FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034276997> ?p ?o ?g. }

• W2034276997 endingPage "177" @default.
• W2034276997 startingPage "168" @default.
• W2034276997 abstract "This paper describes a rapid and simple technique for the simultaneous quantitative analysis of PGE2, PGE3, and other closely related prostaglandins from cultured cells using liquid chromatography/electrospray ionization tandem mass spectrometry. This method permits quantification of selected individual prostaglandins derived either from arachidonic acid (AA) or eicosapentaenoic acid (EPA) from cell extracts without tedious derivatization, lengthy sample preparation, and separation required by GC-MS- or HPLC-UV-based methods. The validation assessment showed that the quantitative determination is linear (r2>0.999) for both PGE2 and PGE3 in the range tested (1–500 ng/ml, 0.0028–1.4 μM) and a coefficient of variation lower than 10% was obtained for samples analyzed on 3 separate days. The detection limit was 2.5 pg for both PGE2 and PGE3. Extraction efficiency of PGE2 and PGE3 from cell suspensions ranged from 89.4 to 98.2%. As an application of the method, prostaglandins formed by EPA in human lung cancer A549 cells were determined. A 62% reduction of PGE2 formation was noted when A549 cells were treated with 100 μM of EPA. Concomitantly, EPA increased formation of PGE3 by 10-fold in A549 cells. This is the first report that unequivocally demonstrates that EPA can be converted to PGE3 by cyclooxygenase in human cancer cells." @default.
• W2034276997 created "2016-06-24" @default.
• W2034276997 creator A5003077528 @default.
• W2034276997 creator A5003664704 @default.
• W2034276997 creator A5003693436 @default.
• W2034276997 creator A5083929738 @default.
• W2034276997 creator A5086839893 @default.
• W2034276997 date "2002-09-01" @default.
• W2034276997 modified "2023-09-22" @default.
• W2034276997 title "Quantitative high-performance liquid chromatography/electrospray ionization tandem mass spectrometric analysis of 2- and 3-series prostaglandins in cultured tumor cells" @default.
• W2034276997 cites W111839144 @default.
• W2034276997 cites W139849764 @default.
• W2034276997 cites W1968310022 @default.
• W2034276997 cites W1973320574 @default.
• W2034276997 cites W1974221742 @default.
• W2034276997 cites W1979009074 @default.
• W2034276997 cites W1984757473 @default.
• W2034276997 cites W1991398536 @default.
• W2034276997 cites W1994132178 @default.
• W2034276997 cites W2000545849 @default.
• W2034276997 cites W2007227223 @default.
• W2034276997 cites W2008300906 @default.
• W2034276997 cites W2015007509 @default.
• W2034276997 cites W2021630670 @default.
• W2034276997 cites W2031343440 @default.
• W2034276997 cites W2040308284 @default.
• W2034276997 cites W2047850339 @default.
• W2034276997 cites W2053171203 @default.
• W2034276997 cites W2062813587 @default.
• W2034276997 cites W2073042812 @default.
• W2034276997 cites W2074521053 @default.
• W2034276997 cites W2080492086 @default.
• W2034276997 cites W2085316933 @default.
• W2034276997 cites W2087744847 @default.
• W2034276997 cites W2094737934 @default.
• W2034276997 cites W2100649229 @default.
• W2034276997 cites W2134692293 @default.
• W2034276997 cites W2146708145 @default.
• W2034276997 cites W2148190114 @default.
• W2034276997 cites W2149233326 @default.
• W2034276997 cites W2153801889 @default.
• W2034276997 doi "https://doi.org/10.1016/s0003-2697(02)00218-x" @default.
• W2034276997 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12234478" @default.
• W2034276997 hasPublicationYear "2002" @default.
• W2034276997 type Work @default.
• W2034276997 sameAs 2034276997 @default.
• W2034276997 citedByCount "70" @default.
• W2034276997 countsByYear W20342769972012 @default.
• W2034276997 countsByYear W20342769972013 @default.
• W2034276997 countsByYear W20342769972014 @default.
• W2034276997 countsByYear W20342769972015 @default.
• W2034276997 countsByYear W20342769972016 @default.
• W2034276997 countsByYear W20342769972017 @default.
• W2034276997 countsByYear W20342769972018 @default.
• W2034276997 countsByYear W20342769972019 @default.
• W2034276997 countsByYear W20342769972020 @default.
• W2034276997 crossrefType "journal-article" @default.
• W2034276997 hasAuthorship W2034276997A5003077528 @default.
• W2034276997 hasAuthorship W2034276997A5003664704 @default.
• W2034276997 hasAuthorship W2034276997A5003693436 @default.
• W2034276997 hasAuthorship W2034276997A5083929738 @default.
• W2034276997 hasAuthorship W2034276997A5086839893 @default.
• W2034276997 hasConcept C1491633281 @default.
• W2034276997 hasConcept C162356407 @default.
• W2034276997 hasConcept C179998833 @default.
• W2034276997 hasConcept C181199279 @default.
• W2034276997 hasConcept C185592680 @default.
• W2034276997 hasConcept C194451485 @default.
• W2034276997 hasConcept C2776923573 @default.
• W2034276997 hasConcept C2778078955 @default.
• W2034276997 hasConcept C31827203 @default.
• W2034276997 hasConcept C40684141 @default.
• W2034276997 hasConcept C43617362 @default.
• W2034276997 hasConcept C55493867 @default.
• W2034276997 hasConcept C81729549 @default.
• W2034276997 hasConcept C95986675 @default.
• W2034276997 hasConceptScore W2034276997C1491633281 @default.
• W2034276997 hasConceptScore W2034276997C162356407 @default.
• W2034276997 hasConceptScore W2034276997C179998833 @default.
• W2034276997 hasConceptScore W2034276997C181199279 @default.
• W2034276997 hasConceptScore W2034276997C185592680 @default.
• W2034276997 hasConceptScore W2034276997C194451485 @default.
• W2034276997 hasConceptScore W2034276997C2776923573 @default.
• W2034276997 hasConceptScore W2034276997C2778078955 @default.
• W2034276997 hasConceptScore W2034276997C31827203 @default.
• W2034276997 hasConceptScore W2034276997C40684141 @default.
• W2034276997 hasConceptScore W2034276997C43617362 @default.
• W2034276997 hasConceptScore W2034276997C55493867 @default.
• W2034276997 hasConceptScore W2034276997C81729549 @default.
• W2034276997 hasConceptScore W2034276997C95986675 @default.
• W2034276997 hasIssue "1" @default.
• W2034276997 hasLocation W20342769971 @default.
• W2034276997 hasLocation W20342769972 @default.
• W2034276997 hasOpenAccess W2034276997 @default.
• W2034276997 hasPrimaryLocation W20342769971 @default.
• W2034276997 hasRelatedWork W1965060487 @default.
• W2034276997 hasRelatedWork W1980225556 @default.
• W2034276997 hasRelatedWork W2004572792 @default.

• next