @default.
• W2034266275 hasConcept C25642318 @default.
• W2034266275 hasConcept C2775960820 @default.
• W2034266275 hasConcept C2776953305 @default.
• W2034266275 hasConcept C2777495146 @default.
• W2034266275 hasConcept C2779234561 @default.
• W2034266275 hasConcept C2779404806 @default.
• W2034266275 hasConcept C2780070996 @default.
• W2034266275 hasConcept C2781385326 @default.
• W2034266275 hasConcept C54355233 @default.
• W2034266275 hasConcept C55493867 @default.
• W2034266275 hasConcept C71924100 @default.
• W2034266275 hasConcept C81885089 @default.
• W2034266275 hasConcept C86803240 @default.
• W2034266275 hasConceptScore W2034266275C126322002 @default.
• W2034266275 hasConceptScore W2034266275C134018914 @default.
• W2034266275 hasConceptScore W2034266275C1491633281 @default.
• W2034266275 hasConceptScore W2034266275C16685009 @default.
• W2034266275 hasConceptScore W2034266275C170493617 @default.
• W2034266275 hasConceptScore W2034266275C172680121 @default.
• W2034266275 hasConceptScore W2034266275C185592680 @default.
• W2034266275 hasConceptScore W2034266275C25642318 @default.
• W2034266275 hasConceptScore W2034266275C2775960820 @default.
• W2034266275 hasConceptScore W2034266275C2776953305 @default.
• W2034266275 hasConceptScore W2034266275C2777495146 @default.
• W2034266275 hasConceptScore W2034266275C2779234561 @default.
• W2034266275 hasConceptScore W2034266275C2779404806 @default.
• W2034266275 hasConceptScore W2034266275C2780070996 @default.
• W2034266275 hasConceptScore W2034266275C2781385326 @default.
• W2034266275 hasConceptScore W2034266275C54355233 @default.
• W2034266275 hasConceptScore W2034266275C55493867 @default.
• W2034266275 hasConceptScore W2034266275C71924100 @default.
• W2034266275 hasConceptScore W2034266275C81885089 @default.
• W2034266275 hasConceptScore W2034266275C86803240 @default.
• W2034266275 hasIssue "1" @default.
• W2034266275 hasLocation W20342662751 @default.
• W2034266275 hasLocation W20342662752 @default.
• W2034266275 hasOpenAccess W2034266275 @default.
• W2034266275 hasPrimaryLocation W20342662751 @default.
• W2034266275 hasRelatedWork W1969918132 @default.
• W2034266275 hasRelatedWork W2002562299 @default.
• W2034266275 hasRelatedWork W2061541548 @default.
• W2034266275 hasRelatedWork W2063078815 @default.
• W2034266275 hasRelatedWork W2098923832 @default.
• W2034266275 hasRelatedWork W2099734673 @default.
• W2034266275 hasRelatedWork W2104394567 @default.
• W2034266275 hasRelatedWork W2391196319 @default.
• W2034266275 hasRelatedWork W2588905940 @default.
• W2034266275 hasRelatedWork W4285305568 @default.
• W2034266275 hasVolume "175" @default.
• W2034266275 isParatext "false" @default.

• next