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- W2034296037 abstract "Design and construction of biochemical pathways has increased the complexity of biosynthetically-produced compounds when compared to single enzyme biocatalysis. However, the coordination of multiple enzymes can introduce a complicated set of obstacles to overcome in order to achieve a high titer and yield of the desired compound. Metabolic engineering has made great strides in developing tools to optimize the flux through a target pathway, but the inherent characteristics of a particular enzyme within the pathway can still limit the productivity. Thus, judicious protein design is critical for metabolic and pathway engineering. This review will describe various strategies and examples of applying protein design to pathway engineering to optimize the flux through the pathway. The proteins can be engineered for altered substrate specificity/selectivity, increased catalytic activity, reduced mass transfer limitations through specific protein localization, and reduced substrate/product inhibition. Protein engineering can also be expanded to design biosensors to enable high through-put screening and to customize cell signaling networks. These strategies have successfully engineered pathways for significantly increased productivity of the desired product or in the production of novel compounds." @default.
- W2034296037 created "2016-06-24" @default.
- W2034296037 creator A5014465828 @default.
- W2034296037 creator A5024169462 @default.
- W2034296037 creator A5052493543 @default.
- W2034296037 date "2014-02-01" @default.
- W2034296037 modified "2023-09-25" @default.
- W2034296037 title "Protein design for pathway engineering" @default.
- W2034296037 cites W1491585327 @default.
- W2034296037 cites W1547106545 @default.
- W2034296037 cites W1897627367 @default.
- W2034296037 cites W1964521061 @default.
- W2034296037 cites W1966403223 @default.
- W2034296037 cites W1967901576 @default.
- W2034296037 cites W1968602937 @default.
- W2034296037 cites W1969350272 @default.
- W2034296037 cites W1973494721 @default.
- W2034296037 cites W1974993545 @default.
- W2034296037 cites W1978119727 @default.
- W2034296037 cites W1978806418 @default.
- W2034296037 cites W1979626519 @default.
- W2034296037 cites W1979705207 @default.
- W2034296037 cites W1981471795 @default.
- W2034296037 cites W1984180361 @default.
- W2034296037 cites W1986714487 @default.
- W2034296037 cites W1987696643 @default.
- W2034296037 cites W1989948255 @default.
- W2034296037 cites W1995118863 @default.
- W2034296037 cites W1996629685 @default.
- W2034296037 cites W2001602167 @default.
- W2034296037 cites W2003058717 @default.
- W2034296037 cites W2005329081 @default.
- W2034296037 cites W2005616487 @default.
- W2034296037 cites W2005862188 @default.
- W2034296037 cites W2007966650 @default.
- W2034296037 cites W2008649912 @default.
- W2034296037 cites W2013971421 @default.
- W2034296037 cites W2016099222 @default.
- W2034296037 cites W2024720831 @default.
- W2034296037 cites W2029943478 @default.
- W2034296037 cites W2032349705 @default.
- W2034296037 cites W2033196044 @default.
- W2034296037 cites W2034717680 @default.
- W2034296037 cites W2034846223 @default.
- W2034296037 cites W2036894128 @default.
- W2034296037 cites W2037261732 @default.
- W2034296037 cites W2038269938 @default.
- W2034296037 cites W2043854809 @default.
- W2034296037 cites W2044610162 @default.
- W2034296037 cites W2047360542 @default.
- W2034296037 cites W2049541231 @default.
- W2034296037 cites W2053403225 @default.
- W2034296037 cites W2055194955 @default.
- W2034296037 cites W2060782384 @default.
- W2034296037 cites W2061647355 @default.
- W2034296037 cites W2065796273 @default.
- W2034296037 cites W2066381187 @default.
- W2034296037 cites W2069571028 @default.
- W2034296037 cites W2073265884 @default.
- W2034296037 cites W2077309097 @default.
- W2034296037 cites W2078272501 @default.
- W2034296037 cites W2079260562 @default.
- W2034296037 cites W2079347688 @default.
- W2034296037 cites W2079574042 @default.
- W2034296037 cites W2087004701 @default.
- W2034296037 cites W2089011164 @default.
- W2034296037 cites W2092059846 @default.
- W2034296037 cites W2098671352 @default.
- W2034296037 cites W2106769579 @default.
- W2034296037 cites W2106951530 @default.
- W2034296037 cites W2117179092 @default.
- W2034296037 cites W2118714546 @default.
- W2034296037 cites W2120706216 @default.
- W2034296037 cites W2124546559 @default.
- W2034296037 cites W2132238954 @default.
- W2034296037 cites W2134783465 @default.
- W2034296037 cites W2146061786 @default.
- W2034296037 cites W2146372898 @default.
- W2034296037 cites W2147310107 @default.
- W2034296037 cites W2147516046 @default.
- W2034296037 cites W2149202606 @default.
- W2034296037 cites W2150665661 @default.
- W2034296037 cites W2156654543 @default.
- W2034296037 cites W2156911822 @default.
- W2034296037 cites W2160522393 @default.
- W2034296037 cites W2164189687 @default.
- W2034296037 cites W2168059482 @default.
- W2034296037 cites W2169450274 @default.
- W2034296037 cites W2169989540 @default.
- W2034296037 doi "https://doi.org/10.1016/j.jsb.2013.03.011" @default.
- W2034296037 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3732524" @default.
- W2034296037 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23558037" @default.
- W2034296037 hasPublicationYear "2014" @default.
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