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• W2034291133 abstract "Growth of the acidophilic archaeon, “Ferroplasma acidarmanus” strain fer1, in a laboratory medium (primary constituents, FeSO4 72 mM and 0.02% yeast extract) is minimal. A survey of the annotated genome revealed metabolic transporters for Ni2+, sugars, and amino acids. Accordingly, the concentration of yeast extract was increased to 0.1% and the addition of 2 mM Ni(NH4)2(SO4)2 significantly enhanced the cultivation of strain fer1. The maximum optical density in the modified fer1 medium (mfer) was OD492=0.27 with 1010 viable cells / ml as determined by a most-probable-number method, which exceeds previously reported viable cells / ml by >100-fold. Strain fer1 displayed chemolithotrophic growth with Fe2+ in mfer containing 100 mM FeSO4 or FeCl2. In the absence of Fe2+, heterotrophic growth occurred with one of the following salts (100 mM): ZnSO4, MnSO4, MgSO4, (NH4)2SO4, or Fe2(SO4)3, and did not occur with (100 mM): ZnCl2, MnCl2, MgCl2, NH4Cl, or FeCl3. Escaping headspace gas from strain fer1 cultures formed a precipitate in a zinc acetate trap. Sulfide was absent in the precipitate but zinc and sulfur were detected. These data demonstrate that SO4 is required for heterotrophic growth of strain fer1 and may have a role in the global sulfur cycle." @default.
• W2034291133 created "2016-06-24" @default.
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• W2034291133 date "2005-05-01" @default.
• W2034291133 modified "2023-10-18" @default.
• W2034291133 title "Sulfate requirement for heterotrophic growth of “Ferroplasma acidarmanus” strain fer1" @default.
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• W2034291133 doi "https://doi.org/10.1016/j.resmic.2004.12.007" @default.
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