Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034295773> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2034295773 endingPage "131" @default.
- W2034295773 startingPage "126" @default.
- W2034295773 abstract "The reaction of almond β-glucosidase with p-nitrophenyl-β-D-glucoside has been investigated over the temperature range +25° to −45° using 50% aqueous dimethyl sulfoxide (DMSO) as solvent. At temperatures below those at which turnover occurs a “burst” of p-nitrophenol proportional to the enzyme concentration is observed. Such a “burst” suggests the existence of a glucosyl-enzyme intermediate whose breakdown is rate-limiting, and provides a method for measuring the active-site normality. At pH 5.9, 25°, the presence of 50% DMSO causes an increase in Km from 1.7×10−3M (0%) to 1.7×10−2M, whereas Vmax is unchanged. The DMSO thus apparently acts as a competitive inhibitor with Ki = 0.7M. The Arrhenius plot for turnover is linear over the accessible temperature range with Ea = 23.0 ± 2.0 kcal/mole." @default.
- W2034295773 created "2016-06-24" @default.
- W2034295773 creator A5011397738 @default.
- W2034295773 creator A5061603180 @default.
- W2034295773 date "1974-05-01" @default.
- W2034295773 modified "2023-09-27" @default.
- W2034295773 title "Evidence for a glucosyl-enzyme intermediate in the β-glucosidase-catalyzed hydrolysis of p-Nitrophenyl-β-D-Glucoside" @default.
- W2034295773 cites W2007602043 @default.
- W2034295773 cites W2032748445 @default.
- W2034295773 cites W203763077 @default.
- W2034295773 cites W2075196356 @default.
- W2034295773 cites W2092331199 @default.
- W2034295773 cites W2093959164 @default.
- W2034295773 cites W2402446434 @default.
- W2034295773 cites W2410188648 @default.
- W2034295773 doi "https://doi.org/10.1016/0006-291x(74)90900-0" @default.
- W2034295773 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/4831060" @default.
- W2034295773 hasPublicationYear "1974" @default.
- W2034295773 type Work @default.
- W2034295773 sameAs 2034295773 @default.
- W2034295773 citedByCount "23" @default.
- W2034295773 crossrefType "journal-article" @default.
- W2034295773 hasAuthorship W2034295773A5011397738 @default.
- W2034295773 hasAuthorship W2034295773A5061603180 @default.
- W2034295773 hasConcept C124815738 @default.
- W2034295773 hasConcept C127413603 @default.
- W2034295773 hasConcept C142724271 @default.
- W2034295773 hasConcept C155647269 @default.
- W2034295773 hasConcept C161790260 @default.
- W2034295773 hasConcept C178790620 @default.
- W2034295773 hasConcept C181199279 @default.
- W2034295773 hasConcept C184651966 @default.
- W2034295773 hasConcept C185592680 @default.
- W2034295773 hasConcept C188198153 @default.
- W2034295773 hasConcept C204787440 @default.
- W2034295773 hasConcept C2778452553 @default.
- W2034295773 hasConcept C2780471494 @default.
- W2034295773 hasConcept C2781347565 @default.
- W2034295773 hasConcept C43617362 @default.
- W2034295773 hasConcept C71240020 @default.
- W2034295773 hasConcept C71924100 @default.
- W2034295773 hasConcept C78519656 @default.
- W2034295773 hasConcept C86183883 @default.
- W2034295773 hasConcept C8739425 @default.
- W2034295773 hasConcept C94412978 @default.
- W2034295773 hasConcept C95121573 @default.
- W2034295773 hasConceptScore W2034295773C124815738 @default.
- W2034295773 hasConceptScore W2034295773C127413603 @default.
- W2034295773 hasConceptScore W2034295773C142724271 @default.
- W2034295773 hasConceptScore W2034295773C155647269 @default.
- W2034295773 hasConceptScore W2034295773C161790260 @default.
- W2034295773 hasConceptScore W2034295773C178790620 @default.
- W2034295773 hasConceptScore W2034295773C181199279 @default.
- W2034295773 hasConceptScore W2034295773C184651966 @default.
- W2034295773 hasConceptScore W2034295773C185592680 @default.
- W2034295773 hasConceptScore W2034295773C188198153 @default.
- W2034295773 hasConceptScore W2034295773C204787440 @default.
- W2034295773 hasConceptScore W2034295773C2778452553 @default.
- W2034295773 hasConceptScore W2034295773C2780471494 @default.
- W2034295773 hasConceptScore W2034295773C2781347565 @default.
- W2034295773 hasConceptScore W2034295773C43617362 @default.
- W2034295773 hasConceptScore W2034295773C71240020 @default.
- W2034295773 hasConceptScore W2034295773C71924100 @default.
- W2034295773 hasConceptScore W2034295773C78519656 @default.
- W2034295773 hasConceptScore W2034295773C86183883 @default.
- W2034295773 hasConceptScore W2034295773C8739425 @default.
- W2034295773 hasConceptScore W2034295773C94412978 @default.
- W2034295773 hasConceptScore W2034295773C95121573 @default.
- W2034295773 hasIssue "1" @default.
- W2034295773 hasLocation W20342957731 @default.
- W2034295773 hasLocation W20342957732 @default.
- W2034295773 hasOpenAccess W2034295773 @default.
- W2034295773 hasPrimaryLocation W20342957731 @default.
- W2034295773 hasRelatedWork W1875901600 @default.
- W2034295773 hasRelatedWork W2000102722 @default.
- W2034295773 hasRelatedWork W2034295773 @default.
- W2034295773 hasRelatedWork W2043534497 @default.
- W2034295773 hasRelatedWork W2065460068 @default.
- W2034295773 hasRelatedWork W2075375450 @default.
- W2034295773 hasRelatedWork W2077986889 @default.
- W2034295773 hasRelatedWork W2086068589 @default.
- W2034295773 hasRelatedWork W2951570289 @default.
- W2034295773 hasRelatedWork W2460503865 @default.
- W2034295773 hasVolume "58" @default.
- W2034295773 isParatext "false" @default.
- W2034295773 isRetracted "false" @default.
- W2034295773 magId "2034295773" @default.
- W2034295773 workType "article" @default.