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• W2034286345 endingPage "206" @default.
• W2034286345 startingPage "181" @default.
• W2034286345 abstract "The interaction between cyclophosphamide hydrochloride (CYC) and aspirin (ASA) with human serum albumin (HSA) was studied by various kind of spectroscopic, ζ potential and molecular modeling under physiological conditions. The fluorescence data showed that the binding of drugs to proteins caused strong static fluorescence quenching. The analysis of the fluorescence quenching of HSA in the binary and ternary systems displayed that ASA was affected by the complex formed between CYC and HSA. Moreover, CYC was influenced by the HSA-ASA complex. The inherent binding information, including the quenching mechanism, binding constants, number of binding sites, effective quenching constant, fraction of the initial fluorescence and thermodynamic parameters were measured by the fluorescence quenching technique at various temperatures. In addition, according to the synchronous fluorescence spectra of HSA, the results showed that the fluorescence quenching of HSA originated from the Trp and Tyr residues, and indicated a conformational change of HSA with the addition of the drugs. Far-UV CD spectra of HSA were recorded before and after the addition of ASA and CYC as binary and ternary systems. An increase in intensity of the positive CD peak of HSA was observed in the presence of the drugs. The results were interpreted by excited interactions between the aromatic residues of the HSA binding sites and the drugs bound to them. The distance r between donor and acceptor was obtained by the Forster energy according to fluorescence resonance energy transfer (FRET) and found to be 2.35 nm and 1.78 nm for CYC and ASA, respectively. This confirmed the existence of static quenching for proteins in the presence of CYC and ASA. Furthermore, docking studies pointed at a reduction of the affinity of each of the drug compounds to the protein in the presence of the other in meaningful amounts. Pre-binding of any of the said compounds forced the second to bind in a non-optimized location and orientation. The potential at the electrokinetic shear surface of the protein-drug solution were measured at several concentrations of the drugs by the ζ potential technique, which confirmed experimental and theoretical results." @default.
• W2034286345 created "2016-06-24" @default.
• W2034286345 creator A5003917239 @default.
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• W2034286345 date "2011-08-01" @default.
• W2034286345 modified "2023-10-16" @default.
• W2034286345 title "Investigations with Spectroscopy, Zeta Potential and Molecular Modeling of the Non-Cooperative Behaviour Between Cyclophosphamide Hydrochloride and Aspirin upon Interaction with Human Serum Albumin: Binary and Ternary Systems from the View Point of Multi-Drug Therapy" @default.
• W2034286345 cites W1964900539 @default.
• W2034286345 cites W1965346197 @default.
• W2034286345 cites W1966145381 @default.
• W2034286345 cites W1968271729 @default.
• W2034286345 cites W1970262488 @default.
• W2034286345 cites W1972193118 @default.
• W2034286345 cites W1977848104 @default.
• W2034286345 cites W1980134992 @default.
• W2034286345 cites W1982631880 @default.
• W2034286345 cites W1984766916 @default.
• W2034286345 cites W1986850697 @default.
• W2034286345 cites W1988691988 @default.
• W2034286345 cites W1989046847 @default.
• W2034286345 cites W1991450922 @default.
• W2034286345 cites W1992045220 @default.
• W2034286345 cites W1994064285 @default.
• W2034286345 cites W1994136277 @default.
• W2034286345 cites W1994521301 @default.
• W2034286345 cites W1994695140 @default.
• W2034286345 cites W1995705219 @default.
• W2034286345 cites W1996536752 @default.
• W2034286345 cites W2003161137 @default.
• W2034286345 cites W2004458222 @default.
• W2034286345 cites W2004485898 @default.
• W2034286345 cites W2008166117 @default.
• W2034286345 cites W2009442799 @default.
• W2034286345 cites W2010311727 @default.
• W2034286345 cites W2012985887 @default.
• W2034286345 cites W2013673509 @default.
• W2034286345 cites W2014529535 @default.
• W2034286345 cites W2015861038 @default.
• W2034286345 cites W2018503874 @default.
• W2034286345 cites W2019764682 @default.
• W2034286345 cites W2024050173 @default.
• W2034286345 cites W2024601290 @default.
• W2034286345 cites W2024684631 @default.
• W2034286345 cites W2031426081 @default.
• W2034286345 cites W2031487554 @default.
• W2034286345 cites W2032139821 @default.
• W2034286345 cites W2036294206 @default.
• W2034286345 cites W2043049669 @default.
• W2034286345 cites W2043267427 @default.
• W2034286345 cites W2046214657 @default.
• W2034286345 cites W2046228060 @default.
• W2034286345 cites W2046513459 @default.
• W2034286345 cites W2048347161 @default.
• W2034286345 cites W2048716650 @default.
• W2034286345 cites W2049830971 @default.
• W2034286345 cites W2050673079 @default.
• W2034286345 cites W2051098113 @default.
• W2034286345 cites W2055033066 @default.
• W2034286345 cites W2055379081 @default.
• W2034286345 cites W2055597111 @default.
• W2034286345 cites W2056420715 @default.
• W2034286345 cites W2060089658 @default.
• W2034286345 cites W2060197871 @default.
• W2034286345 cites W2060567277 @default.
• W2034286345 cites W2061859853 @default.
• W2034286345 cites W2063256200 @default.
• W2034286345 cites W2063563180 @default.
• W2034286345 cites W2065305851 @default.
• W2034286345 cites W2068226693 @default.
• W2034286345 cites W2071072200 @default.
• W2034286345 cites W2071692975 @default.
• W2034286345 cites W2074806467 @default.
• W2034286345 cites W2075380413 @default.
• W2034286345 cites W2075705506 @default.
• W2034286345 cites W2075717225 @default.
• W2034286345 cites W2076268932 @default.
• W2034286345 cites W2077486563 @default.
• W2034286345 cites W2077973224 @default.
• W2034286345 cites W2078606218 @default.
• W2034286345 cites W2079592758 @default.
• W2034286345 cites W2080812344 @default.
• W2034286345 cites W2084410838 @default.
• W2034286345 cites W2085023615 @default.
• W2034286345 cites W2085395323 @default.
• W2034286345 cites W2085472460 @default.
• W2034286345 cites W2085984495 @default.
• W2034286345 cites W2088625277 @default.
• W2034286345 cites W2090486698 @default.
• W2034286345 cites W2092366654 @default.
• W2034286345 cites W2097535648 @default.
• W2034286345 cites W2124241917 @default.
• W2034286345 cites W2138334249 @default.

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