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• W2034293882 endingPage "D1184" @default.
• W2034293882 startingPage "D1176" @default.
• W2034293882 abstract "Reversible phosphorylation is a key mechanism for regulating protein function. Thus it is of high interest to know which kinase can phosphorylate which proteins. Comprehensive information about phosphorylation sites in Arabidopsis proteins is hosted within the PhosPhAt database (http://phosphat.mpimp-golm.mpg.de). However, our knowledge of the kinases that phosphorylate those sites is dispersed throughout the literature and very difficult to access, particularly for investigators seeking to interpret large scale and high-throughput experiments. Therefore, we aimed to compile information on kinase–substrate interactions and kinase-specific regulatory information and make this available via a new functionality embedded in PhosPhAt. Our approach involved systematic surveying of the literature for regulatory information on the members of the major kinase families in Arabidopsis thaliana, such as CDPKs, MPK(KK)s, AGC kinases and SnRKs, as well as individual kinases from other families. To date, we have researched more than 4450 kinase-related publications, which collectively contain information on about 289 kinases. Users can now query the PhosPhAt database not only for experimental and predicted phosphorylation sites of individual proteins, but also for known substrates for a given kinase or kinase family. Further developments include addition of new phosphorylation sites and visualization of clustered phosphorylation events, known as phosphorylation hotspots." @default.
• W2034293882 created "2016-06-24" @default.
• W2034293882 creator A5050044367 @default.
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• W2034293882 date "2012-11-19" @default.
• W2034293882 modified "2023-09-24" @default.
• W2034293882 title "PhosPhAt goes kinases—searchable protein kinase target information in the plant phosphorylation site database PhosPhAt" @default.
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• W2034293882 doi "https://doi.org/10.1093/nar/gks1081" @default.
• W2034293882 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3531128" @default.
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• W2034293882 hasPublicationYear "2012" @default.
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