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• W2034302976 startingPage "995" @default.
• W2034302976 abstract "Phytases are the primary enzymes responsible for the hydrolysis of phytic acid, myo-inositol-1, 2, 3, 4, 5, 6-hexakisphosphate (InsP6). The pathway of hydrolysis of InsP6 by phytase from wheat bran of Triticum aestivum L. cv. Nourin #61 is proved in this study. Structures of the intermediates were established by a variety of nuclear magnetic resonance techniques (1H-, two-dimensional 1H-1H coupling-correlation spectra and two-dimensional 31P-1H correlation spectra), gas chromatography, and bioassay. On the basis of the structures identified, initial hydrolysis of the phosphate ester occurs at the D/L-4 position of InsP6 to yield D/L-Ins (1, 2, 3, 5, 6) P5. After the dephosphorylation, the pathway of dephosphorylation is divided into two routes. The main route proceeds via D/L-Ins (1, 2, 5, 6) P4, D/L-Ins (1, 2, 6) P3 and D/L-Ins (1, 2) P2, while the minor route proceeds via D/L-Ins (1, 2, 3, 6) P4, Ins (1, 2, 3) P3 and D/L-Ins (1, 2) P2. D/L-Ins (1, 2) P2 is hydrolyzed at the D/L-1 or 2-position, and finally myo-inositol is produced." @default.
• W2034302976 created "2016-06-24" @default.
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• W2034302976 date "2000-01-01" @default.
• W2034302976 modified "2023-09-27" @default.
• W2034302976 title "The Pathway of Dephosphorylation of<i>myo</i>-Inositol Hexakisphosphate by Phytases from Wheat Bran of<i>Triticum aestivum</i>L. cv. Nourin #61" @default.
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• W2034302976 doi "https://doi.org/10.1271/bbb.64.995" @default.
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