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- W2034275328 abstract "A strategy for expression and purification of recombinant N-terminal human trefoil factor family-domain peptide 3 (hTFF3) in Escherichia coli was established. The gene of hTFF3 was synthesized to substitute the low-usage condons with corresponding high-usage synonymous condons. At the same time, the signal peptide of DsbC was added to the N-terminus of the hTFF3 gene. The mature recombinant hTFF3 was located in the periplasm of E. coli, which can be released by sonication. The protein was further purified by a two-step cation exchange chromatography mentod. The yield is about 14–15 mg/l of culture. The biological activity of purified hTFF3 was analyzed by cell-based apoptosis assay, which shows that the recombinant hTFF3 is biologically active." @default.
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- W2034275328 date "2005-07-01" @default.
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- W2034275328 title "High-level expression of human TFF3 in Escherichia coli" @default.
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- W2034275328 doi "https://doi.org/10.1016/j.peptides.2005.04.012" @default.
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