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- W2034268768 abstract "Oat β-glucan was partially degraded with (1→4)-β-d-glucan 4-glucanohydrolase for different periods of time. Weight average molecular weight and weight average intrinsic viscosity were obtained from SEC-RI-RALLS-Visc and ranged from 2200 to 213,900 g/mol and 7 to 316 ml/g, respectively. The viscosity equation determined [η̄]w=1.06×10−2M̄w0.86 indicated an extended random coil conformation. When the coil was modelled as a worm-like chain a persistent length of 3.65 nm was obtained. The hydrolysis products identified after extensive degradation were glucose, cellobiose, laminaribiose, 4-O-β-laminaribiosyl d-glucose, 4-O-β-laminaribiosyl d-cellobiose and 3-O-β-cellobiosyl d-cellobiose showing that other enzyme activities were present. The depolymerization kinetics suggested that longer sequences of consecutive (1→4)-linkages represent fast hydrolysable sites and the presence of higher relative proportions of (1→3)-linkages restricts the affinity of the enzyme." @default.
- W2034268768 created "2016-06-24" @default.
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- W2034268768 date "2001-11-01" @default.
- W2034268768 modified "2023-10-15" @default.
- W2034268768 title "Molecular weight, structure and shape of oat (1→3),(1→4)-β-d-glucan fractions obtained by enzymatic degradation with (1→4)-β-d-glucan 4-glucanohydrolase from Trichoderma reesei" @default.
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- W2034268768 doi "https://doi.org/10.1016/s0144-8617(00)00329-5" @default.
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