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• W2034302809 abstract "We compared the proliferation of neonatal and adult airway smooth muscle cells (ASMC) with no/moderate lung disease, in glucose- (energy production by glycolysis) or glucose-free medium (ATP production from mitochondrial oxidative phosphorylations only), in response to 10% fetal calf serum (FCS) and PDGF-AA. In the presence of glucose, cell counts were significantly greater in neonatal vs. adult ASMC. Similarly, neonatal ASMC DNA synthesis in 10% FCS and PDGF-AA, and [Ca2+]i responses in the presence of histamine were significantly enhanced vs. adults. In glucose-free medium, cell proliferation was preserved in neonatal cells, unlike in adult cells, with concomitant increased porin (an indicator of mitochondrial activity) protein expression. Compared to adults, stimulated neonatal human ASMC are in a rapid and robust proliferative phase and have the capacity to respond disproportionately under abnormal environmental conditions, through increased mitochondrial biogenesis and altered calcium homeostasis." @default.
• W2034302809 created "2016-06-24" @default.
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• W2034302809 date "2015-03-23" @default.
• W2034302809 modified "2023-10-18" @default.
• W2034302809 title "An Age-Wise Comparison of Human Airway Smooth Muscle Proliferative Capacity" @default.
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• W2034302809 doi "https://doi.org/10.1371/journal.pone.0122446" @default.
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