Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034276369> ?p ?o ?g. }
Showing items 1 to 70 of
70
with 100 items per page.
- W2034276369 abstract "Proceedings: AACR Annual Meeting 2014; April 5-9, 2014; San Diego, CAType I interferons (IFNs) are secreted by infected cells in response to the recognition of nucleic acids. DNA damage and the ensuing DNA damage response also induce the expression of IFNs by poorly understood mechanisms. Here we show the presence of extramitochondrial single-stranded (ss) and double-stranded (ds) DNA in the cytosol of mouse and human tumor cells. The DNA only partially co-localized with autophagosomes, stress granules or lysosomes, but a majority was associated with mitochondria. Genotoxic agents increased the amount of DNA in the cytosol of tumor cells with low levels of constitutive cytosolic DNA. Cloning of ssDNA and dsDNA present in the cytosol of mouse tumor cells showed that at least some of the cytosolic DNA is derived from unique genomic loci. Cloned cytosolic DNA sequences often contained genomic retroelements. In silico analysis of the cloned DNA indicated that cytosolic DNA is predicted to form putative triplex DNA structures. Overexpression of Rnaseh1, which degrades intermolecular triplex structures called R-loops, reduced the levels of cytosolic ssDNA and dsDNA, while overexpression of Trex1, the major 3’-5’ DNA exonuclease, decreased the amount of cytosolic ssDNA but not dsDNA. Upregulation of IFNs in response to genotoxic agents was impaired in cells expressing Rnaseh1 or Trex1. In summary, we provide evidence that DNA damage in tumor cells induces the accumulation of genomic DNA in the cytosol and the production of type I IFNs in many tumor cells, which may contribute to immunosurveillance of cancers.Citation Format: Yu J. Shen, Nina Le Bert, Christine XE Koo, Ho SW Samantha, Ken J. Ishii, David H. Raulet, Stephan Gasser. Tumor cells express genome-derived DNA in the cytosol. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 3168. doi:10.1158/1538-7445.AM2014-3168" @default.
- W2034276369 created "2016-06-24" @default.
- W2034276369 creator A5013918402 @default.
- W2034276369 creator A5028873086 @default.
- W2034276369 creator A5031327184 @default.
- W2034276369 creator A5034837493 @default.
- W2034276369 creator A5054960247 @default.
- W2034276369 creator A5058734512 @default.
- W2034276369 creator A5063767722 @default.
- W2034276369 date "2014-09-30" @default.
- W2034276369 modified "2023-09-28" @default.
- W2034276369 title "Abstract 3168: Tumor cells express genome-derived DNA in the cytosol" @default.
- W2034276369 doi "https://doi.org/10.1158/1538-7445.am2014-3168" @default.
- W2034276369 hasPublicationYear "2014" @default.
- W2034276369 type Work @default.
- W2034276369 sameAs 2034276369 @default.
- W2034276369 citedByCount "0" @default.
- W2034276369 crossrefType "proceedings-article" @default.
- W2034276369 hasAuthorship W2034276369A5013918402 @default.
- W2034276369 hasAuthorship W2034276369A5028873086 @default.
- W2034276369 hasAuthorship W2034276369A5031327184 @default.
- W2034276369 hasAuthorship W2034276369A5034837493 @default.
- W2034276369 hasAuthorship W2034276369A5054960247 @default.
- W2034276369 hasAuthorship W2034276369A5058734512 @default.
- W2034276369 hasAuthorship W2034276369A5063767722 @default.
- W2034276369 hasConcept C143425029 @default.
- W2034276369 hasConcept C153911025 @default.
- W2034276369 hasConcept C17757408 @default.
- W2034276369 hasConcept C181199279 @default.
- W2034276369 hasConcept C552990157 @default.
- W2034276369 hasConcept C55493867 @default.
- W2034276369 hasConcept C86803240 @default.
- W2034276369 hasConcept C95444343 @default.
- W2034276369 hasConcept C98539663 @default.
- W2034276369 hasConceptScore W2034276369C143425029 @default.
- W2034276369 hasConceptScore W2034276369C153911025 @default.
- W2034276369 hasConceptScore W2034276369C17757408 @default.
- W2034276369 hasConceptScore W2034276369C181199279 @default.
- W2034276369 hasConceptScore W2034276369C552990157 @default.
- W2034276369 hasConceptScore W2034276369C55493867 @default.
- W2034276369 hasConceptScore W2034276369C86803240 @default.
- W2034276369 hasConceptScore W2034276369C95444343 @default.
- W2034276369 hasConceptScore W2034276369C98539663 @default.
- W2034276369 hasLocation W20342763691 @default.
- W2034276369 hasOpenAccess W2034276369 @default.
- W2034276369 hasPrimaryLocation W20342763691 @default.
- W2034276369 hasRelatedWork W1959105989 @default.
- W2034276369 hasRelatedWork W2009115701 @default.
- W2034276369 hasRelatedWork W2020911697 @default.
- W2034276369 hasRelatedWork W2022417639 @default.
- W2034276369 hasRelatedWork W2026677489 @default.
- W2034276369 hasRelatedWork W2042663808 @default.
- W2034276369 hasRelatedWork W2043165259 @default.
- W2034276369 hasRelatedWork W2047348882 @default.
- W2034276369 hasRelatedWork W2060785257 @default.
- W2034276369 hasRelatedWork W2061087634 @default.
- W2034276369 hasRelatedWork W2082416672 @default.
- W2034276369 hasRelatedWork W2102512189 @default.
- W2034276369 hasRelatedWork W2105126975 @default.
- W2034276369 hasRelatedWork W2114745290 @default.
- W2034276369 hasRelatedWork W2117204059 @default.
- W2034276369 hasRelatedWork W2147054512 @default.
- W2034276369 hasRelatedWork W2149663149 @default.
- W2034276369 hasRelatedWork W2172207516 @default.
- W2034276369 hasRelatedWork W2474026003 @default.
- W2034276369 hasRelatedWork W2578144844 @default.
- W2034276369 isParatext "false" @default.
- W2034276369 isRetracted "false" @default.
- W2034276369 magId "2034276369" @default.
- W2034276369 workType "article" @default.