FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034271898> ?p ?o ?g. }

• W2034271898 endingPage "464" @default.
• W2034271898 startingPage "455" @default.
• W2034271898 abstract "The proteolysis of the human epidermal growth factor receptor cytoplasmic domain by calpain has been studied in vitro using purified recombinant cytoplasmic domain expressed in insect cells. Limited proteolysis produced kinase that was truncated at either N- or C-termini, as well as in the hinge region. We identified seven sites of calpain proteolysis by N-terminal sequencing of purified fragments. Calpain cleaved between the catalytic and autophosphorylation domains at two sites in the sequence Gln996–Asp1059, in the hinge region. Three new sites were also found in the autophosphorylation domain, preceding each of the major autophosphorylation sites. A fourth new site was located in the juxtamembrane domain, C-terminal to the regulatory Thr654. We purified an active 42-kDa fragment generated by calpain proteolysis between Leu659–Gln660 in the juxtamembrane domain, and in the hinge region. A fifth new site of calpain cleavage was found between the nucleotide binding motif Gly-Xaa-Gly-Xaa-Xaa-Gly and the essential Lys721 in the catalytic core of the kinase. Since both of these features are required for catalysis, calpain cleavage at this site may potentially provide a mechanism for down-regulation of kinase activity in vivo, under conditions of calpain activation. Thus the distribution of calpain cleavage sites along the kinase domain is consistent with a role for calpain both as a processing and as a degradative protease in epidermal growth factor receptor signalling." @default.
• W2034271898 created "2016-06-24" @default.
• W2034271898 creator A5005782454 @default.
• W2034271898 creator A5022967413 @default.
• W2034271898 creator A5066870453 @default.
• W2034271898 creator A5090986303 @default.
• W2034271898 date "1994-07-01" @default.
• W2034271898 modified "2023-09-23" @default.
• W2034271898 title "The calpain cleavage sites in the epidermal growth factor receptor kinase domain" @default.
• W2034271898 cites W116697568 @default.
• W2034271898 cites W1225587815 @default.
• W2034271898 cites W1483470782 @default.
• W2034271898 cites W1489057868 @default.
• W2034271898 cites W1543218984 @default.
• W2034271898 cites W1548966243 @default.
• W2034271898 cites W1549264844 @default.
• W2034271898 cites W1552470397 @default.
• W2034271898 cites W1556750259 @default.
• W2034271898 cites W1557403719 @default.
• W2034271898 cites W1560887909 @default.
• W2034271898 cites W1565471250 @default.
• W2034271898 cites W1567148937 @default.
• W2034271898 cites W1578456230 @default.
• W2034271898 cites W1592646667 @default.
• W2034271898 cites W1595879864 @default.
• W2034271898 cites W1605892702 @default.
• W2034271898 cites W1932052686 @default.
• W2034271898 cites W1966502030 @default.
• W2034271898 cites W1968013329 @default.
• W2034271898 cites W1972960760 @default.
• W2034271898 cites W1975085160 @default.
• W2034271898 cites W1981468237 @default.
• W2034271898 cites W1984351623 @default.
• W2034271898 cites W1990001508 @default.
• W2034271898 cites W1995337883 @default.
• W2034271898 cites W1995574514 @default.
• W2034271898 cites W2014332691 @default.
• W2034271898 cites W2018754034 @default.
• W2034271898 cites W2026326676 @default.
• W2034271898 cites W2032422012 @default.
• W2034271898 cites W2035584408 @default.
• W2034271898 cites W2044075747 @default.
• W2034271898 cites W2049834913 @default.
• W2034271898 cites W2050710256 @default.
• W2034271898 cites W2056681493 @default.
• W2034271898 cites W2061671663 @default.
• W2034271898 cites W2069314886 @default.
• W2034271898 cites W2078494160 @default.
• W2034271898 cites W2080964580 @default.
• W2034271898 cites W2084136914 @default.
• W2034271898 cites W2106747452 @default.
• W2034271898 cites W2117185170 @default.
• W2034271898 cites W2120179279 @default.
• W2034271898 cites W2145533030 @default.
• W2034271898 cites W2150971857 @default.
• W2034271898 cites W2164682544 @default.
• W2034271898 cites W2167198814 @default.
• W2034271898 cites W240647565 @default.
• W2034271898 cites W2441335220 @default.
• W2034271898 cites W26952851 @default.
• W2034271898 cites W307860981 @default.
• W2034271898 cites W4238018483 @default.
• W2034271898 doi "https://doi.org/10.1111/j.1432-1033.1994.tb19013.x" @default.
• W2034271898 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8055914" @default.
• W2034271898 hasPublicationYear "1994" @default.
• W2034271898 type Work @default.
• W2034271898 sameAs 2034271898 @default.
• W2034271898 citedByCount "36" @default.
• W2034271898 countsByYear W20342718982012 @default.
• W2034271898 countsByYear W20342718982013 @default.
• W2034271898 countsByYear W20342718982014 @default.
• W2034271898 countsByYear W20342718982018 @default.
• W2034271898 countsByYear W20342718982021 @default.
• W2034271898 crossrefType "journal-article" @default.
• W2034271898 hasAuthorship W2034271898A5005782454 @default.
• W2034271898 hasAuthorship W2034271898A5022967413 @default.
• W2034271898 hasAuthorship W2034271898A5066870453 @default.
• W2034271898 hasAuthorship W2034271898A5090986303 @default.
• W2034271898 hasConcept C104317684 @default.
• W2034271898 hasConcept C143065580 @default.
• W2034271898 hasConcept C150109051 @default.
• W2034271898 hasConcept C151730666 @default.
• W2034271898 hasConcept C153911025 @default.
• W2034271898 hasConcept C170493617 @default.
• W2034271898 hasConcept C175156509 @default.
• W2034271898 hasConcept C181199279 @default.
• W2034271898 hasConcept C184235292 @default.
• W2034271898 hasConcept C185592680 @default.
• W2034271898 hasConcept C2776362946 @default.
• W2034271898 hasConcept C2781307694 @default.
• W2034271898 hasConcept C32619005 @default.
• W2034271898 hasConcept C43369102 @default.
• W2034271898 hasConcept C55493867 @default.
• W2034271898 hasConcept C56928146 @default.
• W2034271898 hasConcept C86803240 @default.
• W2034271898 hasConcept C95444343 @default.
• W2034271898 hasConcept C97029542 @default.
• W2034271898 hasConceptScore W2034271898C104317684 @default.

• next