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• W2034296064 startingPage "4972" @default.
• W2034296064 abstract "Copy-choice RNA recombination occurs during viral RNA synthesis when the viral transcription complex switches templates. We demonstrate that RNA-dependent RNA polymerase from bovine viral diarrhea virus and the replicases from three plant-infecting RNA viruses can produce easily detectable recombination products in vitr o by switching templates during elongative RNA synthesis. Template sequence and/or structure, and NTP availability affected the frequency of template switch by the transcription complex. Our results provide biochemical support for copy-choice recombination and establish assays for mechanistic analyses of intermolecular RNA recombination in vitro ." @default.
• W2034296064 created "2016-06-24" @default.
• W2034296064 creator A5032589056 @default.
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• W2034296064 date "2001-04-17" @default.
• W2034296064 modified "2023-09-27" @default.
• W2034296064 title "Factors regulating template switch <i>in vitro</i> by viral RNA-dependent RNA polymerases: Implications for RNA–RNA recombination" @default.
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• W2034296064 doi "https://doi.org/10.1073/pnas.081077198" @default.
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