FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034305006> ?p ?o ?g. }

• W2034305006 endingPage "20" @default.
• W2034305006 startingPage "12" @default.
• W2034305006 abstract "The aim of the present study is to investigate the effect of genistein on human neuroblastoma SK-N-MC cells. MTT proliferation assay, LDH cytotoxicity assay, flow cytometric analysis, real-time quantitative RT-PCR and western blotting were used to investigate the effect of genistein on cell survival, cellular toxicity, cell cycle progression, and mRNA and protein alterations of selected DNA damage-, cell cycle- and apoptosis-related genes in SK-N-MC cells. Genistein suppressed cell proliferation, increased LDH release and modulated cell cycle distribution through accumulation of cells at G2/M- and S-phase and sub-G0 (cell death) with a concurrent decrease of cells at G0/G1 phase. Genistein increased the MDC1 (Mediator of DNA damage Checkpoint protein 1), p53, p21waf1/cip1, Cdc2 and Bax mRNA levels in a dose-dependent manner. However, PLK1 (Polo-Like Kinase 1) and Cyclin B1 mRNAs were down-regulated after genistein treatment. Furthermore, Genistein did not alter Chk2 (Checkpoint Kinase 2), Bcl-2 and Cdc25C mRNA levels. On western blotting analyses; genistein increased the protein level of MDC1, p53, p21waf1/cip1, and Bax in a dose-dependent manner. Genistein also increased the phosphorylation of Chk2 and Cdc25C at Thr-68 and Ser-216, respectively. In addition, consistently with PLK1 down-regulation, the phosphorylation of Cdc25C at Ser-198 was markedly decreased after genistein treatment. Additionally, Chk2, Cdc25C, Cyclin B1, p-Cyclin B1 (Ser-147), and Cdc2 as well as Bcl-2 proteins were down-regulated after genistein treatment. Altogether, these results suggest for the first time the involvement of MDC1 up-regulation after genistein treatment in DNA damage-induced Chk2 activation- and PLK1 down-regulation-mediated apoptosis and cell cycle checkpoint pathways." @default.
• W2034305006 created "2016-06-24" @default.
• W2034305006 creator A5013488718 @default.
• W2034305006 creator A5015812353 @default.
• W2034305006 creator A5050767709 @default.
• W2034305006 creator A5065565198 @default.
• W2034305006 creator A5087330131 @default.
• W2034305006 date "2007-12-01" @default.
• W2034305006 modified "2023-09-25" @default.
• W2034305006 title "Genistein-induced neuronal apoptosis and G2/M cell cycle arrest is associated with MDC1 up-regulation and PLK1 down-regulation" @default.
• W2034305006 cites W119784748 @default.
• W2034305006 cites W1537737236 @default.
• W2034305006 cites W1965239527 @default.
• W2034305006 cites W1971476329 @default.
• W2034305006 cites W1974190379 @default.
• W2034305006 cites W1976683836 @default.
• W2034305006 cites W1980017984 @default.
• W2034305006 cites W1980658808 @default.
• W2034305006 cites W1982305402 @default.
• W2034305006 cites W1985407849 @default.
• W2034305006 cites W1986141898 @default.
• W2034305006 cites W1997294285 @default.
• W2034305006 cites W1998461392 @default.
• W2034305006 cites W2002707923 @default.
• W2034305006 cites W2008720155 @default.
• W2034305006 cites W2014646550 @default.
• W2034305006 cites W2016552344 @default.
• W2034305006 cites W2020830322 @default.
• W2034305006 cites W2022301159 @default.
• W2034305006 cites W2028044172 @default.
• W2034305006 cites W2028140459 @default.
• W2034305006 cites W2039271361 @default.
• W2034305006 cites W2040235495 @default.
• W2034305006 cites W2041912628 @default.
• W2034305006 cites W2051163818 @default.
• W2034305006 cites W2052791307 @default.
• W2034305006 cites W2059212267 @default.
• W2034305006 cites W2062646762 @default.
• W2034305006 cites W2063702398 @default.
• W2034305006 cites W2068384749 @default.
• W2034305006 cites W2074056525 @default.
• W2034305006 cites W2077709632 @default.
• W2034305006 cites W2079585875 @default.
• W2034305006 cites W2083139178 @default.
• W2034305006 cites W2092307522 @default.
• W2034305006 cites W2104788350 @default.
• W2034305006 cites W2107277218 @default.
• W2034305006 cites W2116480637 @default.
• W2034305006 cites W2120730389 @default.
• W2034305006 cites W2131837045 @default.
• W2034305006 cites W2131962932 @default.
• W2034305006 cites W2151392747 @default.
• W2034305006 cites W2170959793 @default.
• W2034305006 cites W2319415470 @default.
• W2034305006 cites W4323053775 @default.
• W2034305006 cites W2018388108 @default.
• W2034305006 doi "https://doi.org/10.1016/j.ejphar.2007.07.039" @default.
• W2034305006 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17706963" @default.
• W2034305006 hasPublicationYear "2007" @default.
• W2034305006 type Work @default.
• W2034305006 sameAs 2034305006 @default.
• W2034305006 citedByCount "45" @default.
• W2034305006 countsByYear W20343050062012 @default.
• W2034305006 countsByYear W20343050062013 @default.
• W2034305006 countsByYear W20343050062014 @default.
• W2034305006 countsByYear W20343050062015 @default.
• W2034305006 countsByYear W20343050062017 @default.
• W2034305006 countsByYear W20343050062018 @default.
• W2034305006 countsByYear W20343050062019 @default.
• W2034305006 countsByYear W20343050062020 @default.
• W2034305006 countsByYear W20343050062022 @default.
• W2034305006 crossrefType "journal-article" @default.
• W2034305006 hasAuthorship W2034305006A5013488718 @default.
• W2034305006 hasAuthorship W2034305006A5015812353 @default.
• W2034305006 hasAuthorship W2034305006A5050767709 @default.
• W2034305006 hasAuthorship W2034305006A5065565198 @default.
• W2034305006 hasAuthorship W2034305006A5087330131 @default.
• W2034305006 hasConcept C105696609 @default.
• W2034305006 hasConcept C120504264 @default.
• W2034305006 hasConcept C134018914 @default.
• W2034305006 hasConcept C153911025 @default.
• W2034305006 hasConcept C184235292 @default.
• W2034305006 hasConcept C190283241 @default.
• W2034305006 hasConcept C2778298627 @default.
• W2034305006 hasConcept C2778437423 @default.
• W2034305006 hasConcept C2910325170 @default.
• W2034305006 hasConcept C29537977 @default.
• W2034305006 hasConcept C502942594 @default.
• W2034305006 hasConcept C54086632 @default.
• W2034305006 hasConcept C55493867 @default.
• W2034305006 hasConcept C62112901 @default.
• W2034305006 hasConcept C86803240 @default.
• W2034305006 hasConcept C95444343 @default.
• W2034305006 hasConceptScore W2034305006C105696609 @default.
• W2034305006 hasConceptScore W2034305006C120504264 @default.
• W2034305006 hasConceptScore W2034305006C134018914 @default.
• W2034305006 hasConceptScore W2034305006C153911025 @default.
• W2034305006 hasConceptScore W2034305006C184235292 @default.

• next